Influence of Saponins on Lipid Bilayers

皂苷对脂质双层的影响

• 批准号: 315438106
• 负责人: Professor Dr. Thomas Hellweg
• 金额: --
• 依托单位: Arbeitsgruppe Physikalische und Biophysikalische Chemie (PC III)
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2016
• 资助国家: 德国
• 起止时间: 2015-12-31 至 2018-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/315438106?language=en
• 关键词: Influence; Saponins; Lipid; Bilayers

Saponins have a multitude of different effects in living systems. In most cases these effects can be related to the interaction of saponins with the cell membranes. For this reason saponins are studied intensely since many decades. However, despite the pharmacological importance and despite of the numerous studies on saponins information about the local interaction between lipids, cholestrol and saponins is scarce. The present project we will study model membranes (vesicle) made of different lipids and saponins in presence of different amounts of cholesterol by means of neutron spin-echo spectroscopy. The quasielastic experiments will be complemented by small angle and wide angle scattering aiming at establishing relations between local structure and bending elasticity of the bilayers. In addition photon correlation spectroscopy and flickering spectroscopy will be used to scrutinize the membrane motion on larger length and time scales. We are aiming at establishing a relationschip between saponin induced changes in membrane properties to the known biological effects of saponins.

皂苷在生活系统中具有多种不同的影响。在大多数情况下，这些作用可能与皂苷与细胞膜的相互作用有关。因此，自数十年以来，对皂苷进行了深入的研究。然而，尽管有药理意义，尽管有许多有关皂苷的研究，但有关脂蛋白，胆脂质和皂苷之间局部相互作用的信息稀缺。本项目，我们将通过中子自旋回波光谱法研究在不同量的胆固醇存在下，由不同含量的胆固醇制成的膜（囊泡）模拟膜（囊泡）。准层实验将以旨在建立局部结构和双层弯曲弹性之间关系的小角度和广角散射来补充。另外，光子相关光谱和闪烁光谱法将用于仔细检查较大的长度和时间尺度上的膜运动。我们旨在在皂苷诱导的膜特性变化与皂苷的已知生物学作用之间建立关系。