Coordination Funds

协调基金

• 批准号: 387077896
• 负责人: Professor Dr. Jörg Nickelsen, Ph.D.
• 金额: --
• 依托单位: Arbeitsgruppe Molekulare Pflanzenwissenschaften
• 依托单位国家: 德国
• 项目类别: Research Units
• 财政年份: 2017
• 资助国家: 德国
• 起止时间: 2016-12-31 至 2021-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/387077896?language=en
• 关键词: Coordination; Funds

Photosynthetic electron transport is mediated by multi-subunit pigment-protein complexes which are situated in a specialized membrane system, named thylakoids. Despite a detailed knowledge on the structure and function of these complexes, little is known on their assembly during thylakoid membrane biogenesis. The emerging picture of this process depicts a highly-ordered scaffold of assembly factors that integrates the incorporation of proteins and organic as well as inorganic co-factors in a step-wise manner. Recent work has also indicated that the production line for photosynthetic complexes is initiated at biogenic membrane sub-compartments from where it proceeds via discrete and conserved assembly intermediates to generate a functional energy converting apparatus. The FOR2092 Research Unit has set out to disentangle the molecular principles of the spatiotemporal organization of thylakoid membrane biogenesis by applying a multidisciplinary, systematic approach combining unique expertise in molecular genetics, biochemistry, biophysics and ultrastructural analyses. The concept includes the comparative investigation of a set of suitable model organisms that enables one to follow the complete evolutionary path for the development of thylakoid complexity from primordial cyanobacteria to chloroplasts of vascular plants. By focusing on distinct assembly factors and the spatiotemporal organization of their working mode, the joined forces of FOR2092 members have recently discovered a variety of new molecular aspects of thylakoid biogenesis. The findings include details of photosystem II and photosystem I assembly, membrane insertion of thylakoid proteins, organic and inorganic co-factor incorporation and formation of biogenic membrane structures. In addition, both genetic and biochemical approaches have identified several new components of the intricate assembly factor network for thylakoid biogenesis. In sum, this obtained body of evidence now forms the solid basis for a proceeding comprehensive study of the biogenesis process which will be complemented by comparative analysis of the pigment-free ATPase complex as well as state-of-the-art studies on thylakoid ultrastructure in situ. The consortium envisions that by answering the questions how, where and when the different assembly processes take place and are integrated during thylakoid maturation, the development of knowledge-based strategies for the targeted modification of the biogenesis process in a broad set of photosynthetic model organisms will become feasible in the future.

光合电子传递是由位于称为类囊体的特殊膜系统中的多亚基色素-蛋白质复合物介导的。尽管对这些复合物的结构和功能有详细的了解，但对其在类囊体膜生物发生过程中的组装知之甚少。这一过程的新图景描绘了一个高度有序的组装因子支架，它以逐步的方式整合了蛋白质和有机以及无机辅助因子的掺入。最近的工作还表明，光合复合物的生产线是在生物膜子室中启动的，从那里它通过离散和保守的组装中间体进行生产，以产生功能性能量转换装置。 FOR2092 研究小组已着手通过应用多学科、系统的方法，结合分子遗传学、生物化学、生物物理学和超微结构分析方面的独特专业知识，解开类囊体膜生物发生的时空组织的分子原理。该概念包括对一组合适的模式生物进行比较研究，使人们能够遵循从原始蓝细菌到维管植物叶绿体的类囊体复杂性发展的完整进化路径。通过关注不同的组装因子及其工作模式的时空组织，FOR2092 成员的联合力量最近发现了类囊体生物发生的各种新的分子方面。研究结果包括光系统 II 和光系统 I 组装、类囊体蛋白的膜插入、有机和无机辅因子掺入以及生物膜结构形成的详细信息。此外，遗传和生化方法都已鉴定出类囊体生物发生的复杂组装因子网络的几个新组成部分。总之，所获得的证据现在构成了对生物发生过程进行全面研究的坚实基础，并将通过无色素 ATP 酶复合物的比较分析以及类囊体超微结构的最先进研究来补充原地。该联盟设想，通过回答不同组装过程在类囊体成熟过程中如何、何地、何时发生和整合的问题，开发基于知识的策略，以有针对性地修改广泛的光合作用模型生物中的生物发生过程。未来变得可行。