PFI-TT: Developing an integrated platform for high accuracy measurements of viral particle count and infectious titer

PFI-TT：开发用于高精度测量病毒颗粒计数和感染滴度的集成平台

• 批准号: 2141135
• 负责人: Sara Rouhanifard
• 金额: $ 24.98万
• 依托单位: Northeastern University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-02-01 至 2024-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2141135&HistoricalAwards=false
• 关键词: PFI; TT; Developing; integrated; platform

The broader impact/commercial potential of this Partnerships for Innovation - Technology Translation (PFI-TT) project is to measure viral concentrations in clinical or research settings. Select vaccines and gene therapies are delivered via viral vectors, but the precise dosing is poorly known, leading to quality control concerns. Current measurement techniques are indirect, imprecise, and nonstandard; and they require time and specialized equipment. Accurate measurement of viral particles is critical for precise benchmarking of diagnostics and appropriate dosing of therapeutics. This project develops a new technology to measure viral concentrations and infectivity. The proposed project addresses viral quantification techniques as they suffer from several limitations. Typical infectivity assays are highly dependent on the specific cell line used for viral infection, leading to high variability between labs. Furthermore, natural viruses are not engineered to contain a fluorescent transgene for easy identification, and some particles will not contain the viral genome - leading to competition between those containing the gene “payload” and empty vectors. In general, the density, infectivity, and replication efficiency of viral particles in a given sample is poorly known. This project advances a technology with three functioins: quantifying the total number of particles, measuring genome-containing units, and assessing infectivity of a given sample of the virus. The new method combines nano-pore technology using biophysical properties to identify viral particles with single-molecule RNA fluorescence in situ hybridization to discriminate down to single molecules of viral RNA in infected cells.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

创新 - 技术翻译（PFI -TT）离子在临床或研究环境中的更广泛的影响/商业潜力。和非标准的设备；使用的直线感染，导致天然病毒的差异很高，并没有发动机识别荧光透的识别，并且某些粒子将不包含病毒基因组，从而使基因“有效载荷”和一个空载体。病毒颗粒在给定样品中的效率很差。使用生物物理特性的纳米孔技术可以鉴定具有单分子原位杂交的病毒颗粒，以诊断为病毒RNA Infe in Infe Cyted细胞的诊断至单个分子。该奖项反映了NSF的捕获者，认为NSF值得在Thalugh thalugh的次数中使用ToughateTlectunectul a al sip and Broad Explys审查标准。