Collaborative Research: RoL: The evo-devo of male pregnancy and its effects on the brood pouch microbiome

合作研究：RoL：雄性妊娠的进化-发育及其对育儿袋微生物组的影响

• 批准号: 2015419
• 负责人: Adam Jones
• 金额: $ 58.62万
• 依托单位: Regents of the University of Idaho
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-08-01 至 2024-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2015419&HistoricalAwards=false
• 关键词: Collaborative; Research; RoL; evo; devo

The evolution of novel traits can change the way that organisms interact with their environments to survive, grow and reproduce. Deep knowledge of the underlying genes and developmental changes that underly most evolutionary innovations is sparse, as is understanding of the ecological consequences for both the organisms in which novel traits emerged and the organisms with which they interact in communities. A particular gap in understanding is how the evolution of novel traits influences the biodiversity of their associated microbial communities. This project will help fill this gap in our knowledge by studying a remarkable innovation – male pregnancy in seahorses, pipefish and seadragons. This project will include the creation of new genome sequences and detailed studies of the developmental genetic underpinnings of the embryo brooding structures that make male pregnancy possible. The consequences of pouch evolution on the complexity and function of the community microbes in the pouch will also be studied, as well as how this unique host-associated microbiota can affect the fitness of embryos in the pouch. This project will provide research training to high school students, teachers, and undergraduates from underrepresented groups through immersive outreach and targeted support programs. The project will also support training of the next generation of scientists via education of Ph.D. students and postdoctoral scholars. Outreach to general public will be accomplished through public talks and through creation of a museum exhibit on syngnathid biology paired with web resources to support K-12 education.Male pregnancy, accompanied by morphologically diverse embryo brooding structures, is a defining evolutionary innovation in syngnathid fishes. The goal of this project is to build an integrative understanding of the developmental genetic origin of this remarkable syngnathid novelty and its role in mediating multi-level ecological interactions with host-associated microbiota. This project will include production of 19 new annotated reference genomes strategically sampled across the syngnathid lineage, morphogenetic analysis and transcriptional/epigenetic profiling of the developing pouch in a comparative framework that leverages the repeated, independent evolution of complex brooding structures in the family, and analysis of brood pouch biocomplexity as a determinant of pouch-associated microbiome assembly. When complete, this project will provide novel insights into genome structural evolution in syngnathids, identify protein sequence and gene regulation changes involved in brood pouch development, and address whether the evolution of the brooding tissues created specialization in host regulation of microbiota with consequences for brooded progeny. The work will attract new researchers to syngnathids for studies of evolutionary innovation and diversification. The project will provide research training to high school students, teachers, and undergraduates from underrepresented groups, and will support education of graduate students and postdoctoral scholars, including the opportunity to take intense short courses to learn next generation sequencing, bioinformatics, complex statistical analyses, and genome editing. Educational outreach to general public will be accomplished through public lectures by the PIs, and through creation of a museum exhibit on syngnathid biology, which will be paired with an associated web resource directed toward K-12 education.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

新特征的进化可以改变生物体与其环境相互作用以生存、生长和繁殖的方式。对大多数进化创新背后的潜在基因和发育变化的深入了解很少，对这两种生物体的生态后果的了解也很少。其中新特征的出现以及它们在群落中相互作用的生物体的一个特殊空白是新特征的进化如何影响其相关微生物群落的生物多样性，该项目将通过研究一个显着的特征来帮助填补我们的知识空白。创新——海马、海龙的雄性怀孕该项目将包括创建新的基因组序列，并对使雄性怀孕成为可能的胚胎育雏结构的发育遗传基础进行详细研究。育儿袋进化对育儿袋中微生物的复杂性和功能的影响。还研究了这种独特的宿主相关微生物群如何影响育儿袋中胚胎的健康状况。该项目将通过沉浸式方式为代表性不足群体的高中生、教师和本科生提供研究培训。该项目还将通过公开演讲和举办有关合颌类生物学的博物馆展览来支持对公众的教育，从而支持下一代科学家的培训。与网络资源相结合，支持 K-12 教育。雄性怀孕，伴随着形态多样的胚胎育雏结构，是合颌鱼类的一项决定性的进化创新。该项目的目标是建立对发育遗传起源的综合理解。这一非凡的合颌类新颖性及其在介导与宿主相关微生物群的多层次生态相互作用中的作用该项目将包括生产 19 个新注释的参考基因组，这些基因组在合颌类谱系中进行战略性采样，并对发育中的袋进行形态发生分析和转录/表观遗传分析。一个比较框架，利用家庭中复杂育雏结构的重复、独立进化，并分析育雏袋生物复杂性作为决定因素完成后，该项目将为合颌动物的基因组结构进化提供新的见解，识别育雏袋发育中涉及的蛋白质序列和基因调控变化，并解决育雏组织的进化是否产生了宿主调控的专业化。这项工作将吸引新的研究人员对合胞类动物进行进化创新和多样化的研究。该项目将为高中生、教师和本科生提供研究培训。代表性不足的群体，并将支持研究生和博士后学者的教育，包括参加密集的短期课程以学习下一代测序、生物信息学、复杂的统计分析和基因组编辑的机会。将通过公开讲座来完成对公众的教育推广。 PI，并通过创建关于合颌类生物学的博物馆展览，该展览将与针对 K-12 教育的相关网络资源配对。该奖项反映了 NSF 的法定使命，并通过使用基金会的智力价值和更广泛的影响审查标准。