How does a plant express its secondary metabolism in the special tissues?

植物如何在特殊组织中表达其次生代谢？

基本信息

批准号：
05660120
负责人：
FUKUI Hiroshi
金额：
$ 1.34万
依托单位：
Dept.of Bioresource Science, Faculty of Agriculture, Kagawa University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1993
资助国家：
日本
起止时间：
1993 至 1994
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05660120/
关键词：
anthocyanin secondary metabolism regulation Raphanus sativus pigment protein Raphanus safivus 生合成誘導

项目摘要

More than 90% of useful plant metabolites such as medicines, flavors, pigments, food additives etc consumed in Japan have been imported from developing Southeast Asia. In addition, most of these plant metabolites are produced by wild plants. So, stable supply of these metabolites are anxious owing to the decrease of the plant-growing field. The supply should be done by an alternative method such as plant cell cultures. However, most of the useful metabolites can not be produced by the undifferentiated cultured cells ; regulation mechanisms of the plant secondary metabolisms that are formed and accumulated only in a peculiar tissues of plant have not been uncovered yet.Raphanus sativus produces anthocyanins, a red-colored secondary metabolite, in tissues or organs different from cultivar to cultivar. "KOSHIN" radish produces the red pigments inside the underground ; "AKAMARU" radish accumulates them in the outside of the tissue. indicating that this plant is appropriate for studying the regulation mechanism of plant secondary metabolism.The 4 days-old seedlings grown inthe dark start to produce the red pigments 24hr after being moved into the light. The illumination induces anthocyanin formation through yet unknown mechanism. In this study, proteins in the intact seedlings were investigated by 2-D electrophoresis (1 : NEpHGE,2 : SDS-PAGE). An alkaline protein of about 20kDA was found to appear 12hrs before formation of anthocyanin and disappear after the pigment accumulation. This protein might be an enzyme of anthocyanin formation or a regulating enzyme. The peptide sequence was not detected due probably to the derivatization of the N-terminal.

日本消费的药物、香料、色素、食品添加剂等有用植物代谢物90%以上是从东南亚发展中国家进口的。此外，这些植物代谢物大部分是由野生植物产生的。因此，由于植物种植面积的减少，这些代谢物的稳定供应变得令人担忧。应通过植物细胞培养等替代方法进行供应。然而，大多数有用的代谢物不能由未分化的培养细胞产生；仅在植物特定组织中形成和积累的植物次生代谢的调节机制尚未被揭示。萝卜在不同品种的组织或器官中产生花青素，一种红色次生代谢产物。 “KOSHIN”萝卜在地下产生红色素； “AKAMARU”萝卜将它们积聚在组织外部。表明该植物适合研究植物次生代谢的调控机制。暗处生长4天的幼苗移至光照下24小时后开始产生红色素。光照通过未知的机制诱导花青素的形成。在本研究中，通过二维电泳（1：NEpHGE，2：SDS-PAGE）研究了完整幼苗中的蛋白质。发现约20kDA的碱性蛋白质在花青素形成前12小时出现，并在色素积累后消失。该蛋白质可能是花青素形成的酶或调节酶。未检测到肽序列可能是由于 N 末端的衍生化。