NSF-ANR: REBIRTH: Understanding The Self-Assembly Of The Nervous System of Hydra Vulgaris

NSF-ANR：重生：了解寻常九头蛇神经系统的自组装

• 批准号: 2203119
• 负责人: Rafael Yuste
• 金额: $ 57万
• 依托单位: Columbia University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-03-01 至 2025-02-28
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2203119&HistoricalAwards=false
• 关键词: NSF; ANR; REBIRTH; Understanding; Self

To understand the physical and biological principles underlying the self-assembly of the nervous system of the fresh-water polyp Hydra vulgaris, the PIs propose a collaboration between theoreticians and experimentalists that will focus on this problem. The PIs will image the activity of every neuron and muscle cell during re-aggregation of Hydra, and analyze it with novel imaging, statistical and mathematical tools, correlating the emergence of phase transitions in the neuronal and muscle activity with the appearance of specific behaviors. This work will provide a deep insight into algorithms and mechanisms of self-assembly, with important repercussions for control theory, neuroscience, soft-matter physics, robotics and network science. Due to Hydra's extreme regenerative ability, this work may enable synthetic biology of an organism with a nervous system; and the work will have future repercussions for the field of neuroregeneration. The techniques developed for tracking of neurons in a moving and distorting animal will be made widely available and will be of use in other systems. The study of Hydra with the proposed integrated imaging/computational approach will serve as an imaginatively appealing platform for a range of outreach opportunities introducing members of the general public to mathematical neuroscience, and training opportunities for students at all levels. In particular, the Hydra system can be deeply integrated into the full spectrum of courses at the Marine Biological Laboratory and provide cross-cutting projects for students from diverse backgrounds.One of the most fascinating aspects of biological organisms is their self-assembly: bodies put themselves together without external directions, to yield robust, resilient and adaptive living systems. This is particular dramatic in the cnidarian Hydra vulgaris. This small, transparent polyp has unique regenerative properties, demonstrated in the ability to completely regenerate itself after its body has been dissociated into individual cells. This remarkable self assembly, regenerating a normal animal, is complete within a few days, occurs robustly in culture dishes under a microscope and has surprisingly not yet been studied systematically. This project goes to the heart of this problem and will apply the a state-of-the-art toolset of modern neuroscience and computational approaches to this fundamental problem.This collaborative US/France project is supported by the US National Science Foundation and the French Agence Nationale de la Recherche, where NSF funds the US investigator and ANR funds the partners in France.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

为了理解淡水息肉九头蛇神经系统的自我组装的基础的物理和生物学原理，PIS提出了理论家与实验家之间的合作，将重点放在这个问题上。 PI将在Hydra重新聚集过程中图像每个神经元和肌肉细胞的活性，并使用新颖的成像，统计和数学工具分析它，从而将神经元和肌肉活性中相变的出现与特定行为的出现相关联。这项工作将为自组装的算法和机制提供深入的见解，并对控制理论，神经科学，软性物理学，机器人技术和网络科学产生重要影响。由于Hydra的极端再生能力，这项工作可以使有机体具有神经系统的合成生物学。这项工作将对神经发生领域产生未来的影响。用于在动物和扭曲动物中跟踪神经元的技术开发的技术将被广泛使用，并将在其他系统中使用。通过拟议的综合成像/计算方法对HYDA进行的研究将成为一个具有富有吸引力的平台，以介绍一系列宣传机会，向公众介绍数学神经科学，并为各级学生提供培训机会。特别是，可以将Hydra系统深入整合到海洋生物实验室的全部课程中，并为来自不同背景的学生提供跨审计项目。一个生物生物的最迷人的方面之一是它们的自组装：身体是身体：身体放在一起，没有外部方向，可以产生强大的，弹性和适应性的生活系统。这在Cnidarian Hydra dulgaris中特别引人注目。这种透明的小息肉具有独特的再生特性，在将其身体分解为单个细胞后完全再生自身的能力证明。这种出色的自组装在几天之内完成，在显微镜下的培养皿中强烈地发生，令人惊讶的是尚未进行系统的研究。该项目成为这个问题的核心，并将应用现代神经科学和计算方法的最先进的工具集来解决这个基本问题。该协作的美国/法国项目得到了美国国家科学基金会和法国法国国家科学基金会和法国国民国家的支持，NSF资金在美国国家科学公司（NSF Nationale de la Recherche），美国国家科学基金会（NSF）基金在美国国家研究员和ANR基金宣布了诉讼。使用基金会的智力优点和更广泛的影响评估标准进行评估。