Mechanisms of Transport Through Plasmodesmata

通过胞间连丝的运输机制

• 批准号: 1930101
• 负责人: David Jackson
• 金额: $ 72.82万
• 依托单位: Cold Spring Harbor Laboratory
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-08-01 至 2023-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1930101&HistoricalAwards=false
• 关键词: Mechanisms; Transport; Through; Plasmodesmata

Living organisms are made up of smaller units called cells, and these cells need to communicate with each other to coordinate how they grow and develop. Plants have evolved a way to communicate by making tiny channels to connect their cells. Many different types of molecules pass through these channels to feed growing tissues and to instruct the plant how to grow. This project aims to understand how the passage of signals through the channels is controlled, by studying a newly discovered factor that controls this process. It will also screen for new factors that influence the way in which the channels instruct the growth of special types of cells called stem cells that are essential for plant growth. These studies have the potential to make significant improvements to agricultural productivity, and to limit the spread of plant diseases, which sometimes use the channels to spread through the plant. In addition to the scientific and technological advances detailed above, this project will train young scientists at various levels, as well as developing resources to involve high school students in cutting edge biology research. The PI directs the Partners For the Future Program at CSHL, which immerses local high school students in active research at Cold Spring Harbor Laboratory. He will also develop an educational exchange with an all-female minority serving high school in Brooklyn, New York. This activity will expose the excitement and applications of molecular biology to students who otherwise have little exposure to scientific research.Plasmodesmata are microscopic channels that connect plant cells to integrate growth, development and nutrient availability, providing organism wide connectivity. Plant development relies on pluripotent stem cells in specialized niches called meristems, and the movement, or "trafficking", of homeodomain transcription factors through plasmodesmata is required to maintain the stem cells. This research will develop and adapt state of the art methods in protein-RNA interactions, RNA localization and proteomic analyses to study a newly identified RNA binding protein that is required for trafficking of stem cell regulatory proteins. It will also test the hypothesis that the newly identified protein interacts in a protein-mRNA complex to facilitate passage of transcription factors through the plasmodesmata. In an independent approach, the research will also use a proteomic screen to identify new factors that control PD trafficking. Movement of protein and RNA signals in plants is critical for their development, as well as how plants respond to the environment. The results of this project could therefore allow the manipulation of plants to improve agricultural productivity. The project will also integrate training of junior scientists, including minority high school students, in molecular genetics research.This award was co-funded by the Physiological Mechanisms and Biomechanics Program in the Division of Integrative Organismal Systems and the Cellular Dynamics and Function Cluster in the Division of Molecular and Cellular Biosciences.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

活生物体由称为细胞的较小单元组成，这些细胞需要相互通信以协调它们的生长和发展方式。植物通过建立微小的通道连接其细胞来发展一种通信的方式。许多不同类型的分子穿过这些通道以喂养生长的组织并指导植物如何生长。该项目旨在通过研究控制此过程的新发现的因素来了解信号通过渠道的通过如何控制。它还将筛选出影响渠道指导特殊类型的称为干细胞生长的特殊类型的细胞生长的新因素，这对于植物生长至关重要。这些研究有可能对农业生产率进行重大改善，并限制植物疾病的传播，这些植物疾病有时会使用通道通过植物传播。除了上面详述的科学和技术进步外，该项目还将在各个层面上培训年轻科学家，并开发资源，使高中生参与尖端生物学研究。 PI指示CSHL的未来计划的合作伙伴，该计划使当地的高中学生在冷泉港实验室中积极研究。 他还将与纽约布鲁克林的全女性少数民族服务。这种活动将使分子生物学的兴奋和应用在否则几乎没有科学研究的学生身上。质量流质是微观通道，可将植物细胞连接起来以整合生长，发育和养分的可用性，从而提供有机体广泛的连接性。植物发育依赖于称为分生组织的专门壁ches中的多能干细胞，并且需要通过疟原虫转录因子的运动或“运输”来维持干细胞。这项研究将在蛋白质RNA相互作用，RNA定位和蛋白质组学分析中发展和适应最先进的方法，以研究一种新鉴定的RNA结合蛋白，这是对干细胞调节蛋白运输所必需的。它还将检验以下假设：新近鉴定的蛋白质在蛋白质-MRNA复合物中相互作用，以促进转录因子通过质量症。 在独立的方法中，该研究还将使用蛋白质组学筛选来确定控制PD运输的新因素。 植物中蛋白质和RNA信号的运动对于它们的发育以及植物对环境的反应至关重要。因此，该项目的结果可以使植物的操纵能够提高农业生产力。该项目还将在分子遗传学研究中整合初级科学家（包括少数高中生）的培训。该奖项是由生理机制和生物力学计划共同资助了综合有机体系统部门的生理机制和生物力学计划，以及细胞动力学和功能集群，在分子和细胞生物学奖中通过nsf的基础进行了统治。优点和更广泛的影响审查标准。

项目成果

An Aminoacyl tRNA Synthetase, OKI1, Is Required for Proper Shoot Meristem Size in Arabidopsis

• DOI: 10.1093/pcp/pcz153
• 发表时间: 2019-11-01
• 期刊: PLANT AND CELL PHYSIOLOGY
• 影响因子: 4.9
• 作者: Kitagawa, Munenori;Balkunde, Rachappa;Jackson, David
• 通讯作者: Jackson, David

An Optimized Whole‐Mount Immunofluorescence Method for Shoot Apices

一种优化的芽尖整体免疫荧光法

• DOI: 10.1002/cpz1.101
• 发表时间: 2021
• 期刊: Current Protocols
• 作者: Tran, Thu M.;Demesa‐Arevalo, Edgar;Kitagawa, Munenori;Garcia‐Aguilar, Marcelina;Grimanelli, Daniel;Jackson, David
• 通讯作者: Jackson, David

An RNA exosome subunit mediates cell-to-cell trafficking of a homeobox mRNA via plasmodesmata

• DOI: 10.1126/science.abm0840
• 发表时间: 2022-01-14
• 期刊: SCIENCE
• 影响因子: 56.9
• 作者: Kitagawa, Munenori;Wu, Peipei;Jackson, David
• 通讯作者: Jackson, David

Rapid expression of COVID-19 proteins by transient expression in tobacco.

通过在烟草中瞬时表达来快速表达 COVID-19 蛋白。

• 发表时间: 2020
• 期刊: bioRxiv
• 作者: Lindsay, P;Ackerman, A;Jian, Y;Artz, O;Rosado, D;Skopelitis, T;Kitagawa, M;Pedmale, UV;Jackson, D.
• 通讯作者: Jackson, D.