Preparative method of functional oligasaccharides from bovine colostrum

牛初乳功能性低聚糖的制备方法

• 批准号: 59860031
• 负责人: ADACHI Susumu
• 金额: $ 4.03万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Developmental Scientific Research
• 财政年份: 1984
• 资助国家: 日本
• 起止时间: 1984 至 1985
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-59860031/
• 关键词: caseinoglycopeptide; <kappa> -casein; bovine colostrum; oligosaccharide; ビフィドバクテリウム活性

Colostrum was taken from a Holstein-Friesian cow 6h after parturition. Isolation of milk sugars from defatted colostrum by dialysis was fermormed by the method of Schneir and Rafelson. The complete removal of peptides from the dialyzate was achieved by ion-exchange chromatography with Sp-Sephadex C-50 by our earlier method. The sugars remaining after removal of lactose by crystallization were fractionated by gel filtration chromatography(Bio-gel P-2) with distilled water as eluent. On the otherhand, the colostrum glycopeptide was treated with NaOH of NaB <H_4> for 36h at 50 C in the dark under <N_2> , followed by removal of low-molecular peptides and salts using Dowex 50W-X4 resin. The resulting oligosacchariditols were finally purified by gel filtration chromatography(Biogel P-2) with distilled water as eluent. The purified oligosaccharditols were fractionated by ion-exchange chromatography using the method of Farrar et al in a linear concentration gradient of 0.05-1.0M of pyridine-acetate buffer.

分娩后6H荷斯坦 - 弗里斯牛（Holstein-Friesian Cow）取结果。 Schneir和Rafelson的方法通过透析从透析中分离出乳糖的牛奶糖。通过我们较早的方法，用SP-Sephadex C-50通过离子交换色谱法实现了从透析物中完全去除肽。通过结晶去除乳糖后剩余的糖通过凝胶过滤色谱法（Bio-Gel P-2）用蒸馏水作为洗脱液来分级。另一方面，在<n_2>下，在黑暗中用NaH的NaOH在50 C下用NaOH处理初乳糖肽，然后使用dowex 50W-X4树脂去除低分子肽和盐。最终，用蒸馏水作为洗脱液，通过凝胶过滤色谱法（Biogel P-2）纯化所得的寡糖醇。使用Farrar等人的方法在线性浓度梯度为0.05-1.0m的吡啶 - 乙酸酯缓冲液中，通过离子交换色谱法分离纯化的寡核酸脂蛋白。

项目成果

Biochimica et Biophysica Acta. 801-. (1984)

生物化学与生物物理学学报。

Biochimica et Biophysica Acta. 801. (1984)

生物化学与生物物理学学报。