SBIR Phase II: Development of a Novel Measurement Technology to Enable Longitudinal Multiomic Investigations of the Gut Microbiome

SBIR 第二阶段：开发新型测量技术以实现肠道微生物组的纵向多组学研究

• 批准号: 2314685
• 负责人: Dylan Nichols
• 金额: $ 97.68万
• 依托单位: BIOMESENSE, INC.
• 依托单位国家: 美国
• 项目类别: Cooperative Agreement
• 财政年份: 2024
• 资助国家: 美国
• 起止时间: 2024-03-15 至 2026-02-28
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2314685&HistoricalAwards=false
• 关键词: SBIR; Phase; II; Development; Novel

The broader impact of this Small Business Innovation Research (SBIR) Phase II project would be a significant increase the commercial, scientific, and human health potential of the microbiome field by enabling large-scale, dense longitudinal measurement and analysis of microbial RNA. This could enable: (1) a significant reduction in false discoveries caused by a lack of reproducible data; (2) a major cost reduction – ensuring these advancements are accessible to the entire industry; (3) high-frequency sampling, making time-series explorations routine; and (4) the creation of a large-scale database that contains both DNA and RNA data, which could lead to unprecedented discovery and validation of precision medicine biomarkers. Successful completion of this project could have a high likelihood of advancing the health and welfare of the American public, increasing the economic competitiveness of the United States healthcare and life sciences sector, and enhancing partnerships between academia and industry.The proposed project describes the development of an RNA feature to an existing microbiome measurement and analysis platform that consists of an automated sample collection system and an analytics engine for sequence data. Currently, the question of which genes are being expressed by gut microbes (which can be studied using RNA transcripts) is an extraordinarily promising research field for advancing the understanding of host-microbial interactions. This is because bacteria that make up a small amount of the gut microbiome can still influence the community dramatically through their gene expression. This can be overlooked by DNA sequence data, which only measures bacterial abundance. Despite this, RNA remains under-utilized, as RNA quickly degrades outside of the cell, and most laboratories lack the capability to measure RNA at any significant scale reliably. The main activities of this project will be (1) refining a process for RNA capture and storage, (2) integrating these into the automated hardware system, and (3) developing the methods and infrastructure for analysis of the resulting RNA datasets. This project will allow studies of gene expression in the gut microbiome to become commonplace, allowing researchers to discover more powerful clinically relevant biomarkers for precision medicine applications.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

这项小型企业创新研究（SBIR）II期项目的更广泛影响将大大提高微生物组领域的商业，科学和人类健康潜力，通过实现大规模，密集的纵向测量和微生物RNA的分析。这可以实现：（1）由于缺乏可重复的数据而引起的错误发现显着降低； （2）大幅降低成本 - 确保整个行业都能获得这些进步； （3）高频抽样，使时间序列探索常规； （4）创建包含DNA和RNA数据的大型数据库，这可能导致前所未有的发现和精密医学生物标志物的验证。该项目的成功完成可能很有可能提高美国公众的健康和福利，从而提高了美国医疗保健和生命科学领域的经济竞争力，并加强了学术界和行业之间的伙伴关系。拟议的项目将RNA的开发描述了RNA的开发，以对现有的微生物群体测量和分析式的smecorts semalts andy Symects进行自动分析和分析系统分析和分析的分析。当前，肠道微生物表达哪些基因的问题（可以使用RNA转录本进行研究）是一个非常有前途的研究领域，可以促进对宿主微生物相互作用的理解。这是因为构成少量肠道微生物组的细菌仍然可以通过其基因表达动态影响社区。这可以通过DNA序列数据来忽略这一点，该数据仅测量细菌的丰度。尽管如此，RNA仍未充分利用，因为RNA迅速在细胞外降解，并且大多数实验室缺乏可靠地测量RNA的能力。该项目的主要活动将是（1）完善RNA捕获和存储的过程，（2）将它们集成到自动硬件系统中，以及（3）开发用于分析所得RNA数据集的方法和基础架构。该项目将使肠道微生物组中基因表达的研究变得司空见惯，从而使研究人员能够发现更强大的临床相关生物标志物以进行精确医学应用。该奖项反映了NSF的法定任务，并通过使用基金会的知识分子优点和更广泛的影响评估标准来评估值得支持。