Coordination Funds

协调基金

• 批准号: 387077896
• 负责人: Professor Dr. Jörg Nickelsen, Ph.D.
• 金额: --
• 依托单位: Arbeitsgruppe Molekulare Pflanzenwissenschaften
• 依托单位国家: 德国
• 项目类别: Research Units
• 财政年份: 2017
• 资助国家: 德国
• 起止时间: 2016-12-31 至 2021-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/387077896?language=en
• 关键词: Coordination; Funds

Photosynthetic electron transport is mediated by multi-subunit pigment-protein complexes which are situated in a specialized membrane system, named thylakoids. Despite a detailed knowledge on the structure and function of these complexes, little is known on their assembly during thylakoid membrane biogenesis. The emerging picture of this process depicts a highly-ordered scaffold of assembly factors that integrates the incorporation of proteins and organic as well as inorganic co-factors in a step-wise manner. Recent work has also indicated that the production line for photosynthetic complexes is initiated at biogenic membrane sub-compartments from where it proceeds via discrete and conserved assembly intermediates to generate a functional energy converting apparatus. The FOR2092 Research Unit has set out to disentangle the molecular principles of the spatiotemporal organization of thylakoid membrane biogenesis by applying a multidisciplinary, systematic approach combining unique expertise in molecular genetics, biochemistry, biophysics and ultrastructural analyses. The concept includes the comparative investigation of a set of suitable model organisms that enables one to follow the complete evolutionary path for the development of thylakoid complexity from primordial cyanobacteria to chloroplasts of vascular plants. By focusing on distinct assembly factors and the spatiotemporal organization of their working mode, the joined forces of FOR2092 members have recently discovered a variety of new molecular aspects of thylakoid biogenesis. The findings include details of photosystem II and photosystem I assembly, membrane insertion of thylakoid proteins, organic and inorganic co-factor incorporation and formation of biogenic membrane structures. In addition, both genetic and biochemical approaches have identified several new components of the intricate assembly factor network for thylakoid biogenesis. In sum, this obtained body of evidence now forms the solid basis for a proceeding comprehensive study of the biogenesis process which will be complemented by comparative analysis of the pigment-free ATPase complex as well as state-of-the-art studies on thylakoid ultrastructure in situ. The consortium envisions that by answering the questions how, where and when the different assembly processes take place and are integrated during thylakoid maturation, the development of knowledge-based strategies for the targeted modification of the biogenesis process in a broad set of photosynthetic model organisms will become feasible in the future.

光合电子传输是由位于专门的膜系统（称为类囊体）中的多生育色素 - 蛋白质复合物介导的。尽管对这些复合物的结构和功能有详细的了解，但在类囊体膜生物发生过程中，它们在组装上几乎没有知识。此过程的新兴图片描绘了一个高度排序的组装因子支架，这些脚手架以逐步的方式整合了蛋白质和有机物以及无机co剂的掺入。最近的工作还表明，光合络合物的生产线是在生物膜子室中启动的，从那里它通过离散和保守的组装中间体进行，以生成功能性能量转化设备。 FOR2092研究单元已旨在通过应用多学科的系统方法，结合了分子遗传学，生物化学，生物物理学和超结构分析的独特专业知识，通过应用多学科的系统方法来解开类囊体膜生物发生的分子原理。该概念包括对一组合适的模型生物的比较研究，使人们能够遵循从原始蓝细菌到血管植物的叶绿体发展的囊体复杂性的完整进化途径。通过关注不同的组装因子和其工作模式的时空组织，For2092成员的联合力量最近发现了类囊体生物发生的各种新分子方面。这些发现包括光系统II和光系统I组装的细节，类囊体蛋白的膜插入，有机和无机的共同因素结合和生物膜结构的形成。此外，遗传和生化方法都确定了用于类囊体生物发生的复杂装配因子网络的几个新组成部分。总而言之，这一获得的证据现在构成了对生物发生过程进行综合研究的稳定基础，该研究将通过对无颜料ATPase复合物的比较分析以及对囊类超微结构原位的最新研究进行补充。该财团设想，通过回答问题，在类囊体成熟过程中进行不同的组装过程的方式，何时何地和何时进行，即在一系列广泛的光合作用模型生物体中的有针对性修饰基于知识的策略的发展将在未来变得可行。