Collaborative Research: RAPID: Microbiologic sampling of continental subsurface fluids from within the Cornell University Borehole Observatory (CUBO)

合作研究：RAPID：康奈尔大学钻孔观测站 (CUBO) 内大陆地下流体的微生物采样

• 批准号: 2231124
• 负责人: Stephanie Carr
• 金额: $ 1.52万
• 依托单位: Hartwick College
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-07-01 至 2024-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2231124&HistoricalAwards=false
• 关键词: Collaborative; Research; RAPID; Microbiologic; sampling

This project will collect samples of hot salty groundwater from roughly 1.5 to nearly 2 miles underground to study what microorganisms may live at these depths. The unusual opportunity to collect these samples comes from a deep well that is being drilled on Cornell University’s campus in upstate New York during Summer 2022. This deep environment is considered to be an extreme environment because of the warm temperature (150-200˚F), high salt concentration, lack of sunlight (energy), and lack of nutrients. Microbial cells will be counted from the collected samples to provide a better understanding of how much live lives underground. DNA will be analyzed to identify which types of microorganisms are living underground and to understand how those microorganisms survive in such an extreme environment. Ad¬vancing our knowledge of the diversity of microorganisms underground and how they survive can potentially provide insights into the origins of life on Earth and elsewhere in the universe. Additionally, this study will collect groundwater for chemical analyses that will better describe this extreme environment and aid efforts to grow the collected organisms in the laboratory. Collectively, cell counts, DNA, chemistry, and growth experiments will improve our understanding of life in the subsurface and life’s impacts to the chemistry of our planet. As part of this project, deep subsurface briny fluid samples will be collected upon completion of the Cornell University Borehole Observatory (CUBO), a DOE-funded 3-km deep geothermal exploratory well being drilled on Cornell’s upstate New York campus during summer 2022. Key objectives of the project are to: (1) collect and preserve water for geochemical analyses during a planned pump test, (2) quantify biomass concentrations using microscopy, (3) filter and preserve 100s of liters of fluid for genomic analyses, and (4) establish enrichment cultures. Sampling will target the Precambrian metamorphic / crystalline basement and overlying Paleozoic sedimentary sequences between 2.3 – 3.0 km depth where temperatures are expected to be ~ 70-95˚C. The large volume fluid sampling for geochemical and microbiologic characterization proposed here is not a part of the DOE-supported scope.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

该项目将将咸味咸的样品1.5到地下近2英里，以研究哪些微生物可能居住在这件事的THES THES THES THES THES THES THES可能会居住在Thes E的深处。在2022年的夏季，缺乏阳光（能量）和缺乏营养素微生物以及YES生存如何可以为地球和其他地方的生命的起源提供。援助在您的地球上集体影响您的化学。该项目的时间为：（1）在计划的泵测试期间收集并保留水以进行地球化学分析，（2）使用显微镜量化生物量浓度，过滤和保留100液的液体进行基因组分析（4）建立富集培养物前寒武纪的变质 /结晶地下室和上覆盖的古代沉积序列预计将是〜70-95°C，此处支撑的微生物表征不是DOE支持的scport。 SF'SFFLY的使命，并通过评估使用基金会的知识分子和更广泛的影响审查标准，通过评估获得了支持。