Enzyme-histochemical and three-dimensional reconstruction study of lymphatic vessels in the dental pulp.

牙髓淋巴管的酶组织化学和三维重建研究。

• 批准号: 05454485
• 负责人: YOSHIDA Shigemitsu
• 金额: $ 4.22万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05454485/
• 关键词: Dental pulp; Lymphatic vessel; Enzyme-histochemistry; 5'-nucleotidase; Alkaline phosphatase; Three-dimensional reconstruction; ヌクレオチダーゼ

To clarify the distribution and three-dimensional architecture of the lymphatic vessels in the dental pulp, human molar teeth were examined using an enzyme-histochemical method (Kato, 1989 ; 5'-nucleotidase (5'-Nase) -Alkaline phosphatase (ALPase) double staining method). The results are summarized as follows :1. Existence of lymphatic vessels in the human dental pulp was confirmed under light microscope, scanning electron microscope, and transmission electron microscope. The 5'-Nase-Alpase double staining method is useful for studying the fine distribution and three-dimensional architecture of the lymphatic vessels in the human dental pulp.2. This double staining method was applicable for both frozen sections and JB-4 embedded sections, however, 5'-Nase activity disappeared after decalcification.3. Thick lymphatic vessels were located mainly in the center of the pulp and thin vessels were found both in the center and periphery of the pulp, which means that the lymphatics in the dental pulp originated from wide areas of the pulp. However, lymphatic vessels were not observed in the odontoblast layr.4. Three-dimensional reconstruction study revealed that the lymphatic vessels exhibit both dilations and constrictions along their length, and showed that the ramification patterns of the lymphatics were different from those of the blood vessels.5. The number and distribution of the lymphatic vessels in the dental pulp showed considerable individual variations.

为了阐明牙髓中淋巴管的分布和三维结构，使用酶组织化学方法检查了人磨牙（Kato，1989；5'-核苷酸酶（5'-Nase）-碱性磷酸酶（ALPase）双染色法）。研究结果如下： 1．在光学显微镜、扫描电子显微镜和透射电子显微镜下证实人牙髓中存在淋巴管。 5'-Nase-Alpase双染法可用于研究人牙髓淋巴管的精细分布和三维结构。 2．该双重染色方法适用于冰冻切片和JB-4包埋切片，但脱钙后5'-Nase活性消失。 3．粗的淋巴管主要分布在牙髓的中心，细的血管分布在牙髓的中心和周边，这表明牙髓中的淋巴管起源于牙髓的广泛区域。但成牙本质细胞层未观察到淋巴管。4．三维重建研究表明，淋巴管沿长度方向同时存在扩张和收缩现象，并表明淋巴管的分支模式与血管不同。 5．牙髓中淋巴管的数量和分布表现出相当大的个体差异。

项目成果

Aoyama, Y., Yoshida, S.et al: "Lymphatic vessels in the human dental pulp : An enzyme-histochemical study." Japanese Journal of Oral Biology. 37 (Submitting). (1995)

Aoyama, Y., Yoshida, S.等人：“人牙髓中的淋巴管：一项酶组织化学研究。”

青山洋子、吉田重光 他3名: "ヒト歯髄のリンパ管に関する酵素組織化学的研究" 歯科基礎医学会雑誌. 37(投稿中). (1995)

Yoko Aoyama、Shigemitsu Yoshida 等 3 人：“人牙髓淋巴管的酶组织化学研究”日本基础牙科医学会杂志 37（目前正在提交）。