Signal Transduction and Control of T-cell function by Prolactin

催乳素对 T 细胞功能的信号转导和控制

• 批准号: 05453176
• 负责人: NAKASHIMA Kunio
• 金额: $ 4.74万
• 依托单位: Mie University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05453176/
• 关键词: Rat; Nb2 lymphoma cell; G1 cyclin; Cyclin D2; Cyclin D3; Gene expression; Promoter; Prolactin; プレーTリンフォーマ; Nb2; 下細胞分化増殖; プロククチン; Nb2細胞; T抗原レセプターγ; サイクリンD2・D3; シグナル伝達経路; 遺伝子発現制御; G1; S移行; T細胞分化; T細胞抗原γ鎖

Prolactin has been demonstrated to induce the expression of the genes encoding cyclin D2 and D3 during promotion of the G1/S transition in rat Nb2 pre-T lymphoma cells. cDNA clones encoding these rat cyclins D2 and D3 were isolated and sequenced. The genes for cyclin E,cdk2, cdk5 and E2F-1 are also expressed synergistically at the same stage, suggesting the presence of a common cellular mechanism to stimulate these genes. It has also been found that, during the same G1/S transition phase, prolactin stimulates TCR-nu gene expression in 4 h and represses TCR-alpha and beta genes in the Nb2 cells. These results suggest that prolactin participates in the regulation of mammalian T-cell differentiation and proliferation.

催乳素已被证明是在促进大鼠NB2前-T淋巴瘤细胞中G1/S转变期间诱导编码细胞周期蛋白D2和D3的基因的表达。分离并测序编码这些大鼠细胞周期蛋白D2和D3的cDNA克隆。细胞周期E，CDK2，CDK5和E2F-1的基因也在同一阶段协同表达，这表明存在刺激这些基因的常见细胞机制。还发现，在相同的G1/S转变阶段，催乳素刺激4小时中的TCR-NU基因表达，并抑制NB2细胞中的TCR-α和β基因。这些结果表明，催乳素参与了哺乳动物T细胞分化和增殖的调节。

项目成果

Yoshitaka Hosokawa 他: "Synergistic gene expressions of cyclin E, cdk2, cdk5 and E2F during the prolactin-induced G1/S transitlon in rat Nb2 pre-T lymphom2 cells" Biochem. Mol. Biol. International. 37. 393-399 (1995)

Yoshitaka Hosokawa 等人：“大鼠 Nb2 前 T 淋巴瘤细胞中催乳素诱导的 G1/S 转变过程中细胞周期蛋白 E、cdk2、cdk5 和 E2F 的协同基因表达”Biochem International。 (1995)

Yoshitaka Hosokawa, Miao Yang, Satoshi Kaneko, Minoru Tanaka and Kunio Nakashima: "Prolactin induces switching of T-cell receptor gene expression from alpha to nu in rat Nb2 pre-T lymphoma cells." Biochem.Biophys.Res.Commun.(in press). (1996)

Yoshitaka Hosokawa、Miao Yang、Satoshi Kaneko、Minoru Tanaka 和 Kunio Nakashima：“催乳素诱导大鼠 Nb2 前 T 淋巴瘤细胞中 T 细胞受体基因表达从 alpha 转变为 nu。”

Kesami Sakaguchi, Minoru Tanaka, Takeshi Ohkubo, Katsumi Doh-ura, Takahiko Fujikawa, Shu Sudo and Kunio Nakashima: "Induction of brain prolactin receptor long form mRNA expression and maternal behavior in the pup-contacted male rats : Promotion by prolact

Kesami Sakaguchi、Minoru Tanaka、Takeshi Ohkubo、Katsumi Doh-ura、Takahiko Fujikawa、Shu Sudo 和 Kunio Nakashima：“在幼仔接触的雄性大鼠中诱导脑催乳素受体长型 mRNA 表达和母性行为：催乳素的促进

Takashi Fujikawa, Hideaki Soya, Hideo Yoshizato, Kesami Sakaguchi, Katsumi Doh-ura, Minoru Tanaka and Kunio Nakashima: "Restraint stress enhaces the gene expression of prolactin receptor long form at the choroid plexus." Endocrinology. 136(12). 5608-5613

Takashi Fujikawa、Hideaki Soya、Hideo Yoshizato、Kesami Sakaguchi、Katsumi Doh-ura、Minoru Tanaka 和 Kunio Nakashima：“束缚压力增强了脉络丛催乳素受体长型的基因表达。”

Yoshitaka Hosokawa,他: "Prolactin induces switching of T-cell receptor gene expression from α to γ in rat Nb2 pre-T lymphoma cells" Biochem.Biophys.Res.Commun.(印刷中). (1996)

Yoshitaka Hosokawa 等人：“催乳素诱导大鼠 Nb2 前 T 淋巴瘤细胞中 T 细胞受体基因表达从 α 转变为 γ”Biochem.Biophys.Res.Commun.（出版中）。