Inverted laser scanning microscope

倒置激光扫描显微镜

• 批准号: 430680185
• 金额: --
• 依托单位: Medizinische Hochschule Hannover (MHH)
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2019
• 资助国家: 德国
• 起止时间: 2018-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/430680185?language=en
• 关键词: Inverted; laser; scanning; microscope

We apply for funding of a microscope with confocal scan had as replacement for our currently used system. Recent advances and new developments in confocal microscopy have led to a quantum leap-like improvement of the instruments over the last few years. Therefore, users of our Central Research Facility for Laser Microscopy are demanding confocal microscopes that go well beyond the capabilities of the first generation of the facility's instrumentation. This is essential, for example, for the quantitative analysis of intracellular Ca2+ signals using fluorescence-based indicator dyes. Particularly important is the increased sensitivity and the significantly improved signal-to-noise ratio of the new detector generation, which makes it possible to measure weak signals at maximum scanning speed over the entire field of view in a reproducible manner. Further measures in expanding the sensitivity of modern microscopy systems expand their application range towards physiological measurements. A large number of projects address dynamic processes such as changes during cell movement, mitosis, cytokine synthesis, endocytosis and changes in the interaction between pathogens and host cells. In these applications, it is of utmost importance for the users to achieve the best possible resolution with minimum photon exposure.

我们申请使用共聚焦扫描的显微镜资金，以替代我们当前使用的系统。在过去的几年中，最近的进展和共聚焦显微镜的新发展导致了工具的量子飞跃改进。因此，我们的激光显微镜中央研究设施的用户要求共聚焦显微镜，远远超出了该设施仪器第一代的能力。例如，对于使用基于荧光的指示剂染料对细胞内CA2+信号进行定量分析是必不可少的。特别重要的是，新检测器生成的灵敏度提高和明显提高的信噪比，这使得可以以可重复的方式在整个视野上以最大扫描速度测量弱信号。扩大现代显微镜系统敏感性的进一步措施将其应用范围扩展到生理测量。许多项目解决了动态过程，例如细胞运动，有丝分裂，细胞因子合成，内吞作用以及病原体与宿主细胞之间相互作用的变化。在这些应用程序中，对于用户来说，通过最低光子暴露实现最佳分辨率至关重要。