Collaborative Research: RAPID: Microbiologic sampling of continental subsurface fluids from within the Cornell University Borehole Observatory (CUBO)

合作研究：RAPID：康奈尔大学钻孔观测站 (CUBO) 内大陆地下流体的微生物采样

• 批准号: 2231124
• 负责人: Stephanie Carr
• 金额: $ 1.52万
• 依托单位: Hartwick College
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-07-01 至 2024-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2231124&HistoricalAwards=false
• 关键词: Collaborative; Research; RAPID; Microbiologic; sampling

This project will collect samples of hot salty groundwater from roughly 1.5 to nearly 2 miles underground to study what microorganisms may live at these depths. The unusual opportunity to collect these samples comes from a deep well that is being drilled on Cornell University’s campus in upstate New York during Summer 2022. This deep environment is considered to be an extreme environment because of the warm temperature (150-200˚F), high salt concentration, lack of sunlight (energy), and lack of nutrients. Microbial cells will be counted from the collected samples to provide a better understanding of how much live lives underground. DNA will be analyzed to identify which types of microorganisms are living underground and to understand how those microorganisms survive in such an extreme environment. Ad¬vancing our knowledge of the diversity of microorganisms underground and how they survive can potentially provide insights into the origins of life on Earth and elsewhere in the universe. Additionally, this study will collect groundwater for chemical analyses that will better describe this extreme environment and aid efforts to grow the collected organisms in the laboratory. Collectively, cell counts, DNA, chemistry, and growth experiments will improve our understanding of life in the subsurface and life’s impacts to the chemistry of our planet. As part of this project, deep subsurface briny fluid samples will be collected upon completion of the Cornell University Borehole Observatory (CUBO), a DOE-funded 3-km deep geothermal exploratory well being drilled on Cornell’s upstate New York campus during summer 2022. Key objectives of the project are to: (1) collect and preserve water for geochemical analyses during a planned pump test, (2) quantify biomass concentrations using microscopy, (3) filter and preserve 100s of liters of fluid for genomic analyses, and (4) establish enrichment cultures. Sampling will target the Precambrian metamorphic / crystalline basement and overlying Paleozoic sedimentary sequences between 2.3 – 3.0 km depth where temperatures are expected to be ~ 70-95˚C. The large volume fluid sampling for geochemical and microbiologic characterization proposed here is not a part of the DOE-supported scope.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

该项目将从地下大约1.5到2英里收集热咸地下水的样本，以研究在这些深处可能居住的微生物。收集这些样品的不寻常机会来自2022年夏季纽约康奈尔大学校园的深层井。由于温度温暖（150-200˚F），高盐浓度，缺乏阳光（能量）和缺乏营养素，因此这种深度环境被认为是极端环境。微生物细胞将从收集的样品中计数，以更好地了解地下的生命。将对DNA进行分析，以确定哪些类型的微生物生活在地下，并了解这些微生物如何在如此极端的环境中生存。融入我们对地下微生物多样性以及它们如何生存的知识可以潜在地提供对地球和宇宙其他地方生命的起源的见解。此外，这项研究将收集地下水进行化学分析，以更好地描述这种极端的环境，并有助于在实验室中种植收集的生物。总体而言，细胞计数，DNA，化学和生长实验将改善我们对地下和生活对我们星球化学影响的影响的理解。 As part of this project, deep subsurface briny fluid samples will be collected upon completion of the Cornell University Borehole Observatory (CUBO), a DOE-funded 3-km deep geothermal exploration well being drilled on Cornell’s upstate New York campus during summer 2022. Key objects of the project are to: (1) collect and preserve water for geochemical analyses during a planned pump test, (2) quantify biomass使用显微镜的浓度，（3）过滤并保留100升液体进行基因组分析，（4）建立富集培养物。采样将针对前寒武纪变质 /晶体地下室，并在2.3 - 3.0 km的深度之间上覆盖古生代沉积序列，预计温度约为70-95°C。此处提出的用于地球化学和微生物特征的大容量流体采样并不是DOE支持的范围的一部分。该奖项反映了NSF的法定任务，并且认为值得通过基金会的知识分子优点和更广泛的影响审查标准来评估的支持。