Development of a new biotechnological manufacturing process of bifidogenic substances in milk

牛奶中双歧物质的新生物技术制造工艺的开发

• 批准号: 61860030
• 负责人: ADACHI Susumu
• 金额: $ 5.12万
• 依托单位: TOHOKU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Developmental Scientific Research
• 财政年份: 1986
• 资助国家: 日本
• 起止时间: 1986 至 1987
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-61860030/
• 关键词: Bifidus factor; Trichoderma harzianum; 6'-galactosyl-lactlose; ビフィダス因子; トリコデルマ; ビフィダス活性; 6'-ガラクトシル・ラクトース

Trichoderma harzianum produces galacto-oligosaccharides particularly tri- saccharides during growth in lactose containing medium. 6'-Galactosyl-lactgose is the main oligosaccharide formed by Trichoderma harzianum. In its active state, the <beta>-galactosidase isolated from Trichoderma harzianum shows signi- ficant transgalactosylation activity. The Trichoderma harzianum strain used in the presen tinvestigation is ghghly capable of forming <beta>(1-6) linkage which in turn leads to high amount production of 6'-galactlosyl-lactgose. It is thorght that the present strain of Trichoderma harzianum could be further studied critically to formulate optimum process conditions for use in large scale production of bifidogenic oligosaccharides particularly 6'-galactosyl- -lactose from lactose.

甲状腺素harzianum在含有乳糖培养培养基的生长过程中，尤其是三糖。 6'-半乳糖基 - 乳糖是由Trichoderma harzianum形成的主要寡糖。在其活性状态下，从甲状腺素harzianum分离出的<beta> - 半乳糖苷酶显示出具有特征性的经乳糖基化活性。 tinVestigation中使用的毛霉素harzianum菌株具有形成<beta>（1-6）链接的ghghly，进而导致高量产生6' - 甲状腺乳糖基 - 乳糖。索尔格特（Thorght）可以对目前的trichoderma harzianum的菌株进行批判性研究，以制定最佳过程条件，以在大规模生产分生成寡糖的大规模生产中，尤其是乳糖中的6'-半乳糖基 - 乳糖。

项目成果

Sai Prakash, Kyozo Suyama, Takatoshi Itoh, Susumu Adachi: "Oligoaccharide formation by Tgrichoderma harzianum in lactose containing medium." Biotechnology Letters. 9. 249-252 (1987)

Sai Prakash、Kyozo Suyama、Takatoshi Itoh、Susumu Adachi：“哈茨木霉在含乳糖培养基中形成寡糖。”

Sai Prakash B;Kyozo Suyama;Takatoshi Itoh;Susumu Adachi: Biotechnology.

Sai Prakash B；Kyozo Suyama；Takatoshi Itoh；Susumu Adachi：生物技术。

Sai Prakash; Kyojo Suyama; Takatoshi Itoh, and Susumu adachi: Biote chnolojy Letters. 9. 249-252 (1987)

赛·普拉卡什；