MRI: Acquisition of a State-of-the-Art Analytical Ultracentrifuge for Biomedical and Materials Research

MRI：购买最先进的分析超速离心机用于生物医学和材料研究

基本信息

批准号：
2018942
负责人：
Nancy Horton
金额：
$ 34.29万
依托单位：
University of Arizona
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
2020
资助国家：
美国
起止时间：
2020-09-01 至 2023-08-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=2018942&HistoricalAwards=false
关键词：
MRI Acquisition State Art Analytical

项目摘要

An award is made to the University of Arizona to support the purchase of an analytical ultracentrifuge (AUC). More than 20 laboratories will use the new AUC, with research programs spanning basic biology, chemistry, materials science, and even biomedical research. As part of the research effort, 235 students, including 22 postdoctoral, 68 graduate and 145 undergraduate students will benefit from training with the state-of-the-art instrumentation. The training opportunities will be extended to collaborating schools and institutions across the Southwest. The University of Arizona is a Hispanic Serving Institution and hosts many programs supporting diversity and inclusion of under-represented groups, many of which will benefit from training with the new instrument. The AUC will also be used in the undergraduate lab courses, where students will test hypotheses they generate for structure and function of various enzymes. In addition, a “hands-on” demonstration showing the basic principles of ultracentrifugation will be developed in the outreach course for delivery at schools throughout Arizona.Research that will be facilitated by the AUC includes the investigation of a new enzyme mechanisms involving filament formation, DNA binding by viral replicative proteins, protein-protein interactions in cell signaling, and liquid droplet formation by intrinsically disordered proteins. The AUC will facilitate a range of research projects by providing rapid and accurate assessment of ligand binding affinity, stoichiometry, and when mechanisms of inhibition involve alterations of quaternary structure. Chemical labeling (and completeness of labeling) of proteins can be detected, as well as any effects on quaternary structure, solubility, and aggregation. Aggregation is a major roadblock to many structural and functional studies, and the new AUC will be used to assess protein preparation stability and aggregation. The masses and polydispersity of lipid containing nanodiscs will be analyzed to understand the effect of chain and headgroup size, structure, and chemistry on lipid packing. Materials science research will also benefit from the analysis of nanoparticles made from tungsten, molybdenum, and titanium. Results from these studies will be disseminated through peer-reviewed journals, presentations at scientific meetings, and through classes and public outreach presentations.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

为Analytracentrifuge（AUC）颁发了一项奖励，其研究计划涵盖了基本的生物学，化学，材料科学，甚至是生物医学研究的一部分。学生将受益于培训的培训，供应遗嘱的遗嘱遗嘱学校和整个南部地区的机构是西班牙裔。使用新的仪器。由AUC进行的促进包括研究涉及形成的癌细胞机制，病毒复制蛋白Gnaling的DNA结合以及液滴格式素，通过提供一系列研究项目。涉及蛋白质标记的第四纪结构的改变，可以检测到 - 对许多结构和功能研究的影响将分析脂质的纳米盘，以了解链和化学对脂质堆积材料的影响，从而使tungsten，molybdenum和titanium的纳米颗粒的分析也受益通过同行评审的期刊，科学会议上的演讲进行了传播，尽管课程和公共Athis奖反映了使用Toundation的Revader的Suthy评估的NSF'Sf'Stututory对W的影响。