MRI: Acquisition of A MALDI-TOF Mass Spectrometer for Accurate Mass Determination of Biomolecules

MRI：购买 MALDI-TOF 质谱仪以准确测定生物分子的质量

• 批准号: 2215917
• 负责人: Jinglin Fu
• 金额: $ 26万
• 依托单位: Rutgers University Camden
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-09-01 至 2023-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2215917&HistoricalAwards=false
• 关键词: MRI; Acquisition; MALDI; TOF; Mass

The significance of this proposed Major Research Instrumentation (MRI) project is to fill in the existing gap of lacking scientific equipment to advance multidisciplinary research and to promote educational and outreach activities in the South Jersey area. Multiple schools and institutes will be benefited from acquiring the equipment, including 16 key user labs from 7 different departments at Rutgers-Camden, Rowan University and the Joint Health Sciences Center (JHSC) in Camden. The potential impacts include the enhancement of STEM outreach for under-represented/minority students in the Camden area; advancement of classroom teaching, experiential learning and research training for undergraduates and graduate students; the promotion of collaboration within local schools and industrial partners. The proposed equipment will also interface with existing facilities to enhance the setup of a core facility of Scientific Measurements at Rutgers University-Camden. The success of instrumental use will be evaluated by the number of users, product of publications, conference presentations, grant awards, class participation, outreach activities and other scientific disseminations. This project serves the interest and mission of NSF to promote the progress of science; to advance the national health, prosperity and welfare; and to secure the national defense. The MALDI-TOF instrument is used for the analysis of various high molecular-weight biomolecules, such as nucleotides, peptides proteins and polysaccharides. The acquisition of this instrument will support multidisciplinary research programs and student education in the fields of biomimetic self-assembly, cellular biochemistry, protein chemistry, novel biomaterials, nanomaterials, forensic analysis and molecular sensing, as well as increasing knowledge of health-relevant mechanisms. Multiple research and outreach projects will be enhanced by this instrument, including: (1) Characterization of biomolecule modification and conjugation for developing biomimetic assemblies and smart reactors; (2) Development of biomaterials and biopolymers; (3) Nanoparticles synthesis, assembly and delivery; (4) Enhanced bimolecular characterization for studying cellular functions and activities; (5) Enhanced methods for forensic body fluid identification; (6) Characterization of peptides and proteins for bimolecular function; (7) Enhancement of research outreach and training.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

该提出的主要研究工具（MRI）项目的重要性是填补现有的缺乏科学设备来推进多学科研究并促进南泽西地区的教育和外展活动的空白。获取设备，包括来自Rutgers-Camden，Rowan University和Camden联合健康科学中心（JHSC）的7个不同部门的16个主要用户实验室，将受益于多个学校和学院。 潜在的影响包括对卡姆登地区代表性不足/少数族裔学生的STEM外展的增强；为大学生和研究生提供课堂教学，体验学习和研究培训的进步；促进当地学校和工业伙伴内的合作。拟议的设备还将与现有的设施进行交互，以增强罗格斯大学污染科学测量的核心设施。工具使用的成功将由用户数量，出版物的产品，会议演示，授予奖项，班级参与，外展活动和其他科学传播评估。该项目符合NSF促进科学进步的兴趣和使命。促进民族健康，繁荣和福利；并确保国防。 MALDI-TOF仪器用于分析各种高分子量生物分子，例如核苷酸，肽蛋白和多糖。该仪器的获取将支持仿生自组装，细胞生物化学，蛋白质化学，新型生物材料，纳米材料，法医分析和分子传感的多学科研究计划和学生教育，以及对健康相关机制的了解。该工具将增强多个研究和外展项目，包括：（1）生物分子修饰和共轭的表征，用于开发仿生组装和智能反应堆； （2）生物材料和生物聚合物的发展； （3）纳米颗粒合成，组装和输送； （4）增强的双分子表征研究细胞功能和活性； （5）增强的法医体液鉴定方法； （6）表征肽和蛋白质的双分子功能； （7）增强研究外展和培训。该奖项反映了NSF的法定任务，并通过使用基金会的知识分子优点和更广泛的影响审查标准来评估值得支持。

项目成果

Competitive aptamer switch for modulating ligand binding affinity

• DOI: 10.1016/j.aac.2023.06.004
• 发表时间: 2023-06
• 期刊: Advanced Agrochem
• 作者: Derek Puyat;Sung Won Oh;Shiming Liu;Jinglin Fu