Morphological study on nitiric oxide and soluble guanylate cyclases

一氧化氮和可溶性鸟苷酸环化酶的形态学研究

基本信息

批准号：
05454661
负责人：
FURUYAMA Shinobu
金额：
$ 4.93万
依托单位：
Osaka University (1994-1995)Osaka City University (1993)
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1993
资助国家：
日本
起止时间：
1993 至 1995
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05454661/
关键词：
soluble guanylate cyclase mitric oxide cGMP-dependent kinase isozymes subunits brain in situ hvbridization mRNA cGMP-dependent protein kinase α2サブユニット cyclicGMP‐dependent protein kinase 一酸化窒素 NO synathase 脳

项目摘要

Nitric oxide (NO) is an important intercellular messenger in the vessels and nervous system. NO activate guanylate cyclase to produce cGMP.Endothelial cells contain both NO and soluble guanylate cyclase (sGC) which exists as a heterodimer, while it is uncertain whether neurons contain both NO and soluble guanylate cyclase. First, using in situ hybridization we identified the localization of mRNA for alpha1 and beta1 subunits of sGC in the brain. Both alpha1 and beta1 subunits was expressed widely but unevenly in the brain. Strong expressions for both subunits were detected in many regions such as the neostriatum, accumbens nucleus, olfactory tubercle, and locus coeruleus These regions are suggested to contain active sGC as a target for NO.On the other hand, several regions did express no or only weak signals for alpha1 but expressed strong signals for beta1 subunits, suggested the presence of other alpha subunits in therse regions. Second, since little is known about receptor proteins … More for cGMP in the nervous system. cGMP-dependent protein kinase (GK) is one of main intracellular receptors for cGMP.Two types of GKs exists in vertebrate cells, a type 1 and type 2 form. The type 1 GK (GK1) exists as a homodimer and is generally extracted from soluble fraction of various tissues, while the type 2 GK (GK2) is a membrane-bound monomer. GK1 is widely distributed and predominant form in many tissues of vertebrate. Despite the abundant expression of GK1 in smooth muscle and many periphery tissues, in the brain its presence has been reported only in the Purkinje cells of the cerebellum by using immunohistochemistry. In the present sudy we displayd distinct distribution of GK1 and GD2, and more extensive distribution for GK1and GK2. Strong expressions of GK1 in the hippocampus and cerebellum suggest a role for this kinase in mediating the long-term potentiation and long-term depression of NO via activation of sGC in these regions. On the other hand, strong expressions of GK2 in the thalamus support a role for the kinase in mediating another actions of NO there. The wide distributions of GK1 and GK2 in the brain suggests that cGMP-mediated protein phosphorylation occurs in many brain regions. Less

一氧化氧化物（NO）在血管中和神经系统中的重要细胞中心。在大脑中的alpha1和beta1 sugc的mRNA。包含VE SGC作为No.ONE的目标，严重性区域确实没有或仅表示Alpha1的弱信号，但对Beta1亚基表示强大的信号，这是CGMP在CGMP中的Alpha亚基的存在。系统在脊椎动物的许多组织中是广泛分布的，并且在大脑中，它的存在仅在小脑的Purkinje细胞中才能通过免疫组化。 GD2，以及在海马和小脑中GK1的更广泛的分布g表明激酶在介导SGC中NOA激活的长期潜力和长期深度中的作用，另一方面是强大的。 GK2在培养基中的角色中的另一个作用