Collaborative Research: RoL-Rules for Dynamic-Light Environmental Sculpting of Genomes

合作研究：基因组动态光环境塑造的 RoL 规则

• 批准号: 2034929
• 负责人: Corbin Jones
• 金额: $ 121.26万
• 依托单位: University of North Carolina at Chapel Hill
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-06-15 至 2025-05-31
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2034929&HistoricalAwards=false
• 关键词: Collaborative; Research; RoL; Rules; Dynamic

The problem of getting a clear signal in a noisy environment is a problem that not only humans must resolve but one that all organisms in nature experience. The goal of this research is to determine the rules by which plants respond to noisy and variable light signals. The investigators hypothesize that switching between low and high levels of light over varying periods of time changes how light is detected by plants. To study this problem, grasses were chosen because grasses evolved to grow in many types of light environments such as on the forest floor where light flickering and shadows are common to open fields where the light changes very little except for clouds and time of day. The investigators hypothesize that the ability for grasses to thrive in these different light environments is encoded in their genomes. The project will examine how genomically encoded sensing adaptations to dynamic and constant light environments shapes the physiology, development, genetics, and evolution of grasses. Investigators will cross train graduate students and post-docs, especially those from underrepresented groups in state-of-the-art molecular biology, physiology and genomics. Seminars will bring together internationally recognized experts in plant ecology, evolutionary biology, plant physiology and genetics with students and scientists who want to apply these perspectives to understand how plants respond to dynamic environments. Up to 60 new post-baccalaureate analysts will participate in international training workshops. Changes in genomic elements at several scales of organization that increase the fitness of an individual are maintained while those changes that decrease fitness are counter selected for propagation. Genomic elements that are neutral to fitness are filtered away relative to the energy cost of maintaining them. These rules are well established at the scale of genes but not yet scalable to genomes. This project takes an integrative approach to this question by characterizing the full genomes of a phenotypically diverse, yet monophyletic, set of grasses from Ecuador. These grasses live in diverse habitats ranging from the Galapagos Islands, the Paramo’ (alpine tundra), the rain forest, and riparian and littoral zones. These grasses also have a wide range of habits (tillering vs vines vs. bushy vs. tree-like), autotrophic metabolism (C3 vs. C4 carbon fixation), and survival strategies (perennial vs. annual). The specific question to be addressed is what genes/alleles shape morphologies/physiologies expressed in dynamic light environments as opposed to stable light environments. This project brings together two basic disciplines: botany and genomics but extends the connections into cell biology, developmental biology, evolution, and systematics.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

在嘈杂的环境中获得清晰信号的问题不仅是人类必须解决的问题，也是自然界所有生物都必须解决的问题。这项研究的目标是确定植物对嘈杂和可变光信号的反应规则。研究人员发现，在不同时间段内，低光和强光之间的切换会改变植物检测光的方式。为了研究这个问题，研究人员选择了草，因为草进化到可以在多种类型的光环境中生长，例如在森林地面上。在光线闪烁和阴影常见的地方打开探索者们发现，除了云层和一天中的时间外，草类在这些不同的光环境下生长的能力是在它们的基因组中编码的，该项目将研究基因组编码如何适应动态和恒定的光环境。研究人员将交叉培训研究生和博士后，特别是来自最先进的分子生物学、生理学和基因组学领域代表性不足的群体。国际公认的植物生态学、进化生物学、植物生理学和遗传学专家以及希望运用这些观点来了解植物如何应对动态环境的学生和科学家将参加国际培训研讨会。增加个体适应度的多个组织尺度的基因组元素被保留，而那些降低适应度的变化被反选择用于传播，相对于维持它们的能量成本，过滤掉对适应度中性的基因组元素。良好建立于该项目通过描述来自厄瓜多尔的一组表型多样但单系的草的完整基因组来解决这个问题，这些草生活在从加拉帕戈斯群岛到不同的栖息地。 、帕拉莫（高山苔原）、雨林、河岸和沿海地区这些草也有各种各样的习性（分蘖与藤蔓）。与丛生与树状）、自养代谢（C3 与 C4 碳固定）和生存策略（多年生与一年生）需要解决的具体问题是基因/等位基因塑造动态光下表达的形态/生理学。该项目汇集了两个基本学科：植物学和基因组学，但将联系扩展到细胞生物学、发育生物学、进化论和系统学。该奖项是 NSF 的法定使命，并被认为值得支持。通过使用基金会的智力优点和更广泛的影响审查标准进行评估。

项目成果

Seedling Chloroplast Responses Induced by N -Linolenoylethanolamine Require Intact G-Protein Complexes

N-亚麻酰乙醇胺诱导的幼苗叶绿体反应需要完整的 G 蛋白复合物

• DOI: 10.1104/pp.19.01552
• 发表时间: 2020
• 期刊: Plant Physiology
• 影响因子: 7.4
• 作者: Yan, Chengshi;Cannon, Ashley E.;Watkins, Justin;Keereetaweep, Jantana;Khan, Bibi Rafeiza;Jones, Alan M.;Blancaflor, Elison B.;Azad, Rajeev K.;Chapman, Kent D.
• 通讯作者: Chapman, Kent D.

Towards resolution of a paradox in plant G-protein signaling

• DOI: 10.1093/plphys/kiab534
• 发表时间: 2022-02-04
• 期刊: PLANT PHYSIOLOGY
• 影响因子: 7.4
• 作者: Ghusinga, Khem Raj;Elston, Timothy C.;Jones, Alan M.
• 通讯作者: Jones, Alan M.

Molecular switch architecture determines response properties of signaling pathways

• DOI: 10.1073/pnas.2013401118
• 发表时间: 2021-03-16
• 期刊: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• 影响因子: 11.1
• 作者: Ghusinga, Khem Raj;Jones, Roger D.;Elston, Timothy C.
• 通讯作者: Elston, Timothy C.