Biogenesis of thylakoid membranes in cyanobacteria

蓝藻类囊体膜的生物发生

• 批准号: 254834189
• 负责人: Professor Dr. Jörg Nickelsen, Ph.D.
• 金额: --
• 依托单位: Arbeitsgruppe Molekulare Pflanzenwissenschaften
• 依托单位国家: 德国
• 项目类别: Research Units
• 财政年份: 2014
• 资助国家: 德国
• 起止时间: 2013-12-31 至 2021-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/254834189?language=en
• 关键词: Biogenesis; thylakoid; membranes; cyanobacteria

Assembly of cyanobacterial photosystem II (PSII) proceeds in a highly-ordered, step-wise manner and is initiated at specialized biogenic membranes close to the plasma membrane, named biogenesis centers. These regions are characterized by the accumulation of the manganese delivery factor PratA and are shaped by the membrane curving protein CurT. In this project, it is aimed at further elucidating the assembly factor network involved in cyanobacterial PSII biogenesis. By using PratA and CurT as well as their identified physical and/or functional interaction partners as molecular entry points, genetic and biochemical techniques will be applied to dissect the spatiotemporal organization of the assembly process. This additionally includes the dismantling of the precise structure and dynamics of biogenic membrane regions in Synechocystis 6803 by following cryo-electron tomography- and livecell imaging-based approaches.

蓝藻光系统 II (PSII) 的组装以高度有序、逐步的方式进行，并在靠近质膜的特殊生物膜（称为生物发生中心）处启动。这些区域的特征是锰传递因子 PratA 的积累，并由膜弯曲蛋白 CurT 塑造。本项目旨在进一步阐明参与蓝藻 PSII 生物发生的组装因子网络。通过使用 PratA 和 CurT 以及它们确定的物理和/或功能相互作用伙伴作为分子入口点，将应用遗传和生化技术来剖析组装过程的时空组织。这还包括通过以下基于冷冻电子断层扫描和活细胞成像的方法来拆除集胞藻 6803 中生物膜区域的精确结构和动力学。