Studies on the Gene Expression and Physiological Significance of novel Prolactin-Like Proteins in Bovine Placenta

牛胎盘中新型催乳素样蛋白的基因表达及生理意义研究

• 批准号: 01570128
• 负责人: NAKASHIMA Kunio
• 金额: $ 1.41万
• 依托单位: MIE University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1990
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-01570128/
• 关键词: Bovine placenta; Prolactin-like proteins; cDNA cloning; bPLP-I; bPLP-II; bPLP-III; bPLP-IV; GH; PRL gene family; ウシプロラクチン様ホルモン; PRL; GH遺伝子ファミリ-; C末端ジスルフィド結合; 成長ホルモン; プロラクチン; 胎盤性ラクトゲン; 遺伝子ファミリ-; bPLP

Four novel prolactin-like hormone cDNA clones have been isolated from a bovine term placenta cDNA library. We designated these clones as bovine prolactin-like protein (bPLP)-I, II. III and IV. bPLP-I, II, III, IV showed 50, 48, 43 and 48% homology of amino acid sequence with that of bovine prolactin, respectively. With bovine growth hormone, however, they showed only 23, 23, 22 and 22% homology, respectively. bPLP-I, on the other hand, was identical except for the N-terminal amino acid residue with bovine placental lactogen. bPLP-I has Val as N-terminal. when bovine placental lactogen has Ala for that position. Predicted mature forms of bPLP-I, II, III and IV are composed of 201, 202, 203 and 201 amino acid residues, respectively. Gene expressions of these four novel prolactin-like hormones the in bovine placenta have been analyzed by Northern blot hybridization and turned out to be stage-specific being maximal in the full-term-placenta. Thus, four novel bovine placental hormones bPLP-I, II, III and IV, resembled prolactin more closely than a growth hormone, and were distinct from placental lactogen. And these new hormones were considered to play important roles in metabolism and growth of the bovine fetus.

已经从牛胎盘cDNA文库中分离出四个新型催乳素样激素cDNA克隆。我们将这些克隆指定为牛催乳素样蛋白（BPLP）-I，II。 III和IV。 BPLP-I，II，III，IV分别显示了50、48、43和48％与牛催乳素的氨基酸序列的同源性。然而，由于牛生长激素，它们仅显示23％，23％，22％和22％的同源性。另一方面，BPLP-I除了牛胎盘泌乳基因的N末端氨基酸残基外是相同的。 BPLP-I具有N末端。当牛胎盘乳酸具有该位置的ALA时。 BPLP-I，II，III和IV的成熟形式分别由201、202、203和201氨基酸残基组成。这四种新型催乳素样激素的基因表达已经通过北印迹杂交分析了牛胎盘中的基因表达，并被证明是在完整植物的最大阶段。因此，与生长激素相比，四种新型的牛胎盘激素BPLP-I BPLP-I，II，III和IV比催乳素更接近催乳素，并且与胎盘乳乳元素不同。这些新激素被认为在牛胎儿的新陈代谢和生长中起着重要作用。

项目成果

M.Yamakawa,M.Tanaka,M.Koyama,Y.Kagesato,M.Watahiki,M.Yamamoto and K.Nakashima: "Expression of new members of the prolactinーgrowth hormone gene family in bovine placenta" J.Biol.Chem.265. 8915-8920 (1990)

M. Yamakawa、M. Tanaka、M. Koyama、Y. Kagesato、M. Watahiki、M. Yamamoto 和 K. Nakashima：“催乳素生长激素基因家族新成员在牛胎盘中的表达” J.Biol.Chem .265.8915-8920（1990）

M. Watahiki M. Tanaka N. Masuda, K. Sugisaki, M. Yamamoto, M. Yamakawa, J. Nagai and K. Nakashima: ""Primary structure of chicken pituitary prolactin deduced from the cDNA sequence : Conserved and specific amino acid residues in the domains of the prolact

M. Watahiki M. Tanaka N. Masuda、K. Sugisaki、M. Yamamoto、M. Yamakawa、J. Nagai 和 K. Nakashima：“”从 cDNA 序列推导出的鸡垂体催乳素的一级结构：保守且特异的氨基酸残基

中島邦夫: "新生化学入門" 南山堂株式会社, 280 (1990)

中岛邦男：《新生物化学导论》 Nanzando Co., Ltd.，280（1990）

M.Yamakawa,M.Tanaka,M.Koyama,Y.Kagesato,M.Watahiki,M.Yamamoto and K.Nakashima: "Expression of new members of the prolactin growth hormone gene family in bovine placenta" J.Biol.Chem.265. 8915-8920 (1990)

M.Yamakawa、M.Tanaka、M.Koyama、Y.Kagesato、M.Watahiki、M.Yamamoto 和 K.Nakashima：“催乳素生长激素基因家族新成员在牛胎盘中的表达”J.Biol.Chem。

Masanori Watahiki: "Primary structure of chicken pituitary prolactin deduced from the cDNA sequence:conserved and specific amino acid residues in the domains of the prolactins." The Journal of Biological Chemistry. 264. 5535-5539 (1989)

Masanori Watahiki：“从 cDNA 序列推导出鸡垂体催乳素的一级结构：催乳素结构域中保守且特定的氨基酸残基。”