Studies on Regulation of Macrophages Function during Nidation-Molecular and Cell Biological Studies-)

着床过程中巨噬细胞功能调控的研究-分子与细胞生物学研究-)

基本信息

批准号：
07044182
负责人：
TACHI Chikashi
金额：
$ 9.22万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for international Scientific Research
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07044182/
关键词：
Placenta Implantation Macrophages Luminal epithelial cells Trophoblast cells Cell culture Signal transduction Cytokines Transgenic animals シバヤギ子宮上皮細胞トランスジェニック MMP MAPキナーゼ

项目摘要

The in vitro manipulation of mammalian embryos, including the introduction of exogenous genes, will be considered to be complete, in many instances, only after the successful embryo transfer leading to the birth of newborns. It has been well recognized, however, that the enbryo transfer suffers from poor success rates, the implantation processes being the notorious bottle neck. In order to prevent the implantation failure, the elucidation of the mechanisms underlying the cellular or molecular processes of implantation will be essential. The main purpose of the present Israel-Japan bi-national co-operation projects is to acquire the Israeli expertise and information concerning the macrophage research and the production of transgenic goats.We have attempted to establish in vitro cultured cell lines of trophoblast cells, the luminal epithelial cells of the endometrium and macrophages of Shiba goats in order to work out the in vitro co-culture systems which will serve as a model to analyze … More the influence of macrophages upon the implantation and subsequent placetation processes. We could establish the cultured cell lines of caprine luminal epithelial cells, although they have not yet been cloned. The caprine luminal epithelial cells undergo formation of gland-like structures in vitro, and some of the cells were seen to differentiate into secretory cells possessing full of secretory granules in the cytoplasm. Experiments are underway to clone these epithelial cell lines.Trophoblast cell cultures suffered from the sudden reduction in the proliferative capacities, after passages of several passages. attempts are being made to restore the proliferative capacities of the putative trophoblast cell lines.Macrophages of Shiba goats turned out to be difficult to obtain either from the bone marrow or from the spleen. Owing to some unknown reasons, the caprine macrophages appear to behave differently from the murine cells. Further experiments are needed to establish methodology for the collection and culture of the caprine macrophages.On Israeli side, biochemical analysis of the signal transduction systems which operate during the activation of macrophages in currently in progress.Exchange of valuable up-to-date information concerning the experiments in not only macrophages but also production of transgenic goats, possible application of MRI methods in implantation reserach and in vitro maturation of murine oocytes, were done. Less

在许多情况下，只有在成功的胚胎转移导致新生儿诞生之后，在许多情况下，哺乳动物胚胎的体外操纵（包括引入外源基因）才能被认为是完整的。然而，人们已经众所周知，埃布里（Enbryo）转移的成功率很差，植入过程是臭名昭著的瓶颈。 In order to prevent the implantation failure, the elucidation of the mechanisms underlying the cellular or molecular The main purpose of the present Israel-Japan bi-national co-operation projects is to acquire the Israeli expertise and information concerning the macrophage research and the production of transgenic goats.We have attempted to establish in vitro cultured cell lines of trophoblast cells, the luminal epithelial cells of the endometrium and北山羊的巨噬细胞是为了制定体外共培养系统，该系统将作为分析的模型……更多的是巨噬细胞对植入和随后的安置过程的影响。尽管尚未克隆，但我们可以建立Caprine Luminal上皮细胞的培养细胞系。孢子腔腔上皮细胞在体外经历腺样结构的形成，一些细胞被认为分化为具有细胞质中分泌颗粒的秘书细胞。实验正在进行这些上皮细胞系的克隆。经过多个通道的传递后，细胞增生能力的突然降低。正在尝试恢复推定的滋养细胞细胞系的增殖能力。史希巴山羊的巨噬细胞很难从骨髓或脾脏中获得。由于某些未知原因，Caprine巨噬细胞的行为与鼠细胞的行为不同。需要进一步的实验来建立方法论，以在以色列方面，对信号转导系统的生物化学分析，在当前正在进行中激活巨噬细胞期间运行的信号转导系统的生物化学分析。对有价值的有价值的最新信息，不仅是巨噬细胞中的实验，还可以构成MRIM的实验，并在MRI上构成MRI方法，并在MRI中构成MRI方法，以实现MRI方法，以实现MRI的方法。鼠卵母细胞完成。较少的