Excellence in Research: Evaluating Environmental Stressor Effects on Soil Bacterial Traits

卓越研究：评估环境压力因素对土壤细菌性状的影响

• 批准号: 2302609
• 负责人: Danielle Graham
• 金额: $ 76.92万
• 依托单位: Fayetteville State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-06-01 至 2026-05-31
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2302609&HistoricalAwards=false
• 关键词: Excellence; Research; Evaluating; Environmental; Stressor

The goal of this Excellence in Research (EiR) project is to conduct interdisciplinary, collaborative research to investigate how prior environmental nutrient conditions influence bacterial inter- and intraspecific interactions. Specifically, the research will examine how changes in nutrients alter bacterial traits such as the physical features of forming spores and modifying cell walls and genetic features that are responsible for changes to physical features, antibiotic resistance, and soil nutrient access in the context of microbe-microbe and plant-microbe relationships. Results from this work are of broad national interests in agricultural, natural resources management and conservation sectors. This work benefits society through research opportunities at the undergraduate and graduate levels and through shared educational content for grades 6-8 at rural public schools. This research will be integrated with the education of undergraduates at Fayetteville State University (FSU), a Historically Black University focusing on the education of African American students, with high female, and military or military-affiliated student populations. This project will support teaching-research integration capacity through the design, implementation, and assessment of a course-based undergraduate research experience (CURE) offered at FSU, which will be linked to ongoing CUREs and undergraduate/graduate student-led projects at East Carolina University (ECU). The integration of microbial ecology and biodiversity research will improve the STEM education quality of historically marginalized populations and enhance diversity and training of the STEM workforce. This research will test the hypothesis that long-term nutrient enrichment will result in more competitive (and less mutualistic) bacterial populations (e.g., increased antimicrobial resistance, increased growth rates) compared to the unfertilized counterparts by focusing on three objectives: 1) examining culturable wetland soil bacteria by trait-based phenotypic analyses to understand how ongoing nutrient enrichment influences bacterial traits that are important for resource acquisition, 2) identifying genotypic differences between bacterial isolates from nutrient-rich vs. nutrient-limited environments using comparative genomic approaches, and 3) examining how previous nutrient conditions influence microbe-microbe and plant-microbe interactions by adding bacterial inocula (from fertilized or unfertilized soils) to a wetland grass exposed to a fertilization gradient. The Fayetteville State University and East Carolina University teams will collaboratively conduct comparative genomics and functional lab-based assays. Evaluating bacterial traits in an ecological context will expand our understanding of how resource limitation influences soil microbial trait diversity with consequences for promoting beneficial plant-microbe interactions in agriculture and restoration. Collectively, this research will provide participants the opportunity to generate new knowledge, establish and refine critical thinking skills, and enhance the overall competitiveness of students entering graduate and/or professional school as well as the workforce.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

这项卓越研究（EIR）项目的目的是进行跨学科的协作研究，以研究先前的环境营养状况如何影响细菌间和种内相互作用。具体而言，该研究将研究养分的变化如何改变细菌性状，例如形成孢子的物理特征，修饰细胞壁和遗传特征，这些特征造成了物理特征，抗生素耐药性以及在微生物 - 菌群和植物菌方关系的情况下变化的变化。这项工作的结果是农业，自然资源管理和保护部门的广泛国家利益。这项工作通过在本科和研究生级别的研究机会以及通过在农村公立学校的6 - 8年级共享教育内容来使社会受益。这项研究将与费耶特维尔州立大学（FSU）的本科生的教育融合，这是一所历史悠久的黑人大学，专注于非裔美国学生的教育，女性高，是女性，军事或军事相关的学生人数。该项目将通过设计，实施和评估FSU提供的基于课程的本科研究经验（CURE）的设计，实施和评估，该项目将与持续的治疗方法以及East Carolina University（ECU）的本科/研究生领导的项目有关。微生物生态学和生物多样性研究的整合将提高历史边缘化人群的STEM教育质量，并增强STEM劳动力的多样性和培训。这项研究将检验以下假设：与未经施用的物体相比，与未经许可的对应物相比，与未经施用的对应物相比，将基于特质的湿润的湿润分析构成了越来越多的杂种，将导致更具竞争力（且较少相互的）细菌种群（例如，抗菌耐药性增加，增长抗菌耐药性增加，生长速率提高），从而构成了跨性别的细菌，从对于资源获取重要的重要，2）使用比较基因组方法从富含营养的富含营养和养分限制的环境中鉴定细菌分离株之间的基因型差异，以及3）研究以前的养分状况如何影响微生物微生物和植物 - 微生物，通过添加细菌接种物（从污染的土壤中添加肥料），从而添加细菌的相互作用，从而获得湿的土壤，以降级土壤，从而获得湿润的土壤）。费耶特维尔州立大学和东卡罗来纳大学团队将协作进行比较基因组学和功能实验室的测定法。在生态环境中评估细菌性状将扩大我们对资源限制如何影响土壤微生物特征多样性的理解，并在促进农业和恢复中促进有益的植物微生物相互作用的后果。总的来说，这项研究将为参与者提供建立新知识，建立和完善批判性思维能力的机会，并增强进入研究生和/或专业学校以及劳动力的学生的整体竞争力。该奖项反映了NSF的法定任务，并认为通过基金会的知识分子和更广泛的影响，可以通过评估来进行支持，以审查Criteria。