Immuno-histochemical and microbiological study on the relationships between microcirculatory system and immuno-defence system in the dental pulp.

牙髓微循环系统与免疫防御系统关系的免疫组织化学和微生物学研究。

基本信息

批准号：
09470389
负责人：
YOSHIDA Shigemitsu
金额：
$ 8.64万
依托单位：
HOKKAIDO UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B).
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 2000
项目状态：
已结题

项目摘要

To clarify the relationships between microcirculatory system and immuno-defence system in the dental pulp, we examined human blood and lymphatic vessels immuno-histochemically and microbiologically. The results are summarized as follows :1)In general, lymphatic vessels in healthy tissue express PECAM-1 only, but in inflamed tissue, they express PECAM-1, ICAM-1, ICAM-3, and VCAM-1.2) In the submandibular lymph node, lymphatic vessels express PECAM-1 and ICAM-1.3) In the tongue, lymphatic capillaries close to oral epithelitum express PECAM-1, ICAM-1, and ELAM-1, or PECAM-1 and ICAM-1. However, collecting lymphatic vessels express PECAM-1 only.4) Mediation of inflammatory cytokines induce strong expression of PECAM-1, and expression of ICAM-1 on the lymphatic vessels.5) Some oral microbial associates induce expression of leukocyte adhesion molecules and production of cytokines in the vascular endothelial cells.6) In blood vessels of healthy dental pulp, there are strong expression of PECAM-1 and weak expression of MHC class II, ELAM-1, and ICAM-1.7) In blood vessels of inflamed dental pulp, there are strong expression of PFCAM-1, MHC class II, ELAM-1, ICAM-1, ICAM-3, and VCAM-1.8) In lymphatic vessels of healthy and inflamed dental pulp, there are strong expression of PECAM-1 and weak expression of ICAM-1.9) PECAM-1 expression of the blood vessels was observed only on the luminal surface of the endothelium, but in the lymphatic vessels, it was found both on the luminal and abluminal surfaces of the endothelium.10) Expression of desmoplakin is observed only in the lymphatic vessels, but not in the blood vessels, indicating that anti-desmoplakin antibody is useful to discriminate lymphatic vessels from blood vessels.

为了阐明牙髓微循环系统和免疫防御系统之间的关系，我们对人体血液和淋巴管进行了免疫组织化学和微生物学检查。结果总结如下：1）一般情况下，健康组织中的淋巴管仅表达PECAM-1，但在发炎组织中，它们表达PECAM-1、ICAM-1、ICAM-3和VCAM-1.2）在颌下淋巴结、淋巴管表达 PECAM-1 和 ICAM-1.3) 在舌头中，靠近口腔上皮的毛细淋巴管表达 PECAM-1、ICAM-1 和ELAM-1，或 PECAM-1 和 ICAM-1。然而，集合淋巴管仅表达 PECAM-1。4) 炎症细胞因子的介导诱导 PECAM-1 的强烈表达，以及淋巴管上 ICAM-1 的表达。5) 一些口腔微生物伙伴诱导白细胞粘附分子的表达和产生6）健康牙髓血管中，PECAM-1强表达，MHC II类、ELAM-1、 ICAM-1.7) 在发炎牙髓的血管中，PFCAM-1、MHC II 类、ELAM-1、ICAM-1、ICAM-3 和 VCAM-1.8) 在健康和发炎牙的淋巴管中强烈表达牙髓中 PECAM-1 强表达，ICAM-1.9 弱表达）血管中 PECAM-1 表达仅在内皮管腔表面观察到，但在10) 仅在淋巴管中观察到桥粒斑蛋白表达，而在血管中未观察到，表明抗桥粒斑蛋白抗体可用于区分淋巴管和淋巴管。血管。