Inverted Confocal Laser Scanning Microscope

倒置共焦激光扫描显微镜

• 批准号: 441730715
• 金额: --
• 依托单位: Friedrich-Alexander-Universität Erlangen-Nürnberg
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2020
• 资助国家: 德国
• 起止时间: 2019-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/441730715?language=en
• 关键词: Inverted; Confocal; Laser; Scanning; Microscope

In this proposal we apply for the replacement of a confocal laser scanning microscope, which now is in use for more than 10 years. More than 40 research groups made use of the instrument. The present instrument served 41 PhD thesises, more than 100 conference contributions and at least 35 peer reviewed publications. Systematic monitoring of publications only was performed within the last four years, which means the outcome of publications is likely to be much higher.The replacement instrument will be used on different scientific backgrounds such as medical, natural science (anima, planta) and material science. Independend from the plethora of individual extremly versatile backgrounds a few common requirements are linked to the majority of all proposed research. The instrument will be used for multi-channel (3 -4 in average) image acquisition of fixed samples (cells, tissue, crystalline surfaces) in 2 and 3 dimensions. The instrument must deliver high-resolution down to Abbe's limitation, but no super resolution. Emission technology must enable to fine tune the detection range of various emission spectra, even from within the same fluorescent dye changing behaviour under different conditions. A mixture of conventional photomultiplying tubes (PMT) and high sensitive detectors. Excitation in the visible spectrum at all major standard fluorescent excitation labels must be possible. Big physical field of view, in particular for imaging tissues and surfaces with less tiles and hence less stitching to apply. Further a high precision auto focus must be in place for best threedimenional (z-stack) imaging. All these requires a fully automated microscope stage.

在此提案中，我们申请替换共聚焦激光扫描显微镜，该扫描显微镜现在已经使用了10年以上。超过40个研究小组利用了该工具。本工具提供了41位博士学位论文，100多个会议贡献和至少35个同行评审出版物。仅在过去四年内对出版物进行系统监控，这意味着出版物的结果可能更高。替代工具将用于不同的科学背景，例如医学，自然科学（Anima，Planta）和材料科学。独立于大量的极端用途背景，有一些共同的要求与所有拟议的研究中的大多数有关。该仪器将用于2和3维度的固定样品（细胞，组织，晶体表面）的多通道（平均3-4）图像采集。该仪器必须将高分辨率降低到Abbe的限制，但没有超级分辨率。排放技术必须使能够微调各种发射光谱的检测范围，即使在不同条件下相同的荧光染料改变行为中也是如此。常规光电管（PMT）和高灵敏探测器的混合物。在所有主要标准荧光激发标签上，可见光谱中的激发是可能的。较大的物理视野，特别是用于成像瓷砖较少的组织和表面，因此缝合较少。进一步，必须建立高精度的自动焦点，才能获得最佳的三二聚体（Z-stack）成像。所有这些都需要一个全自动显微镜阶段。