Elucidating the interplay between two chromatin regulators HDA8 and ELP3 in dynamic control of primary and secondary metabolic networks

阐明两个染色质调节因子 HDA8 和 ELP3 在初级和次级代谢网络动态控制中的相互作用

• 批准号: 2123470
• 负责人: Ying Li
• 金额: $ 100.2万
• 依托单位: Purdue University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-08-01 至 2024-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2123470&HistoricalAwards=false
• 关键词: Elucidating; interplay; between; two; chromatin

This project will yield knowledge on how plants coordinate metabolic networks to produce specialized metabolites that facilitate plant interactions with their environment, ranging from attracting pollinators and seed dispersers to protecting themselves from biotic and abiotic stresses. To date, it remains largely unknown how metabolic networks are regulated to balance the needs for maintaining essential functions and producing specialized metabolites. This project will shed light on chromatin regulatory mechanisms that coordinate metabolic networks to produce volatile secondary metabolites, by testing the hypothesis that an interplay between two chromatin regulators controls the dynamic metabolic flux through primary and secondary metabolic pathways. The obtained knowledge has practical implications for both agriculture and ecology, with potential to improve plant reproductive success, plant defense and stress responses, crop nutritional value, and yield of high value phytochemicals. This project will provide multidisciplinary training for the next generation of scientists, including minorities and high school students, in STEM research. Preliminary studies using Petunia hybrida flowers as a model system led to the hypothesis that Histone deacetylase 8 (PhHDA8) works with the histone acetyltransferase PhELP3 to regulate primary and secondary metabolic networks responsible for the biosynthesis and emission of volatile secondary compounds. To test this hypothesis, the following research objectives will be performed: (1) determining the impact of PhHDA8 and PhELP3 on dynamics of primary and secondary metabolic networks via metabolic profiling; (2) deciphering the genome-wide targets of PhHDA8 and PhELP3 in primary and secondary metabolic networks using functional genomics approaches; and (3) elucidating the molecular mechanisms coordinating PhHDA8 and PhELP3 activities through biochemical approaches. This work will provide important mechanistic insights into the mode of action and interaction of histone acetylation proteins. The obtained knowledge will have a far-reaching impact on understanding chromatin level regulation underlying the chemical diversity found in nature and airborne communications in the plants.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

该项目将产生有关植物如何协调代谢网络的知识，以产生促进植物与环境相互作用的专门代谢产物，从吸引传粉媒介和种子分散器到保护自​​己免受生物和非生物胁迫。迄今为止，在很大程度上还尚不清楚如何调节代谢网络，以平衡维持基本功能和产生专业代谢产物的需求。该项目将通过测试以下假设，即两个染色质调节剂之间的相互作用通过原代和二级代谢途径控制动态代谢通量，从而阐明了染色质调节机制，以协调代谢网络以产生挥发性次级代谢物。获得的知识对农业和生态学都具有实际意义，具有改善植物生殖成功，植物防御和压力反应，作物营养价值以及高价值植物化学物质的产量的潜力。该项目将为STEM研究中的下一代科学家（包括少数民族和高中生）提供多学科培训。使用矮牵牛杂种花作为模型系统的初步研究导致了这样一种假说，即组蛋白脱乙酰基酶8（PHHDA8）与组蛋白乙酰基转移酶Phelp3合作，用于调节负责挥发性化合物的生物合成和发射。为了检验这一假设，将执行以下研究目标：（1）通过代谢分析确定PHHDA8和PHELP3对主要和次级代谢网络动力学的影响； （2）使用功能基因组方法在原代和次级代谢网络中解密PHHDA8和PHELP3的全基因组靶标； （3）阐明通过生化方法协调PHHDA8和PHELP3活动的分子机制。这项工作将为组蛋白乙酰化蛋白的作用方式和相互作用提供重要的机理见解。所获得的知识将对理解自然界中的化学多样性和机载通信的基础化的染色质水平的调节产生深远的影响。该奖项反映了NSF的法定任务，并被认为值得通过基金会的知识分子优点和更广泛的影响审查标准通过评估来进行评估。