I-Corps: Massively Parallel High-Resolution Optical Electrophysiology

I-Corps：大规模并行高分辨率光学电生理学

• 批准号: 2225739
• 负责人: Ahmet Yanik
• 金额: $ 5万
• 依托单位: University of California-Santa Cruz
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-08-01 至 2023-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2225739&HistoricalAwards=false
• 关键词: Corps; Massively; Parallel; Resolution; Optical

The broader impact/commercial potential of this I-Corps project is the potential development of a system that could provide more cost-effective and easier-to-use tools for scientists in electrophysiology, and possibly allow the improved characterization of dense electrogenic cell cultures. The technology may allow pharmaceutical companies the ability to better understand how cells respond to new drugs and could provide more informed, data-driven decisions during clinical trials. This innovation could also provide biologic manufacturers with more information regarding the development of their cell cultures which may reduce production costs associated with failed cell cultures. In addition, academic scientists may utilize this technology to characterize information transfer in large neural populations and which may unlock new insights in fields such as neuroscience.This I-Corps project is based on the development of a novel system for phenotypic screening and monitoring in electrophysiology. The system monitors the electrical activity of cardiomyocytes, neurons, and other cells to characterize their behavior and health with potentially more spatial resolution, throughput, and monitoring time than other systems. This may allow drug development and biologics manufacturing to gather more data and develop more robust assays for their product pipelines. The current approach to monitoring cells for phenotypic screening involves the use of microelectrode arrays, which can be limited in spatial resolution, are comparatively low throughput, and may cost more for industries to utilize. This technology uses a novel optical sensor that combines plasmonics with electrochromics to possibly create a platform that would be wireless, high resolution, and high throughput for long-term monitoring of electrophysiology. This platform may enable the development of cheaper, more robust assays as well as entirely new characterizations of cell health and information exchange in cell populations.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

该I-Corps项目的更广泛的影响/商业潜力是系统的潜在开发，该系统可以为电生理学科学家提供更具成本效益，更易于使用的工具，并可能可以改善密集的电细胞培养物的表征。该技术可以使制药公司能够更好地了解细胞对新药的反应，并在临床试验期间提供更明智的，数据驱动的决策。这项创新还可以为生物制造商提供有关其细胞培养的更多信息，这些信息可能会降低与细胞培养失败相关的生产成本。此外，学术科学家可以利用这项技术来表征大型神经种群中的信息传递，并可以在神经科学等领域中释放新的见解。该I-Corps项目基于开发用于电生理学中表型筛查和监测的新型系统。该系统监视心肌细胞，神经元和其他细胞的电活动，以比其他系统的空间分辨率，吞吐量和监测时间更具空间分辨率，吞吐量和监测时间来表征其行为和健康。这可以使药物开发和生物制造制造能够收集更多数据并为其产品管道开发更多可靠的测定法。当前监测表型筛选细胞的方法涉及使用微电极阵列的使用，该阵列可以限制在空间分辨率方面，吞吐量相对较低，并且可能需要花费更多的行业。这项技术使用一种新型的光学传感器，将等离子原料与电染色体结合起来，可能创建一个平台，该平台将是无线，高分辨率和高通量，以长期监测电生理学。该平台可以使更便宜，更健壮的测定法以及细胞种群中细胞健康和信息交换的全新特征的开发。该奖项反映了NSF的法定任务，并被认为是通过基金会的智力优点和更广泛的影响来通过评估来获得支持的。