Pathophysiology and clinical meaning of physiologic cholestasis

生理性胆汁淤积的病理生理学和临床意义

基本信息

批准号：
01480259
负责人：
SHIRAKI Kazuo
金额：
$ 4.16万
依托单位：
Tottori University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1989
资助国家：
日本
起止时间：
1989 至 1991
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-01480259/
关键词：
Physiologic cholestasis Vitamin K deficiency Breast milk jaundice Neonatal hepatitis syndrome Breast milk Primary cultured hepatocyte Epidermal growth factor 新生児肝炎症候群初代培養肝細胞初代培養肝細胞系 hepatocyte growth factor 母乳母乳性遷延性黄疸胆汁うっ滞

项目摘要

We studied the cholestasis which physiologically seen in many normal infants. The cholestasis was ordinarily seen in neonatal through infantile period, , and that reached the top level at late neonatal period of 4 weeks after birth.We found that the diseases of infantile vitamin K deficiency, breast milk jaundice and neonatal hepatitis syndrome were occured mainly based on insufficiency of bile acid secretion into the intestine. These diseases were usually seen in breast-fed infants, so that we studied the effect of human milk on liver function, especially DNA synthesis in primary cultured neonatal rat he atocytes. Isolated hepatocytes in 5-oay old neonatal rats were cultured in a medium with human milk, or with controls. DNA syntheses of hepatocytes were measured by incorporation on P H)thysidine. Human milk promoted DNA synthesis in neonatal rat hepatocytes more than that of controls. The sitogenic activity of human milk shoved no correlation with the concentration of, human epidernal growth factor(HEGF). Anti-HEGF antibody did not inhibit the sitogenic activity completely. These results suggest the presense of sitogens other than HEGF in human milk.From, our results, clinical course of physiologic cholestasis was elucidated. Hovever some problems, espegi 11 the association between the cholestasis and breast feeding, remains to be solved.

我们研究了许多正常婴儿生理上观察到的胆汁淤积。新生儿期胆汁淤积多见于婴儿期，以出生后4周的新生儿后期达到最高水平。我们发现婴儿维生素K缺乏症、母乳性黄疸、新生儿肝炎综合征等疾病的发生主要基于以下因素：肠道内胆汁酸分泌不足。这些疾病多见于母乳喂养的婴儿，因此我们研究了母乳对原代培养的新生大鼠肝细胞肝功能，特别是DNA合成的影响。将 5 岁新生大鼠的分离肝细胞培养在含有人乳或对照的培养基中。通过掺入P(H)胸苷来测量肝细胞的DNA合成。母乳对新生大鼠肝细胞 DNA 合成的促进作用高于对照组。母乳的促生长活性与人表皮生长因子（HEGF）的浓度没有相关性。抗HEGF抗体没有完全抑制位点活性。这些结果表明人乳中存在除 HEGF 之外的其他物质。根据我们的结果，阐明了生理性胆汁淤积的临床过程。然而，espegi 11 胆汁淤积与母乳喂养之间的关系仍有待解决。