NUTRITION METABOLISM OF HIGHER ANIMALS IN HYPOXIA

高等动物缺氧时的营养代谢

• 批准号: 05660146
• 负责人: NAKANO Yoshihisa
• 金额: $ 1.47万
• 依托单位: Osaka Prefecture University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05660146/
• 关键词: Gastrin; Gastric acid secretion; Gastric emptying; Normobaric hypoxia; Rats; 低酸素; 胃酸; 胃排出遅延; 赤血球; スーパーオキシドジスムターゼ; カタラーゼ; 抗酸化的防御機構

This study examined the effects of hypoxia on the gastric function in conscious rats in a series of experiments. Hypoxia delayd the excretion rats of urinary urea until after 12h of hypoxia and the delay was more remarkable in rats subjected to 7.6%O_2 hypoxia than in those subjected to 10.5%O_2 hypoxia. In Experiment 2, when rats fed the high protein diet for 4h were exposed to 7.6%O_2, a delay in gastric emptying was found in the hypoxic rats. In Experiment 3, when rats fed the normal protein diet for 4h were exposed to 7.6%O_2 hypoxia, the rats of urea excretion in the hypoxic rats were not significantly different from that in the normoxic rats until after 24h of hypoxia. However, rats fed the normal protein diet for 4h werecaused a delay of gastric emptying by 10.5%O_2 hypoxia. In Experiment 4, when rats fed the high protein diet for 4h were exposed to 7.6%O_2 or 10.5%O_2 hypoxia, the plasma gastrin concentration in the 7.6%O_2 hypoxic rats was 2.3-fold that in the normoxic rats after 6h of hypoxia although the gastrin level did not differ between the 10.5%O_2 hypoxic and the normoxic rats during 6h of hopoxia. Furthermore, when food-deprived rats were exposed to 7.6%O_2 or 10.5%O_2 hypoxia, the gastrin concentration was higher in both hypoxic groups than in the normoxic group after 3h of hypoxia. In Experiment 5, food-deprived rats with pylous ligation were exposed to 7.6%O_2 or 10.5%O_2 hypoxia for 3h. Both hypoxia inhibited the secretion of gastric acid and stimulated the gastrin release. In Experiment 6, food-deprived rats with pylorus ligation were orally administered HC1 and then exposed to 7.6%O_2 hypoxia for 3h. The rise of the gastrin concentration due to hypoxia was completely inhibited by the oral administration of HC1. These results demonstrate that hypoxia inhibits gastric emptying and secretion and that the inhibitory effect of hypoxia on gastric acid secretion stimulates gastrin release through positive feedback regulation.

本研究通过一系列实验研究了缺氧对清醒大鼠胃功能的影响。缺氧使大鼠尿素排泄延迟至缺氧12h后，且7.6%O_2缺氧大鼠的延迟较10.5%O_2缺氧大鼠更为显着。实验2中,当大鼠喂食高蛋白饲料4h并暴露于7.6%O_2时,发现缺氧大鼠胃排空延迟。实验3中,正常蛋白饲料喂养4h的大鼠置于7.6%O_2缺氧环境中,直到缺氧24h后,缺氧大鼠的尿素排泄量与常氧大鼠无明显差异。而正常蛋白饮食喂养4h的大鼠，由于10.5%O_2缺氧，导致胃排空延迟。实验4中,高蛋白饲料喂养4h的大鼠置于7.6%O_2或10.5%O_2缺氧中,7.6%O_2缺氧大鼠血浆胃泌素浓度在缺氧6h后是常氧大鼠的2.3倍。缺氧6h期间，10.5%O_2低氧组与常氧组胃泌素水平无差异。此外，当禁食大鼠处于7.6%O_2或10.5%O_2缺氧时，缺氧3h后，两个缺氧组的胃泌素浓度均高于常氧组。实验5将幽门结扎后禁食大鼠置于7.6%O_2或10.5%O_2缺氧环境中3h。缺氧都会抑制胃酸的分泌并刺激胃泌素的释放。实验6中，幽门结扎大鼠禁食，口服HC1，然后置于7.6%O_2缺氧3h。口服HC1完全抑制了缺氧引起的胃泌素浓度升高。这些结果表明，缺氧抑制胃排空和分泌，并且缺氧对胃酸分泌的抑制作用通过正反馈调节刺激胃泌素释放。

项目成果

山地亮一: "低酸素条件下でラットを生育すると、胃排出及び胃酸分泌遅延を引き起こす" アメリカ栄養学会誌(J.Natr.). 126. 673-680 (1996)

Ryoichi Yamaji：“在缺氧条件下生长的大鼠会导致胃排空和胃酸分泌延迟”，《美国营养学会杂志》(J.Natr.) 126. 673-680 (1996)。

RYOICHI YAMAJI: "Hypoxia Inhibits Gastric Emptying and Gastric Acid secretion in Conscious Rats" J.Nutr. 126. 673-680 (1996)

RYOICHI YAMAJI：“缺氧抑制清醒大鼠的胃排空和胃酸分泌”J.Nutr。

Ryoichi Yamaji: "Hypoxia Inhibits Gastric Emptying and Gastric Acid Secretion in Conscious Rats" Journal of Nutrition. 126. 673-680 (1996)

Ryoichi Yamaji：“缺氧抑制清醒大鼠的胃排空和胃酸分泌”营养学杂志。