The roles of gammadelta T cells in host defense aginst bacterial infection

γδ T 细胞在宿主防御细菌感染中的作用

• 批准号: 02454191
• 负责人: YOSHIKAI Yasunobu
• 金额: $ 4.29万
• 依托单位: Nagoya University (1991-1992)Kyushu University (1990)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1990
• 资助国家: 日本
• 起止时间: 1990 至 1992
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-02454191/
• 关键词: Salmonella; Listeria; Mycobacteria; gammadeltaTcells; 65kdHSP; Cytokine; Host defense; 感染防御; ビルレンス; Ity遺伝子; HSP60; γδT細胞; 大腸菌

T cells recognize nominal antigens in the context of self-MHC antigens by T cell receptor(TcR) which is composed of alpha and beta chains. Another type of TcR, which is composed of gamma and delta chains has also identified. At least a significant fraction of gamma/delta T cells are reported to be specialized to recognize epitopes on mycobacterial antigens, suggesting that the gamma/delta bearing T cells may participate in immune surveillance against invasion with the various pathogens.To elucidate a potential role of the gamma/delta T cells in host-defense against bacterial infection,we examined the kinetics of gamma/delta T cells and their ligands during intraperitoneal infections with Listeria monocytogenes, Salmonella chorelaesuis and BCG.During infection with such intracellular bacteria, TcR gamma/delta T cells specific for 65kd hsp precede TcR alpha/beta T cells specific for bacterial antigens in appearance. The gamma/delta T cells provide a first line of defense against the infection by recognizing exogenous and endogenous 65 kd hsp on infected cells and producing cytokines such as gammaIFN. Our results suggest that the hsp- specific gamma/delta T cells respond quickly to antigenically diverse pathogens before antigen-specific alpha/beta T cells expand clonally, and they play a role in covering the gap between the phagocytic system and highly evolved immune response mediated by alpha/beta T cells.

T细胞在由α和β链组成的T细胞受体（TCR）的自我MHC抗原的背景下识别名义抗原。也确定了由伽马和三角洲链组成的另一种类型的TCR。据报道，至少有很大一部分的γ/delta T细胞专门识别分枝杆菌抗原的表位，这表明γ/delta轴承T细胞可能参与免疫监测，以防止对各种病原体的入侵进行侵袭。在腹膜内感染单核细胞增生李斯特菌，沙门氏菌切氏菌和BCG的过程中，γ/delta T细胞及其配体在这种细胞内细菌，TCRγ/delta T细胞中的感染。通过识别受感染细胞的外源和内源性65 kD HSP并产生诸如γFN等细胞因子的外源和内源性65 kD HSP，伽马/三角洲T细胞通过识别外源和内源性65 kD HSP提供了对感染的第一道防线。我们的结果表明，在抗原特异性的α/βT细胞迅速对抗原多样的病原体反应中，HSP-特异性γ/delta T细胞反应迅速，并且它们在覆盖吞噬系统和高度演化的免疫反应之间的间隙中发挥作用，并由Alpha/beta T细胞介导的高度演化的免疫反应。

项目成果

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Emoto,M.,et al.: "Different appearance of γδ T cells during salmonellosis between Ity^r and Ity^s mice." J.Immunol.(1993)

Emoto, M., et al.：“Ity^r 和 Ity^s 小鼠沙门氏菌病期间 γδ T 细胞的不同表现。J.Immunol。”(1993)

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Yasunobu Yoshikai：“γδT细胞和疾病 - 结核病” Kokusai Isho Publishing，6（1990）

Inoue,T.,et al.: "Early appearing γδ-bearing T cells during infectin with Calmette Guerin Bacillus." J.Immunol.146. 2754-2762 (1991)

Inoue, T., et al.：“卡介苗感染过程中早期出现的 γδ 携带 T 细胞。J.Immunol.146 (1991)。

Tauchi,Y.,et al.: "A new subpopulation of intestinal intraepithelial lymphocytes expressing high level of TcRγδ recognize IE^<k.>" Eur.J.Immunol.22. 2465-2468 (1992)

Tauchi，Y.，等人：“表达高水平 TcRγδ 的肠上皮内淋巴细胞的新亚群识别 IE^<k.>”Eur.J.Immunol.22（1992）。