Molecular mechanisms for generation and maintenance of memory CD8T cells following bacterial infection.

细菌感染后记忆 CD8T 细胞生成和维持的分子机制。

• 批准号: 13226036
• 负责人: YOSHIKAI Yasunobu
• 金额: $ 38.78万
• 依托单位: Kyushu University (2002-2005)Nagoya University (2001)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research on Priority Areas
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-13226036/
• 关键词: IL-15; memory CD8 T cells; apoptosis; Listeria; homeostatic proliferation; reactivation; 再感染; 2次免疫応答; グランザイム; 細胞分裂; 感染防御; 細胞傷害活性; 肝傷害; IL-15トランスジェニックマウス; IL-15ノックアウトマウス; Listeria monocytogene; BCG; Listeria monocytogeness; エフェクターT細胞; メモ

During the course of acute infection with an intracellular pathogen, antigen-specific T cells proliferate in the expansion phase, and then most of the T cells die by apoptosis in the following contraction phase, but the few that survive become memory cells and persist for a long period of time. Memory CD8T cells are activated rapidly against re-infection and eliminate pathogens. Using an adoptive transfer system of OT-I cells expressing OVA_<257-264>/K^b-specific T cell receptor into control, IL-15 knockout (KO) and IL-15 transgenic (Tg) mice followed by challenge with recombinant Listeria monocytogenes expressing OVA, we found the roles of IL-15 in generation, maintenance and reactivation of memory CD8T cells as follows :(1) Effector phase : IL-15 is dispensable for generation of effector CD8T cells following bacterial infection.(2) Contraction phase : IL-15 plays a critical role in protecting effector CD8^+T cells from apoptosis during the contraction phase following a microbial infection via inducing anti-apoptotic molecules.(3) Maintenance phase : IL-15 plays an important role in long-term maintenance of memory CD8^+ T cells through induction of homeostatic proliferation.(4) Reactivation : IL-15 plays an important role in early activation of memory CD8^+ T cells through induction of cytotoxic molecules upon re-infection.

在细胞内病原体急性感染过程中，抗原特异性T细胞在膨胀阶段增殖，然后大多数T细胞在以下收缩阶段因凋亡而死亡，但生存的少数人会成为记忆细胞并持续很长一段时间。记忆CD8T细胞因重新感染而迅速激活并消除病原体。 Using an adoptive transfer system of OT-I cells expressing OVA_<257-264>/K^b-specific T cell receptor into control, IL-15 knockout (KO) and IL-15 transgenic (Tg) mice followed by challenge with recombinant Listeria monocytogenes expressing OVA, we found the roles of IL-15 in generation, maintenance and reactivation of memory CD8T cells as follows :(1)效应子阶段：IL-15对于细菌感染后的效应CD8T细胞的产生是可分配的。（2）收缩阶段：IL-15在保护效应的CD8^+ T细胞在接收阶段免于凋亡在微生物感染期间的凋亡阶段至关重要。 （4）重新激活：IL-15在重新感染后通过诱导细胞毒性分子的诱导，在记忆CD8^+ T细胞的早期激活中起重要作用。

项目成果

シンプル免疫学(中島泉, 高橋利忠, 吉開泰信共著)「感染に対する免疫 免疫の障害 : 免疫不全症」

简单免疫学（中岛泉、高桥利忠、吉凯康信合着）“感染免疫：免疫疾病：免疫缺陷疾病”

• 发表时间: 2004
• 作者: 野村 卓正;光山正雄;光山正雄;吉開泰信;吉開泰信
• 通讯作者: 吉開泰信

Interleukin-15 prevents allergic rhinitis though activation of antigen-specific CD8^+T cell.

Interleukin-15 通过激活抗原特异性 CD8^ T 细胞来预防过敏性鼻炎。

• 发表时间: 2006
• 期刊: J.Allergy Clin.Immunol. (In press)
• 作者: Aoi N.;et al.
• 通讯作者: et al.

現代医療33「Innate immunity の分子基盤」

现代医学33《先天免疫的分子基础》

• 发表时间: 2001
• 作者: 菊池 毅;松口 徹也;吉開 泰信;吉開 泰信
• 通讯作者: 吉開 泰信

MyD88-dependent signaling for IL-15 production plays an important role in CD8aa TCRa4 and TCRy8 intestinal intraepithelial lymphocytes

MyD88 依赖性 IL-15 产生信号在 CD8aa TCRa4 和 TCRy8 肠上皮内淋巴细胞中发挥重要作用

• 发表时间: 2006
• 期刊: J.Immunol. 176
• 作者: Yu Q.;Tang C.;Xun S.;Yajima T.;Takeda K.;Yoshikai Y.
• 通讯作者: Yoshikai Y.

ウイルス・細胞と感染症がわかる(吉開泰信編)「感染症の発症機構-病原体と宿主の攻防」、「微生物感染とたたかう生体防御機構」

了解病毒、细胞和传染病（吉海康信主编）《传染病的发病机制——病原体与宿主之间的战斗与防御》、《对抗微生物感染的生物防御机制》

• 发表时间: 2004
• 作者: 吉開 泰信;久保 千春;吉開 泰信;吉開 泰信
• 通讯作者: 吉開 泰信