BII: Regional OneHealth Aerobiome Discovery Network (BROADN)

BII：区域 OneHealth 航空生物组发现网络 (BROADN)

• 批准号: 2120117
• 负责人: Sue VandeWoude
• 金额: $ 1250万
• 依托单位: Colorado State University
• 依托单位国家: 美国
• 项目类别: Cooperative Agreement
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-09-01 至 2026-08-31
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2120117&HistoricalAwards=false
• 关键词: BII; Regional; OneHealth; Aerobiome; Discovery

This project will explore fundamental details about microbes that live in the air, the aerobiome. The health of humans, other animals, and plants depends upon vast communities of microorganisms that colonize living surfaces. The same is true in soil and water, where microorganisms influence the health and productivity of those environments. In contrast, microbial life found in the atmosphere is understudied, even though we know that microorganisms can move through the air, serving vital ecological functions as well as providing disease transmission pathways. For example, many pathogens of animals and plants are spread via the air, and certain bacteria that are found in clouds are capable of influencing precipitation patterns. Key knowledge gaps about the aerobiome remain due to the technical challenges associated with studying this system. The interdisciplinary team constituting the Biology Integration Institute: Regional OneHealth Aerobiome Discovery Network (BROADN) will study how airborne microbial populations are altered by seasonality, weather patterns, and environmental stresses such as drought, intensive agriculture, and fire. This project will also investigate how microbes move between the land surface and the atmosphere, and what properties allow some organisms to survive in the atmosphere. Collaboration with regional partners, including a First Forward Institution serving rural first-generation students and a Hispanic Serving Institution will involve students in sample collection and analyses, revealing the fascinating unseen biology of the air. These and other educational programs will break down disciplinary barriers to educate the next generation of biologists and will share insights about the aerobiome to K-12 students and the public. The new understanding of the life cycles of microbes in the atmospheres is anticipated to lead to innovative solutions to key challenges such as the spread of pathogens, drought adaptation, and loss of terrestrial biodiversity. BROADN will partner with the Global Atmospheric Microbiome Project (GAMP), the NSF-sponsored National Ecological Observation Network (NEON), and the Joint Genome Institute (JGI) to organize rigorous atmospheric sampling of aerobiome states and fluxes, and to conduct bioinformatics analysis of the genetically diverse taxa that exist in the aerobiome. Focused regional aerobiome samples will be collected over time at different altitudes and coupled with detailed laboratory analyses and meteorological measurements. Environmental chambers will be used for specific hypothesis testing. Data generated will provide the basis for discoveries of aerobiome structure and function, with the resulting insight used to parameterize models that relate aerobiome dynamics to terrestrial ecosystem properties. Bioinformatics and transcriptomic analysis coupled with mathematical modeling will reveal the microbial composition of the aerobiome; its response to changes in weather patterns, anthropogenic disturbances, and physical forces; the characteristics that lead to lofting, transport, and survival of microbes; and the metabolic activity and genomic attributes of aerosolized microbes. Moreover, BROADN will provide international leadership in this emerging area, and set global standards for measurement and assessment of the aerobiome. This project will develop essential understanding to drive solutions to airborne transport of animal and plant pathogens, and to understand the ecological role of the aerobiome in preserving ecosystem health.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

该项目将探讨有关居住在空中（Aerobiome）中的微生物的基本细节。 人类，其他动物和植物的健康取决于在生物表面定居的广阔的微生物社区。在土壤和水中，微生物会影响这些环境的健康和生产力，也是如此。相比之下，即使我们知道微生物可以在空气中移动，提供重要的生态功能并提供疾病传播途径，但在大气中发现的微生物寿命已经进行了研究。例如，许多动物和植物的病原体都是通过空气传播的，并且在云中发现的某些细菌能够影响降水模式。由于与研究该系统相关的技术挑战，有关操纵组的关键知识差距仍然存在。构成生物融合研究所的跨学科团队：区域孤立的卫生局局体发现网络（BRODN）将研究空中微生物种群如何因季节性，天气模式和诸如干旱，强化农业和火灾等环境压力而改变。该项目还将调查微生物如何在陆地表面和大气之间移动，哪些特性允许某些生物在大气中生存。与区域合作伙伴的合作，包括为农村第一代学生和西班牙裔服务机构提供服务的第一家远期机构，将使学生参与样本收集和分析，揭示出令人着迷的空中未见生物学。这些和其他教育计划将打破纪律障碍，以教育下一代生物学家，并将向K-12学生和公众分享有关Ounobiome的见解。人们对大气中微生物的生命周期的新理解预计将导致创新的解决方案，以应对关键挑战，例如病原体，干旱适应和陆地生物多样性的丧失。 Broadn将与全球大气微生物组项目（GAMP），NSF赞助的国家生态观察网络（NEON）和联合基因组研究所（JGI）合作，以组织有机体状态和磁通状态的严格大气采样，并对存在于Aerobiome中的遗传学分类的生物信息学分析。随着时间的推移，将在不同的高度上收集重点的区域性飞行组样品，并与详细的实验室分析和气象测量相结合。环境室将用于特定的假设检验。生成的数据将为有机体组的结构和功能的发现提供基础，并将其用于参数化模型的洞察力将Aerobiome Dynamics与陆地生态系统属性相关联。生物信息学和转录组分析与数学建模相结合将揭示有机体的微生物组成。它对天气模式，人为障碍和身体力量变化的反应；导致微生物的隆起，运输和存活的特征；以及雾化微生物的代谢活性和基因组属性。此外，布罗德将在这个新兴领域提供国际领导，并将全球标准设定为衡量和评估 - 气候组。该项目将发展基本的理解，以推动动物和植物病原体的空降运输解决方案，并了解有机体在维护生态系统健康中的生态作用。该奖项反映了NSF的法定任务，并被认为是值得通过基金会的知识分子优点和更广泛影响的审查标准来通过评估来支持的。

项目成果

Contribution of soil bacteria to the atmosphere across biomes

• DOI: 10.1016/j.scitotenv.2023.162137
• 发表时间: 2023-02-13
• 期刊: SCIENCE OF THE TOTAL ENVIRONMENT
• 影响因子: 9.8
• 作者: Archer, Stephen D. J.;Lee, Kevin C.;Pointing, Stephen B.
• 通讯作者: Pointing, Stephen B.