Collaborative Research: NCS-FR: Shedding light on brain circuits mediating navigation of the odor plume in a natural environment

合作研究：NCS-FR：揭示自然环境中介导气味羽流导航的大脑回路

• 批准号: 1926747
• 负责人: Ioannis Kymissis
• 金额: $ 35.62万
• 依托单位: Columbia University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-09-15 至 2024-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1926747&HistoricalAwards=false
• 关键词: Collaborative; Research; NCS; FR; Shedding

Animals have a keen ability to find odor sources such as food, partners, pups and predators through the sense of smell in a manner that cannot be replicated by machines. How brains mediate navigation of the environment (the odor plume) through smell is an important unsolved problem. Indeed there are many instances where using machines to navigate an odor landscape is an unmet need to society. For example, even though their training requires lengthy one on one interaction with a trainer, dogs are still used to find explosives in airports and in the battlefield. Our multidisciplinary Odor Plume Neurophotonics (OPeN) team will tackle understanding the brain circuits mediating odor plume navigation. This is a daunting task because it involves characterizing how odors diffuse in the air (environmental engineering), developing advanced miniature microscopes to record from the brain of freely moving animals (electrical and mechanical engineering), recording from brain regions processing odor plume information in real time (systems neuroscience and integrative neurophotonics), and developing mathematical procedures to quantify how the information necessary for successful odor plume navigation is represented in the brain (applied mathematics). Our team will engineer a novel miniature microscope to record activity as mice navigate the odor plume and will assess how the activity of these neurons result in successful odor plume navigation. Furthermore, the team members of OPeN are thoroughly committed to foster advancement of women, underrepresented minorities, veterans and disabled individuals in science and participate in various programs to promote science diversity. Additionally, the team members have a track record of disseminating their work and have an established partnership with a local small business, Intelligent Imaging Innovation, Inc. with world headquarters located in Denver. We will continue our commercial dissemination efforts of the technology developed in this project. Finally, we will endeavor to communicate science to the broader audience through venues such as Scientific American, Public Broadcasting Service and outreach through the Denver Museum of Nature and Science.Members of our OPeN interdisciplinary team developed a novel two photon fiber-coupled microscope for 3D imaging of brain activity in the freely moving mouse and generated and quantified realistic odor environments in the laboratory to explore algorithms used for odor-guided navigation. In this project we leverage the extensive expertise and achievements of the team to crack the circuit basis for odor plume navigation. We will develop a low-weight, miniature 3-photon fiber coupled microscope (3P-FCM) to record neuronal activity simultaneously in one brain area in two planes of view. In addition, OPeN will develop a portable photoionization (PID) sensor to detect the odorant concentration at the nostril as the animal navigates the odor plume. Members of the OPeN team will record neural activity in the hippocampus and cerebellum of animals navigating the odor plume and will develop a Bayesian analysis method to decode odor plume navigation from neural activity. This multidisciplinary approach will result in understanding of the brain mechanisms of odor plume navigation.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

动物具有敏锐的能力，可以通过无法通过机器复制的方式来找到食物，伴侣，幼崽和捕食者等气味。大脑如何通过气味介导环境导航（气味羽流）是一个重要的未解决问题。确实，在许多情况下，使用机器来导航气味景观是对社会的未满足需求。例如，即使他们的训练需要与教练的一对一互动，但仍被用来在机场和战场上找到炸药。我们的多学科气味羽毛神经传播（OPEN）团队将解决了解介导异味羽流导航的大脑电路。这是一项艰巨的任务，因为它涉及表征气味如何在空气中弥漫（环境工程），开发高级微型显微镜，以记录自由移动动物的大脑（电气和机械工程）的大脑，从大脑区域记录从大脑区域记录，从而处理气味羽状信息，从而在实时进行无效和整合的iSifition of Mative of Mative of Mative Introfices，并在数学上进行量化，以获取数学的信息，以获取数学的范围。大脑（应用数学）。当小鼠导航气味时，我们的团队将设计一个新颖的微型显微镜，以记录活动，并评估这些神经元的活动如何导致气味羽流导航。此外，公开赛的团队成员彻底致力于促进妇女，代表性不足的少数民族，退伍军人和残障人士的发展，并参与各种计划以促进科学多样性。此外，团队成员还有传播工作的记录，并与当地的小型企业，智能成像创新公司建立了合作伙伴关系。我们将继续在该项目中开发的技术的商业传播工作。最后，我们将努力通过丹佛自然与科学博物馆，通过科学美国，公共广播服务和外展等场所与更广泛的受众传达科学。我们开放的跨学科团队的成员开发了一个新颖的两个光子光纤耦合的显微镜 导航。在这个项目中，我们利用团队的广泛专业知识和成就来破解气味羽流导航的电路基础。我们将开发低重量的3光子纤维耦合显微镜（3P-FCM），以在两个视线平面中的一个大脑区域同时记录神经元活动。此外，开放式将开发便携式光电离（PID）传感器，以检测鼻孔的气味浓度，因为动物导航气味。开放团队的成员将记录海马和小脑的神经活动，这些动物会导航气味羽流，并将开发一种贝叶斯分析方法，以解释神经活动中的气味羽流导航。这种多学科方法将导致了解气味羽流导航的大脑机制。该奖项反映了NSF的法定任务，并被认为是值得通过基金会的知识分子优点和更广泛的影响审查标准通过评估来支持的。

项目成果

MicroLED/LED electro-optical integration techniques for non-display applications

• DOI: 10.1063/5.0125103
• 发表时间: 2023-06-01
• 期刊: APPLIED PHYSICS REVIEWS
• 影响因子: 15
• 作者: Kumar，V.;Kymissis，I.
• 通讯作者: Kymissis，I.

44‐4: A Brief Survey of MicroLED Technologies

44–4：MicroLED 技术简介

• DOI: 10.1002/sdtp.13951
• 发表时间: 2020
• 期刊: SID Symposium Digest of Technical Papers
• 作者: Kymissis, Ioannis;Behrman, Keith
• 通讯作者: Behrman, Keith

MicroLED light source for optical sectioning structured illumination microscopy

用于光学切片结构照明显微镜的 MicroLED 光源

• DOI: 10.1364/oe.486754
• 发表时间: 2023
• 期刊: Optics Express
• 影响因子: 3.8
• 作者: Kumar, Vikrant;Behrman, Keith;Speed, Forest;Saladrigas, Catherine A.;Supekar, Omkar;Huang, Zicong;Bright, Victor M.;Welle, Cristin G.;Restrepo, Diego;Gopinath, Juliet T.
• 通讯作者: Gopinath, Juliet T.

Enhanced microLED efficiency via strategic pGaN contact geometries

• DOI: 10.1364/oe.425800
• 发表时间: 2021-05-10
• 期刊: OPTICS EXPRESS
• 影响因子: 3.8
• 作者: Behrman, Keith;Kymissis, Ioannis
• 通讯作者: Kymissis, Ioannis

Ultra‐Thin Ceramic Substrates for Improved Heat sinking for MicroLEDs

• DOI: 10.1002/admt.202300390
• 发表时间: 2023-08
• 期刊: Advanced Materials Technologies
• 影响因子: 6.8
• 作者: Christine K. McGinn;Vikrant Kumar;Megan Noga;Zachary A. Lamport;Ioannis Kymissis