RAPID: Viral Particle Disrupting and Sequestering Polymer Materials applied to Coronaviruses

RAPID：用于冠状病毒的病毒颗粒破坏和隔离聚合物材料

• 批准号: 2030567
• 负责人: Dominik Konkolewicz
• 金额: $ 18.18万
• 依托单位: Miami University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-06-01 至 2022-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2030567&HistoricalAwards=false
• 关键词: RAPID; Viral; Particle; Disrupting; Sequestering

This is an NSF RAPID award in response to the 2020 CARES Act and is managed by the Polymers Program in the Division of Materials Research of the Directorate for Mathematical and Physical Sciences.PART 1: NON-TECHNICAL SUMMARYSince the first cases of coronavirus disease 2019 (COVID-19) appeared in late 2019, the disease has infected millions globally. The virus responsible for COVID-19 can stay active, capable of causing infections, on various surfaces for days, during which time indirect contact transmission could occur. Coronaviruses contain both a surface envelope of lipids and surface presented proteins which resemble spikes. Both of these features of the virus can be used to trap and destroy the viruses within synthetic materials. Synthetic polymer materials capable of inactivating and sequestering the virus causing COVID-19 will be developed in this project. These materials will form tough structures, with the materials containing synthetic and natural groups to both disrupt the lipid molecules on the surface of the virus and to bind and trap the coronavirus spike proteins. The polymers will form a tough network, ensuring the material performs for an extended period of time. This research involves design and synthesis of polymers as well as characterization and study of their mechanical properties and focuses on developing materials that could be adapted or coated onto existing high-touch surfaces. Additionally, the project will create publicly accessible virtual presentations and content on how polymer materials are critical for the health care industry and innovations in materials for biomedical applications. With the development of materials with excellent durability and robust ability to disrupt and trap the coronavirus, a reduction in COVID-19 infection by mitigating the indirect contact transmission mechanism is possible.PART 2: TECHNICAL SUMMARYSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2) exhibits active lifetimes of over 24 hours. This enables transmission to occur hours or days after a virus containing droplet is deposited from an infected individual. Materials that destroy the virus and sequester the virus to the surface could reduce the transmission rate of coronavirus disease 2019 (COVID-19). This project will develop virus trapping and disrupting tough networks which could be used to coat commonly encountered surfaces. The polymer materials will disrupt the lipid envelope of SARS-CoV-2 viral particles and bind the spike on the surface of SARS-CoV-2 with high affinity. Both purely synthetic materials as well as hybrid peptide/synthetic materials approaches will be investigated. The polymers will include tough network forming functionalities as well as peptide or synthetic polymers for both lipid envelope disruption and spike protein binding. The scientific focus of the project is to determine how a polymer material's microstructure and functionality impacts its ability to: form tough and mechanically robust networks; disrupt viral lipid envelopes; and immobilize SARS-CoV-2 through the surface spike proteins. A library of polymer materials containing distinct crosslink densities and macromolecular architectures will be used to determine how polymer structure impacts a material's mechanical property, lipid particle rupturing capability, and ability to bind to SARS-CoV-2 spike proteins. This will guide the design of materials for optimal mechanical performance and coronavirus disrupting capabilities, and will facilitate the design of surface coatings that can hinder indirect contact transmission with long lifetimes of the structures. To remotely engage with the public on the importance of polymer materials, a series of monthly YouTube videos will be developed to convey how polymer materials are critical to health and safety, highlighting developments in materials for healthcare and biomedical applications.This grant is being awarded using funds made available by the Coronavirus Aid, Relief, and Economic Security (CARES) Act supplement allocated to MPS.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

这是响应 2020 年 CARES 法案而设立的 NSF RAPID 奖项，由数学和物理科学理事会材料研究司的聚合物项目管理。第 1 部分：非技术性摘要自 2019 年第一例冠状病毒病（ COVID-19）于 2019 年底出现，该疾病已感染全球数百万人。导致 COVID-19 的病毒可以在各种表面上保持活跃并能够引起感染数天，在此期间可能会发生间接接触传播。冠状病毒含有脂质表面包膜和类似刺突的表面蛋白质。病毒的这两个特征都可以用来捕获和消灭合成材料中的病毒。该项目将开发能够灭活和隔离引起 COVID-19 的病毒的合成聚合物材料。这些材料将形成坚韧的结构，这些材料含有合成和天然基团，既能破坏病毒表面的脂质分子，又能结合和捕获冠状病毒刺突蛋白。聚合物将形成坚韧的网络，确保材料长时间发挥作用。 这项研究涉及聚合物的设计和合成以及其机械性能的表征和研究，重点是开发可以适应或涂覆到现有高接触表面上的材料。此外，该项目还将创建可公开访问的虚拟演示和内容，介绍聚合物材料对医疗保健行业的重要性以及生物医学应用材料的创新。随着具有优异耐用性和强大破坏和捕获冠状病毒能力的材料的开发，通过减轻间接接触传播机制来减少 COVID-19 感染是可能的。 第 2 部分：技术摘要严重急性呼吸综合征冠状病毒 2 (SARS-CoV- 2) 表现出超过 24 小时的有效寿命。这使得传播能够在受感染个体沉积含有病毒的飞沫数小时或数天后发生。消灭病毒并将病毒隔离在表面的材料可以降低 2019 年冠状病毒病 (COVID-19) 的传播率。该项目将开发病毒捕获和破坏坚韧的网络，可用于覆盖常见的表面。聚合物材料会破坏 SARS-CoV-2 病毒颗粒的脂质包膜，并以高亲和力结合 SARS-CoV-2 表面的刺突。将研究纯合成材料以及混合肽/合成材料方法。这些聚合物将包括坚韧的网络形成功能以及用于脂质包膜破坏和刺突蛋白结合的肽或合成聚合物。该项目的科学重点是确定聚合物材料的微观结构和功能如何影响其以下能力：形成坚韧且机械坚固的网络；破坏病毒脂质包膜；并通过表面刺突蛋白固定 SARS-CoV-2。包含不同交联密度和大分子结构的聚合物材料库将用于确定聚合物结构如何影响材料的机械性能、脂质颗粒破裂能力以及与 SARS-CoV-2 刺突蛋白结合的能力。这将指导材料的设计，以实现最佳的机械性能和冠状病毒破坏能力，并将促进表面涂层的设计，以阻碍间接接触传播并延长结构的使用寿命。为了让公众远程了解聚合物材料的重要性，我们将制作一系列每月 YouTube 视频，以传达聚合物材料对健康和安全的重要性，重点介绍医疗保健和生物医学应用材料的发展。这笔赠款是通过根据《冠状病毒援助、救济和经济安全 (CARES) 法案》补充规定分配给 MPS 的资金。该奖项反映了 NSF 的法定使命，并通过使用基金会的智力优势和更广泛的影响审查标准进行评估，被认为值得支持。

项目成果

SARS‐CoV ‐2 spike protein capture by peptide functionalized networks

• DOI: 10.1002/pol.20220539
• 发表时间: 2022-11
• 期刊: Journal of Polymer Science
• 影响因子: 3.4
• 作者: M. Rahman;Chamoni W. H. Rajawasam;Nethmi De Alwis Watuthanthrige;J. L. Sparks;R. Page;Dominik Konkolewicz-Dominik-Konkol

Network polymers incorporating lipid-bilayer disrupting polymers: towards antiviral functionality

结合脂质双层破坏聚合物的网络聚合物：实现抗病毒功能

• DOI: 10.1039/d2py00602b
• 发表时间: 2022
• 期刊: Polymer Chemistry
• 影响因子: 4.6
• 作者: Burridge, Kevin M.;Rahman, Monica S.;De Alwis Watuthanthrige, Nethmi;Gordon, Emma;Shah, Muhammad Zeeshan;Chandrarathne, Bhagya Madhushani;Lorigan, Gary A.;Page, Richard C.;Konkolewicz, Dominik
• 通讯作者: Konkolewicz, Dominik

Simple polymerization through oxygen at reduced volumes using oil and water

• DOI: 10.1002/pol.20210386
• 发表时间: 2021-06-21
• 期刊: JOURNAL OF POLYMER SCIENCE
• 影响因子: 3.4
• 作者: Burridge, Kevin M.;Watuthanthrige, Nethmi De Alwis;Konkolewicz, Dominik
• 通讯作者: Konkolewicz, Dominik