Collaborative Research: Mechanisms of pollen-specific phospholipases in maize haploid production

合作研究:花粉特异性磷脂酶在玉米单倍体生产中的机制

基本信息

  • 批准号:
    2222158
  • 负责人:
  • 金额:
    $ 55.41万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
    Standard Grant
  • 财政年份:
    2022
  • 资助国家:
    美国
  • 起止时间:
    2022-08-01 至 2025-07-31
  • 项目状态:
    未结题

项目摘要

Sexual reproduction mixes the genetic materials from two parents, which is the driving force for natural selections and a basis for plant breeding improvements. However, the cellular and molecular mechanisms that ensure the transfer of the genetic information from each parent to the next generation are largely unknown. This project addresses how specific enzymes that cleave phospholipids, which are building blocks of cell membranes, such as those of sperm and egg cells, affect the sperm genome transfer in maize seed production. Genetic defects in those enzymes lead to some embryos without the sperm genome, producing maternal haploids with genetic information only from the egg. This study will investigate how those enzymes affect membrane lipid changes and how the lipid changes lead to haploid formation. Thus, findings from this project will advance the fundamental, mechanistic understanding of sperm genome stability and haploid seed production with potential applications to crop improvement, such as haploid induction technology to enhance crop breeding and production for food, feed, fuel, and industrial feedstocks. This project will have additional broader impacts on science, education, and society by providing opportunities for training of students and postdoctoral researchers and serving as a platform to broaden participation of underrepresented groups in research. The research materials, including various maize lines, will be distributed to community and the results and large datasets will be disseminated to enhance science and education through lectures and seminars, in classroom teaching, at national and international meetings, and through timely publications in peer-reviewed journals.The goal of this project is to elucidate how two pollen-specific phospholipases, pPLAIIφ and PLD3, are involved in sperm genome transmission and haploid seed production in cereal crops. The research tests the hypotheses that loss of the pPLAIIφ and PLD3 activities perturbs lipid homeostasis, membrane structures, and sperm-egg membrane fusion during fertilization, leading to haploid induction and decreased seed production. The supporting objectives are to: 1) determine the role of pPLAIIφ and PLD3 catalytic activities in haploid and seed production, 2) characterize the effect of the phospholipases on pollen and sperm membrane lipid homeostasis, and 3) measure lipid dynamics and effects on sperm-egg fusion. The results will advance the understanding of sperm genome stability and sexual reproduction and have the potential to identify chemical and molecular modifiers applicable to enhance haploid induction for crop improvement.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.
有性繁殖将两个父母的遗传材料混合在一起,这是自然选择的驱动力,也是植物育种改善的基础。然而,确保遗传信息从每个父母转移到下一代的细胞和分子机制在很大程度上未知。该项目解决了清除磷脂的特定酶,这些酶是细胞机制的基础,例如精子和卵细胞的基础,会影响玉米种子生产中的精子基因组转移。这些酶中的遗传缺陷导致一些没有精子基因组的胚胎,仅产生卵子信息的母体单倍体。这项研究将研究这些酶如何影响膜脂质变化以及脂质变化如何导致单倍体形成。这是该项目的发现将提高对精子基因组稳定性和单倍体种子产生的基本,机械的理解,并具有潜在的作物改进应用,例如单倍体诱导技术,以增强农作物,饲料,饲料,燃料和工业饲料的作物繁殖和生产。该项目将通过为培训学生和博士后研究人员提供机会,并作为扩大代表性不足的研究团体参与研究的平台,从而对科学,教育和社会产生更广泛的影响。研究材料(包括各种玉米系列)将分配给社区,结果和大型数据集将被分散,以通过讲座和半座,在课堂教学,国家和国际会议上以及通过Peer评估的期刊中的及时出版物来增强科学和教育。谷物作物中的单倍种子产生。该研究检验了pplaiiφ和PLD3活性的丧失脂质稳态,膜结构和精子 - 蛋膜在受精过程中的丧失,从而导致单倍倍诱导并产生种子产生。支持对象是:1)确定pplaiiφ和PLD3催化活性在单倍体和种子产生中的作用,2)表征磷脂酶对花粉和精子膜脂质稳态的影响,以及3)测量脂质动力学和对精子融合的影响。结果将提高人们对精子基因组稳定性和有性繁殖的理解,并有可能识别适用于改善农作物改善单倍体诱导的化学和分子修饰符。该奖项反映了NSF的法定任务,并被认为是通过使用基金会的知识分子优点和更广泛的影响审查标准来通过评估来获得的珍贵支持。

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

暂无数据

数据更新时间:2024-06-01

Bing Yang其他文献

Protective effect of ferulic acid on human umbilical vein endothelial cell model of cold stress
阿魏酸对冷应激人脐静脉内皮细胞模型的保护作用
  • DOI:
    10.4103/pm.pm_631_18
    10.4103/pm.pm_631_18
  • 发表时间:
    2020-01
    2020-01
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Si Huang;Shengsuo Ma;Di Zhang;Bing Yang;Jianxin Sun;Meina Huang;Haoming Lin;Meijing Xie;Guoping Zhao
    Si Huang;Shengsuo Ma;Di Zhang;Bing Yang;Jianxin Sun;Meina Huang;Haoming Lin;Meijing Xie;Guoping Zhao
  • 通讯作者:
    Guoping Zhao
    Guoping Zhao
Polyaromatic hydrocarbons in biochars and human health risks of food crops grown in biochar-amended soils: A synthesis study
生物炭中的多环芳烃和生物炭改良土壤中种植的粮食作物对人类健康的风险:综合研究
  • DOI:
    10.1016/j.envint.2019.06.009
    10.1016/j.envint.2019.06.009
  • 发表时间:
    2019
    2019
  • 期刊:
  • 影响因子:
    11.8
  • 作者:
    Jian Wang;Emmanuel Stephen Odinga;Wei Zhang;Xian Zhou;Bing Yang;Michael Gatheru Waigi;Yanzheng Gao
    Jian Wang;Emmanuel Stephen Odinga;Wei Zhang;Xian Zhou;Bing Yang;Michael Gatheru Waigi;Yanzheng Gao
  • 通讯作者:
    Yanzheng Gao
    Yanzheng Gao
Preparation of SiO2-Cenoshpere Composites and Super-hydrophobic Surface
SiO2-空心珠复合材料及超疏水表面的制备
Secure Multi-Party Computing for Financial Sector Based on Blockchain
基于区块链的金融领域安全多方计算
Exploring the mechanism of action of Yiyi Fuzi Baijiang powder in colorectal cancer based on network pharmacology and molecular docking studies.
基于网络药理学和分子对接研究探讨薏苡附子白姜散治疗结直肠癌的作用机制。
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前往

Bing Yang的其他基金

Collaborative Research: RESEARCH-PGR: Development of epigenetic editing for crop improvement
合作研究:RESEARCH-PGR:用于作物改良的表观遗传编辑的开发
  • 批准号:
    2331438
    2331438
  • 财政年份:
    2024
  • 资助金额:
    $ 55.41万
    $ 55.41万
  • 项目类别:
    Standard Grant
    Standard Grant
Collaborative Research: RESEARCH-PGR: Genome-wide quest for non-host resistance mechanisms in plants
合作研究:RESEARCH-PGR:全基因组探索植物非宿主抗性机制
  • 批准号:
    2224204
    2224204
  • 财政年份:
    2023
  • 资助金额:
    $ 55.41万
    $ 55.41万
  • 项目类别:
    Standard Grant
    Standard Grant
RESEARCH-PGR: SECRETome Project: Systematic Evaluation of CellulaR ExporT from plant cells
RESEARCH-PGR:SECRETome 项目:植物细胞 CellulaR ExportT 的系统评估
  • 批准号:
    1936492
    1936492
  • 财政年份:
    2019
  • 资助金额:
    $ 55.41万
    $ 55.41万
  • 项目类别:
    Continuing Grant
    Continuing Grant
RESEARCH-PGR: SECRETome Project: Systematic Evaluation of CellulaR ExporT from plant cells
RESEARCH-PGR:SECRETome 项目:植物细胞 CellulaR ExportT 的系统评估
  • 批准号:
    1810468
    1810468
  • 财政年份:
    2017
  • 资助金额:
    $ 55.41万
    $ 55.41万
  • 项目类别:
    Continuing Grant
    Continuing Grant
Collaborative Research: The role of host nutrient carriers in pathogen susceptibility
合作研究:宿主营养载体在病原体易感性中的作用
  • 批准号:
    1258103
    1258103
  • 财政年份:
    2013
  • 资助金额:
    $ 55.41万
    $ 55.41万
  • 项目类别:
    Standard Grant
    Standard Grant

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  • 批准号:
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