The influence of transcript localization on post-translational modification

转录本定位对翻译后修饰的影响

• 批准号: 23657137
• 负责人: YAMAMOTO Miki
• 金额: $ 2.5万
• 依托单位: Rikkyo University (2012)Keio University (2011)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-23657137/
• 关键词: 細胞構造; 機能; GPIアンカー修飾; 翻訳後修飾; GPI; mRNA

Post-translational modification of protein is important in promoting proper function of protein. In this study, we investigated whether post-translational modification of protein is regulated by mRNA localization or not. Dally-like protein (Dlp) in Drosophila has GPI-anchor which is a type of post-translational modification. And dlp mRNA shows restricted distribution near nucleus in wing disc cells. We changed the localization of dlp mRNA by replacing 3’-noncoding region of dlp with that of wingless which contains apical localization signal. The products translated from mislocalized mRNA did not show proper function and were accumulated in apical region of cells, suggesting that mRNA localization is involved in protein function and probably post-translationalmodification.

蛋白质的翻译后修饰对于促进蛋白质的正常功能非常重要，在本研究中，我们研究了果蝇中具有 GPI 锚的 mRNA 定位是否调节蛋白质的翻译后修饰。是一种翻译后修饰，并且 dlp mRNA 在翼盘细胞中显示出限制性分布。我们通过替换 dlp 的 3'-非编码区来改变 dlp mRNA 的定位。与含有顶端定位信号的wingless相比，由错误定位的mRNA翻译的产物没有表现出适当的功能，并且在细胞的顶端区域积累，这表明mRNA定位涉及蛋白质功能并且可能涉及翻译后修饰。

项目成果

Novel Roles of Glycosylation in Drosophila Innate Immunity

糖基化在果蝇先天免疫中的新作用

• 发表时间: 2012
• 作者: Takahashi A;Kimura T;Takabatake Y;Namba T;Kaimori J;Kitamura H*;Matsui I;Niimura F;Matsusaka T;Fujita N;Yoshimori T;Isaka Y*;Rakugi H.;山本(日野)美紀
• 通讯作者: 山本(日野)美紀

Identification of proteasome components required for apical localization of Chaoptin using functional genomics

使用功能基因组学鉴定 Chaoptin 顶端定位所需的蛋白酶体成分

• 发表时间: 2012
• 期刊: J. Neurogenet.
• 作者: Yano,H.;Yamamoto-Hino,M.;Awano,W.;Aoki- Kinoshita,K.F.;Tsuda-Sakurai,K.;Okano,H. and Goto,S.
• 通讯作者: Okano,H. and Goto,S.

Cisterna-specific Localization of Glycosylation-related Proteins to the Golgi Apparatus

糖基化相关蛋白对高尔基体的水池特异性定位

• DOI: 10.1247/csf.11037
• 发表时间: 2012
• 期刊: Cell Structure and Function
• 影响因子: 1.5
• 作者: Yamamoto-Hino;M.;Abe;M.;Shibano;T.;Setoguchi;Y.;Awano;W.;Ueda. R.;Okano;H.;Goto;S.
• 通讯作者: S.

Golgi complex-related glycoprotein 1 and its function in innate immunity

高尔基复合体相关糖蛋白1及其在先天免疫中的作用

• 发表时间: 2013
• 作者: Raju D;Hussey S;Ang M;Terebiznik MR;Sibony M;Galindo-Mata E;Gupta V;Blanke SR;Delgado A;Romero-Gallo J;Ramjeet MS;Mascarenhas H;Peek RM;Correa P;Streutker C;Hold G;Kunstmann E;Yoshimori T;Silverberg MS;Girardin SE;Philpott DJ;El Omar ;山本（日野）美紀
• 通讯作者: 山本（日野）美紀

ショウジョウバエゴルジ体に局在する Golgi complex-relatedglycoprotein1の同定と自然免疫における機能

果蝇高尔基体高尔基复合体相关糖蛋白1的鉴定及其在先天免疫中的功能

• 发表时间: 2013
• 作者: 山本(日野)美紀