Development of High Quarity of Chemical Sensors Based on Polymeric Thin Film Coating

基于聚合物薄膜涂层的高质量化学传感器的开发

基本信息

批准号：
01470064
负责人：
OYAMA Noboru
金额：
$ 3.52万
依托单位：
Tokyo University of Agriculture and Technology
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1989
资助国家：
日本
起止时间：
1989 至 1991
项目状态：
已结题

项目摘要

Polymer coated electrodes (PCEs), which consist of a metallic or carbon conductor direct coated with an ion-sensitive, electrocatalytic, or enzyme membrane, are very attractive potentiometric and amperometric sensors due to their simplicity of operation, superior amperometric response characteristics, capability of minimization, etc., compared with conventional membrane electrodes.In this research, new amperometric sensors for determination of glucose and cholesterol based on bilayer film coating have been fabricated and their sensor characteristics under a steady-state condition have been examined. The glucose or cholesterol concentration could be monitored by measuring the current for H_2O_2 or O_2 reduction electrocatalyzed inner film where H_2O_2 was produced orO_2. was consumed by the enzyme reaction in the outer enzyme films. The reproducible relationship between glucose or cholesterol concentration and sensor output was obtained. The PCEs functioned as an electrochemical detecto … More r for flow injection analysis. Substantial unprovement in the selectivity of arnperometric detection for flow injection system was observed as a result of an electrostatic interaction between the positively charged electropolymerized film and analysis, that is, cationic species (e. g., dopamine, 1-norepinephrine and DL-3, 4-dihydroxyphenylalanine) exhibited little response, while the response of neutral and anionic species (e. g., 3, 4-dihydroxyphenylacetic acid, 4-hydroxy-3-methoxyphenylacetic acid and catechol) was only slightly changed at the polymer coated electrode compared with the bare electrode.The complex admittance measurements of the quartz crystal oscillators with and without a film coating, which are in contact with air, moisture and supporting electrolyte aqueous media of various concentrations have been carried out over a frequency range covering the area of the resonant frequency of each oscillator system, and the results have been represented in the form of conductance and susceptance spectra (i. e., conductance vs. frequency plots and susceptance vs. frequency plots). According to the electrical equivalent circuit model conventionally proposed for a piezoelectric quartz crystal, the related circuit parameters (resistance R, inductance L, capacitance C and static capacitance C_0) have been evaluated, especially as a function of the concentration of supporting electrolyte. The change of electrolyte concentration-dependent swelling of the film was characterized by the evaluated parameters. It is demostrated that the method described can can apply the characterized kinematic viscosity and affinity sensing of the organic, inorganic and biological thin films. The quartz crystal microbalance (QCM) when operated in liquids was shown to retain its high mass sensitivity. The QCM has therefore been applied to the mass changes occurring during redox in thin polymer films. Solvent transport during electroneutrality requiring ion transport has been qualitatively demonstrated in the case of electrodes modified with thin film of poly (vinyferrocene), polyaniline, etc. Less

聚合物涂层电极 (PCE) 由直接涂有离子敏感膜、电催化膜或酶膜的金属或碳导体组成，由于其操作简单、卓越的电流响应特性、本研究制备了基于双层薄膜涂层的新型电流传感器，用于测定葡萄糖和胆固醇，其稳态条件下的传感器特性得到了改善。葡萄糖或胆固醇浓度可以通过测量H_2O_2或O_2还原电催化内膜的电流来监测，其中H_2O_2被外酶膜中的酶反应产生或消耗O_2。葡萄糖或胆固醇浓度与传感器输出之间的可重复关系为。 PCE 用作流动注射分析的电化学检测器，流动注射系统的安培检测的选择性尚未得到充分验证。观察到带正电的电聚合膜和分析之间的静电相互作用，即阳离子物质（例如多巴胺、1-去甲肾上腺素和DL-3,4-二羟基苯丙氨酸）表现出很少的响应，而中性和阴离子物质的响应种（例如，3, 4-二羟基苯乙酸、4-羟基-3-甲氧基苯乙酸和儿茶酚）与裸电极相比，聚合物涂层电极仅略有变化。对有或没有薄膜涂层的石英晶体振荡器与空气、水分和不同浓度的支持电解质水介质接触进行了复导纳测量在覆盖每个振荡器系统谐振频率区域的频率范围内，结果以电导谱和电纳谱的形式表示（即电导与频率图和根据通常提出的压电石英晶体的电气等效电路模型，评估了相关电路参数（电阻 R、电感 L、电容 C 和静态电容 C_0），特别是作为通过评估参数来表征电解质浓度依赖性薄膜膨胀的变化，这表明所描述的方法可以应用所表征的运动学。有机、无机和生物薄膜的粘度和亲和感测表明，石英晶体微天平 (QCM) 在液体中操作时可保持其高质量灵敏度，因此已应用于薄聚合物氧化还原过程中发生的质量变化。在用聚（乙烯基二茂铁）、聚苯胺等薄膜修饰的电极的情况下，已经定性地证明了需要离子传输的电中性过程中的溶剂传输。