TRANSCRIPTIONAL REGULATION DURING MAMMALIAN NEUROGENESIS

哺乳动物神经发生过程中的转录调控

• 批准号: 04454002
• 负责人: TAKAHASHI Naoki
• 金额: $ 3.9万
• 依托单位: NARA INSTITUTE OF SCIENCE AND TECHNOLOGY (1994)Osaka University (1993)Nagoya University (1992)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04454002/
• 关键词: GENE; NERVOUS SYSTEM; DEVELOPMENT; TRANSCRIPTIONAL REGULATION; PCR; MOLECULAR BIOLOGY

The homeobox is a 183-base-pare DNA sequence originally found in Drosophila segmentation and homeotic genes. InDrosophila, homeotic genes are clustered in the Antennapedia and Bithorax complexes, collectively called the homeotic gene coplex (HOM-C). In the mouse genome, about 40 homeobox genes (Hox) are clustered in four chromosomal regions (Hox A to D). The Hox genes are arranged in the same order and have the same antero-posterior patten of expression as thier structural homologue in the HOM-C,suggesting that they control mouse pattern formation in the same way that HOM-C members do in Drosophila. Homeobox gene products are believed to be transcription factors that regulate expression of target genes. A few candidate target genes have been identified in Drosophila by various approaches but the Hox gene targets are poorly understood, mostly because of limitations in the available approaches. In this research project we identify several candidate Hox gene targets, including a mouse homologue if the Drosophila tumor-suppressor gene l (2) gl, by immunopurification of DNA sequences bound to a Hox protein in native chromatin.

同源物是最初在果蝇分割和同型基因中发现的183碱基-PARE DNA序列。嗜嗜性酚类，同源基因聚集在抗植物和Bithorax复合物中，共同称为同源基因Coplex（HOM-C）。在小鼠基因组中，大约40个同源基因基因（HOX）聚集在四个染色体区域（HOX A至D）中。 HOX基因以相同的顺序排列，并且具有与HOM-C中的THIR结构同源物相同的前后表达patten，这表明它们以与HOM-C成员在果蝇中相同的方式控制小鼠模式的形成。据信同源基因基因产物是调节靶基因表达的转录因子。通过各种方法在果蝇中鉴定出了一些候选靶基因，但是HOX基因靶标的理解很少，这主要是由于可用方法的局限性。在该研究项目中，我们确定了几个候选HOX基因靶标，包括小鼠同源物，如果果蝇肿瘤 - 抑制基因L（2）GL（通过对与天然染色质中HOX蛋白结合的DNA序列结合的DNA序列）的免疫性化。

项目成果

Avraham,K.et al.: "Murine chromosomal locations of four class III POU transcription factors." Genomics. 18. 131-133 (1993)

Avraham,K.et al.：“四种 III 类 POU 转录因子的小鼠染色体位置。”

高橋 直樹 他(日本生化学会編): "新生化学実験講座14,発生,分化,老化" 東京化学同人, 541 (1992)

高桥直树等（日本生物化学会编）：《新生物化学实验课程14，发育、分化、老化》东京化学同人，541（1992）

Goto, J., Miyabayashi, T., Wakamatsu, Y., Takahashi, N.and Muramatsu, M.: "Organization and expression of mouse Hox 3 cluster genes." Mol.Gen.Genet.239. 41-48 (1993)

Goto, J.、Miyabayashi, T.、Wakamatsu, Y.、Takahashi, N. 和 Muramatsu, M.：“小鼠 Hox 3 簇基因的组织和表达。”

Kuwabara,K.et.al: "mgl-1,a mouse homologue of the Drosophila tumor-suppressor gene e(2)gl,maps to chromosome 11." Genomics. 20. 337-338 (1994)

Kuwabara,K.et.al：“mgl-1，果蝇肿瘤抑制基因 e(2)gl 的小鼠同源物，映射到 11 号染色体。”

Suemori,H.et.al.: "Disruption of the HoxC-9 gene results in anterior trausformation in the vertebrae and malformation in the sternum and ribs" Mech.Dev.(in press). (1995)

Suemori,H.et.al.：“HoxC-9 基因的破坏导致椎骨前部变形以及胸骨和肋骨畸形”Mech.Dev.（印刷中）。