Treatment with growth factor prosaposin in a mammalian model of spina bifida

生长因子前塞塞蛋白治疗哺乳动物脊柱裂模型

基本信息

批准号：
21K09324
负责人：
カーンシャキル
金额：
$ 2.66万
依托单位：
Oita University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2021
资助国家：
日本
起止时间：
2021-04-01 至 2024-03-31
项目状态：
已结题

项目摘要

Spina bifida aperta (SBA), a developmental disorder of the spinal cord, causes high pediatric mortality and is the leading cause of lifelong neurological complications including paralysis, orthopedic abnormalities, problems with bladder and bowel control, and painful complications. Current therapeutics for SBA have various limitations, including incomplete preservation of spinal tissue and the inability to rescue sensorimotor functions. We found that intra-amniotic delivery of prosaposin-derived 18 mer-peptide (PS18), a potential neurotrophic factor, to the chick embryo with SBA enhances the regeneration process and protects spinal tissue in developing spinal cord. Furthermore, treatment with PS18 dramatically improved the walking and bowel movement ability and reduce and painful complications of hatched chicks in postnatal life. The results are published as first author in a highly reputed scientific journal (Khan et al., iScience 2023; IF: 6.107).In order to aim for clinical application of PS18, verification using not only the chicken model but also the mammalian SBA model is necessary. In this regard, we are trying to develop a mice model of SBA by inducing Zika virus which causes neural tube malformations and other birth defects. Zika virus solution (1x10^4 PFU in 200μL PBS) was administered subcutaneously to STAT1 KO mice on the 6th day of pregnancy to induce SBA and/or other defects in the fetus. Once a mice model with SBA or other neurological birth defects is developed, we will evaluate the therapeutic efficacy of PS18 with the aim of writing papers.

脊柱裂 (SBA) 是一种脊髓发育障碍，会导致儿童高死亡率，并且是导致终生神经系统并发症的主要原因，包括瘫痪、骨科异常、膀胱和肠道控制问题以及疼痛并发症。我们发现，羊膜内递送前列腺素衍生的 18 聚体肽 (PS18)，这是一种潜在的神经营养物质。因子，用 SBA 增强鸡胚的再生过程并保护发育中的脊髓组织。此外，用 PS18 治疗可显着改善孵化鸡的行走和排便能力，并减少出生后生活中的痛苦并发症。作为知名科学期刊的第一作者 (Khan et al., iScience 2023; IF: 6.107)。为了实现 PS18 的临床应用，不仅使用鸡模型，还使用在这方面，我们正在尝试通过诱导寨卡病毒来开发小鼠SBA模型，该模型会导致神经管畸形和其他出生缺陷，并给予寨卡病毒溶液（1x10^4 PFU于200μL PBS中）。在怀孕第 6 天皮下注射 STAT1 KO 小鼠以诱导胎儿 SBA 和/或其他缺陷一旦开发出具有 SBA 或其他神经出生缺陷的小鼠模型，我们将进行研究。评估PS18的治疗效果，以撰写论文为目的。

项目成果

期刊论文数量（35）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

The Safety and Efficacy of a Therapeutic Vaccine for Chronic Hepatitis B: A Follow-Up Study of Phase III Clinical Trial

慢性乙型肝炎治疗疫苗的安全性和有效性：III期临床试验的后续研究

DOI：
10.3390/vaccines10010045
发表时间：
2021
期刊：
影响因子：
7.8
作者：
Akbar SMF; Al Mahtab M; Aguilar JC; Yoshida O; Khan S; Penton E; Gerardo GN; Hiasa Y
通讯作者：
Hiasa Y

Prosaposin in the rat oviductal epithelial cells

大鼠输卵管上皮细胞中的前列腺素

DOI：
10.1007/s00441-020-03339-x
发表时间：
2020
期刊：
影响因子：
3.6
作者：
Shimokawa Tetsuya;Nabeka Hiroaki;Khan Sakirul Islam;Yamamiya Kimiko;Doihara Takuya;Kobayashi Naoto;Wakisaka Hiroyuki;Matsuda Seiji
通讯作者：
Matsuda Seiji

Surge of Dengue fever during COVID-19 pandemic in Bangladesh: Similar symptoms and difficulties in diagnosis with devastating outcome

孟加拉国 COVID-19 大流行期间登革热激增：类似症状和诊断困难，造成灾难性后果