Biogenesis and molecular architecture of native thylakoid membranes investigated by in situ cryo-electron tomography

通过原位冷冻电子断层扫描研究天然类囊体膜的生物发生和分子结构

• 批准号: 387117610
• 负责人: Professor Dr. Benjamin Engel, Ph.D.
• 金额: --
• 依托单位: Biozentrum
• 依托单位国家: 德国
• 项目类别: Research Units
• 财政年份: 2017
• 资助国家: 德国
• 起止时间: 2016-12-31 至 2022-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/387117610?language=en
• 关键词: Biogenesis; molecular; architecture; native; thylakoid

The light-dependent reactions of photosynthesis are orchestrated by the intricate architecture of the thylakoid membranes. However, the mechanisms that establish and maintain thylakoid architecture are poorly understood. In this project, we will explore the molecular landscapes of eukaryotic and prokaryotic photosynthetic organisms (Chlamydomonas reinhardtii and Synechcocystis sp. PCC 6803) by in situ cryo-electron tomography. Vitreous frozen cells are thinned with a focused ion beam and then imaged by cryo-electron tomography, revealing native cellular environments with molecular detail. Using these unprecedented views into the cellular interior, we will investigate: 1) the molecular organization of photosynthetic complexes in relation to thylakoid architecture and regions of thylakoid biogenesis, 2) the structure of thylakoid-bound ribosomes and their distribution relative to biogenetic regions, and 3) the structure oligomeric VIPP1 and the role of this protein complex in facilitating thylakoid biogenesis. These investigations will be carried out in close collaboration with other groups in FOR2092.

光合作用的光依赖性反应是由类囊体膜的复杂结构精心安排的。然而，人们对建立和维持类囊体结构的机制知之甚少。在这个项目中，我们将通过原位冷冻电子断层扫描探索真核和原核光合生物（莱茵衣藻和聚胞藻属 PCC 6803）的分子景观。使用聚焦离子束对玻璃体冷冻细胞进行减薄，然后通过冷冻电子断层扫描进行成像，以分子细节揭示天然细胞环境。利用这些前所未有的细胞内部视角，我们将研究：1）与类囊体结构和类囊体生物发生区域相关的光合复合物的分子组织，2）类囊体结合核糖体的结构及其相对于生物发生区域的分布，以及3）寡聚VIPP1的结构以及该蛋白复合物在促进类囊体生物发生中的作用。这些调查将在 FOR2092 中与其他小组密切合作进行。