Nonlinear Surface Spectroscopic Investigations into the Molecular Mechanism Governing Cell Membrane Repair

细胞膜修复分子机制的非线性表面光谱研究

• 批准号: 2137997
• 负责人: Andrew Carpenter
• 金额: $ 30万
• 依托单位: Carpenter, Andrew P
• 依托单位国家: 美国
• 项目类别: Fellowship Award
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-09-01 至 2024-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2137997&HistoricalAwards=false
• 关键词: Nonlinear; Surface; Spectroscopic; Investigations; into

Andrew P. Carpenter is awarded a FY21 Mathematical and Physical Sciences Ascending Postdoctoral Research (NSF MPS-Ascend) Fellowship to conduct a program of research and activities related to broadening participation by groups underrepresented in Chemistry at Oregon State University. The project being performed by Andrew Carpenter, entitled Nonlinear Surface Spectroscopic Investigations into the Molecular Mechanism Governing Cell Membrane Repair, is to be conducted under the guidance of mentoring scientist Joe Baio. This project is supported by the Office of Multidisciplinary Activities and the Chemistry Division in the Mathematical and Physical Sciences, and by the Molecular Biophysics Program of the Molecular and Cellular Biosciences Division of the Biological Sciences Directorate.Membrane-bound proteins carry out many cellular functions, including repair of tears in the cellular plasma membrane. This process is not well understood, and this project will utilize vibrational sum-frequency generation (VSF) spectroscopy to investigate the mechanism governing membrane repair by the specific protein dysferlin, including determining the phospholipids that dysferlin binds at membrane surfaces to initiate the membrane repair process, elucidating the membrane packing conditions necessary for dysferlin binding, directly interrogating dysferlin at vesicle surfaces using VSF scattering spectroscopy, and establishing experimental and computational methodology needed to extract structural information from VSF spectra of multi-domain proteins. Broadening participation of Native Americans in science, technology, math and engineering will be supported by partnering with efforts at Oregon State University that focus on providing research opportunities and targeted STEM outreach events for students and teachers from the local Chemawa Indian school.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

安德鲁·P·卡彭特 (Andrew P. Carpenter) 获得 2021 财年数学和物理科学提升博士后研究 (NSF MPS-Ascend) 奖学金，用于开展一项与扩大俄勒冈州立大学化学领域代表性不足的群体的参与相关的研究和活动计划。 该项目由安德鲁·卡彭特（Andrew Carpenter）执行，题为“非线性表面光谱研究控制细胞膜修复的分子机制”，将在指导科学家乔·拜奥（Joe Baio）的指导下进行。 该项目得到了多学科活动办公室和数学和物理科学化学部以及生物科学理事会分子和细胞生物科学部分子生物物理学项目的支持。膜结合蛋白具有许多细胞功能，包括细胞质膜撕裂的修复。 这一过程尚不清楚，该项目将利用振动和频发生（VSF）光谱来研究特定蛋白质 Dysferlin 控制膜修复的机制，包括确定 Dysferlin 在膜表面结合的磷脂以启动膜修复过程，阐明了 Dysferlin 结合所需的膜堆积条件，使用 VSF 散射光谱直接询问囊泡表面的 Dysferlin，并建立了从多域蛋白的 VSF 光谱中提取结构信息所需的实验和计算方法。俄勒冈州立大学将与俄勒冈州立大学合作，支持扩大美国原住民对科学、技术、数学和工程的参与，该大学致力于为当地 Chemawa 印第安学校的学生和教师提供研究机会和有针对性的 STEM 外展活动。该奖项反映了 NSF 的贡献法定使命，并通过使用基金会的智力价值和更广泛的影响审查标准进行评估，被认为值得支持。

项目成果

“Nonlinear” pursuit of understanding pollutant accumulation and chemistry at environmental and biological interfaces

– 非线性 – 追求理解环境和生物界面上的污染物积累和化学反应

• DOI: 10.1116/6.0003059
• 发表时间: 2023-09
• 期刊: Biointerphases
• 影响因子: 2.1
• 作者: Carpenter, Andrew P.;Golbek, Thaddeus W.
• 通讯作者: Golbek, Thaddeus W.

Choose your own adventure: Picosecond or broadband vibrational sum-frequency generation spectroscopy

选择您自己的冒险：皮秒或宽带振动和频发生光谱

• DOI: 10.1116/6.0001844
• 发表时间: 2022-05
• 期刊: Biointerphases
• 影响因子: 2.1
• 作者: Carpenter, Andrew P.;Baio, Joe E.
• 通讯作者: Baio, Joe E.

The dysferlin C2A domain binds PI(4,5)P2 and penetrates membranes

Dysferlin C2A 结构域结合 PI(4,5)P2 并穿透细胞膜

• DOI: 10.1016/j.jmb.2023.168193
• 发表时间: 2023-07
• 期刊: Journal of Molecular Biology
• 影响因子: 5.6
• 作者: Kwok, Ethiene;Otto, Shauna C.;Khuu, Patrisha;Carpenter, Andrew P.;Codding, Sara;Reardon, Patrick N.;Vanegas, Juan;Kumar, Tanushri M.;Kuykendall, Chapman J.;Mehl, Ryan A.;et al
• 通讯作者: et al

Comparative Thermodynamic and Structural Analysis of Polyfluorinated Dodecylphosphonic Acid Adsorption to Distilled and River Water Interfaces

多氟十二烷基膦酸在蒸馏水和河水界面吸附的比较热力学和结构分析

• DOI: 10.1021/acs.jpca.3c01487
• 发表时间: 2023-07
• 期刊: The Journal of Physical Chemistry A
• 作者: Carpenter, Andrew P.;White, Jade N.;Hasbrook, Annemarie;Reierson, Makenna;Baio, Joe E.
• 通讯作者: Baio, Joe E.

Orientation of the Dysferlin C2A Domain is Responsive to the Composition of Lipid Membranes

Dysferlin C2A 结构域的方向与脂膜的组成有关

• DOI: 10.1021/acs.jpcb.2c06716
• 发表时间: 2023-01
• 期刊: The Journal of Physical Chemistry B
• 作者: Carpenter, Andrew P.;Khuu, Patricia;Weidner, Tobias;Johnson, Colin P.;Roeters, Steven J.;Baio, Joe E.
• 通讯作者: Baio, Joe E.