CAS: Sustainable Analytical Chromatography-Mass Spectrometry for Biological Compounds

CAS：生物化合物的可持续分析色谱-质谱法

• 批准号: 2003648
• 负责人: Susan Olesik
• 金额: $ 46.63万
• 依托单位: Ohio State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-08-15 至 2024-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2003648&HistoricalAwards=false
• 关键词: CAS; Sustainable; Analytical; Chromatography; Mass

With support from the Chemical Measurement and Imaging Program in the Division of Chemistry, Professor Susan Olesik and her group at Ohio State University are working to improve the capabilities of powerful analytical methods that combine separation of complex mixtures (using chromatography) and detection of the components in those mixtures (using mass spectrometry). Specifically, they seek to improve the performance of both elements by adding liquefied carbon dioxide to the solvents used for separation. The Olesik group is engaged in fundamental studies aimed not only improving performance, but also reducing the amount of sample and solvent needed for the analysis. The insights gained should enable new capabilities in a broad range of fields, such as environmental and biomedical studies. Graduate and undergraduate students involved in the project gain state-of-the arts skills that will prepare them for STEM careers. To better inform the general public about the importance of sustainability, online presentations are being developed to teach nonscience undergraduates about the properties, use, and sustainability of solvents. The presentations highlight the widespread public uses of solvents and the associated environmental, health and safety risks. The expectation is that better and more widespread understanding of those risks can reduce their impact.The Olesik group is using biodegradable enhanced-fluidity mobile phases generated from biomass (e.g., water-methanol-CO2) for separation and mass spectrometric detection of biologically relevant compounds. These mobile phases will yield both higher sensitivity and enhanced sustainability. The approach can serve a wide range of applications involving analyses of many polar compounds. Proteins are used as model analytes because of the significant use of chromatography-mass spectrometry for their characterization and the need for improved analytical performance metrics for these compounds. Insights from the charge state distributions of representative proteins should provide evidence on the protein conformation while in enhanced-fluidity liquids. Through studies of relative ionization efficiencies and ion internal energies, mechanistic insights on the electrospray ionization process when using enhanced-fluidity solvent mixtures are anticipated.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

在化学分区的化学测量和成像计划的支持下，苏珊·奥尔西克（Susan Olesik）和她在俄亥俄州立大学的小组正在努力提高强大的分析方法的能力，这些分析方法结合了复杂混合物的分离（使用色谱法）和这些混合物中成分的检测（使用质谱）。 具体而言，他们试图通过在用于分离的溶剂中添加液化二氧化碳来提高这两个元素的性能。 Olesik小组从事基础研究不仅旨在提高性能，还旨在减少分析所需的样品和溶剂量。 获得的见解应在环境和生物医学研究等广泛领域中实现新能力。 参与该项目的研究生和本科生获得了最先进的艺术技能，这将为STEM职业做好准备。 为了更好地告知公众可持续性的重要性，正在开发在线演示文稿，以教导非科学本科生有关溶剂的特性，使用和可持续性。演讲突出了公众广泛使用溶剂以及相关的环境，健康和安全风险。 期望的是，对这些风险的更好，更广泛的了解可以降低其影响。Olesik组使用了可生物降解的增强的增强的浮动性移动阶段（例如，水 - 甲醇-CO2），用于分离和质谱检测生物学相关化合物。 这些手机阶段将产生更高的灵敏度和增强的可持续性。该方法可以提供广泛的应用，涉及许多极性化合物的分析。 蛋白质被用作模型分析物，因为色谱质量光谱法对其表征进行了大量使用，并且需要改善这些化合物的分析性能指标。 来自代表性蛋白质的电荷状态分布的见解应提供有关蛋白质构象的证据，同时在增强液体液体中。通过研究相对电离效率和离子内部能量，预计使用增强的液化性溶剂混合物时，对电喷雾电离过程的机理见解。该奖项反映了NSF的法定任务，并被认为是通过基金会的智力优点和广泛的影响来评估Criteria的智力优点和广泛的criteria，并被视为值得通过评估的支持。