SBIR Phase I: Microbiome for improving salt stress tolerance in crops

SBIR 第一阶段：提高作物耐盐胁迫能力的微生物组

• 批准号: 2035899
• 负责人: John Freeman
• 金额: $ 25.47万
• 依托单位: INTRINSYX BIO INC.
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-02-15 至 2022-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2035899&HistoricalAwards=false
• 关键词: SBIR; Phase; Microbiome; improving; salt

The broader impact/commercial potential of this Small Business Innovation Research (SBIR) Phase I project is to provide an innovative approach to improve crop tolerance using novel seed treatments. To feed the growing world population (estimated 9.8 billion by 2050), global food production must increase by 70%, while reducing impact on land use, the environment, and ecosystems. However, high salinity conditions and drought have reduced the potential yield of crops; furthermore, the excessive application of fertilizers is disrupting global nitrogen and phosphorus cycles, reducing biodiversity, and causing aquatic pollution. This project will develop a technology to promote crop growth through improved stress tolerance and nutrient acquisition. The adoption of more sustainable agricultural practices could help farmers in increasing their profitability and reduce costs. This project will offer an effective solution to 570 million farms around the world for food production. By improving crop yield, the project could help address the economic loss of more than $27 billion/year in the US due to unproductive farmland. The proposed project will generate innovative endophyte-based formulations for seed treatments to improve tolerance to abiotic stress. Seeds will be coated with a mixture of highly characterized safe (non-pathogenic) endophytic bacteria and yeast strains in formulation with selected osmoprotectants and prebiotics that improve the survivability and efficacy of the selected microbes. The bio-inoculants will improve crop tolerance to salinity stress while supporting nitrogen fixation and mineral nutrient acquisition. This project will validate the feasibility of this approach on treatments for selected vegetable seeds. First efforts will be dedicated to obtaining stable seed treatment formulations with a high shelf-life when used at scale in parallel with fungicide and insecticide treatments. The ability of bio-inoculant formulations to increase tolerance to salinity will be validated in the greenhouse. Finally, the performance of the most optimal formulation will be tested in a small field trial under high salinity conditions to validate its ability to increase crop yield and quality.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

这项小型企业创新研究（SBIR）I期项目的更广泛的影响/商业潜力是提供一种创新的方法来使用新型种子处理来提高作物耐受性。为了养活不断增长的世界人口（估计到2050年），全球粮食产量必须增加70％，同时减少对土地利用，环境和生态系统的影响。但是，高盐度条件和干旱降低了农作物的潜在产量。此外，过度施肥正在破坏全球氮和磷周期，减少生物多样性并引起水生污染。该项目将通过提高压力耐受性和养分获取来开发一种技术来促进作物的生长。采用更可持续的农业实践可以帮助农民提高盈利能力并降低成本。该项目将为全球5.7亿个农场提供有效的解决方案，用于粮食生产。通过提高农作物的产量，由于农田非生产力，该项目可以帮助解决美国/年超过270亿美元的经济损失。拟议的项目将产生创新的基于内生菌的制剂，以提高对非生物压力的耐受性。种子将用高度特征的安全（非致病性）内生细菌和酵母菌菌株的混合物与所选的渗透保护剂和益生元制成，从而提高所选微生物的生存能力和功效。生物隔离剂将提高作物对盐度胁迫的耐受性，同时支持氮固定和矿物营养的获取。该项目将验证这种方法对选定蔬菜种子的治疗方法的可行性。当与杀菌剂和杀虫剂处理并行使用时，将专门用于获得具有高保存期的稳定种子处理配方。在温室中，将验证生物交流剂提高盐度耐受性的能力。最后，最佳配方的性能将在高盐度条件下的小型野外试验中进行测试，以验证其提高农作物产量和质量的能力。该奖项反映了NSF的法定任务，并被认为是通过基金会的智力优点和更广泛的影响来通过评估来获得支持的。