Collaborative Research: MRI Consortium: Development of Fiber-Coupled Stimulated Emission Depletion Microscopy (STED)

合作研究：MRI 联盟：光纤耦合受激发射损耗显微镜 (STED) 的开发

• 批准号: 1919361
• 负责人: Emily Gibson
• 金额: $ 40.12万
• 依托单位: University of Colorado at Denver-Downtown Campus
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-10-01 至 2024-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1919361&HistoricalAwards=false
• 关键词: Collaborative; Research; MRI; Consortium; Development

An award is made to the University of Colorado Boulder, University of Colorado Anschutz Medical Campus and the University of Denver to develop a unique miniature optical fiber-coupled microscope that will enable flexible imaging at unprecedented spatial resolution. Developed with a diverse team that includes engineers, physicists, and neuroscientists, this cutting-edge microscope will not only provide new fundamental and practical insights across research areas, but will also yield far-reaching impacts through intellectual property development and future commercialization and deployment in industry. The research team will use the new instrument to expand ongoing efforts to mentor and inspire young students from groups underrepresented in Science Technology Engineering and Mathematics (STEM) fields.Real-time in-brain imaging has made tremendous advances in the past few years, allowing unprecedented inquiry into how dynamic structures such as dendritic spines enable brain functions such as learning, memory, cognition, and behavior. However, future advances in observation-based understanding of the function and response of these cellular structures are limited by the blur inherent to light's fundamental properties that obscures these features. Based on the established and Nobel Prize-winning principle of Stimulated Emission Depletion (STED) microscopy, the instrument developed in this collaborative project will be capable of a 5X increase in spatial resolution. This miniature microscope will enable, for the first time, imaging of small structures such as synaptic spine dynamics and changes in myelination underlying behaviors and social interactions in behaving animals. It will also enable lithography in 3D materials at unprecedented spatial scale aimed towards the next generation of electronic devices.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

向科罗拉多大学博尔德大学，科罗拉多大学安索斯大学医学校园和丹佛大学颁发了奖项，以开发独特的微型光纤耦合显微镜，该显微镜将以前所未有的空间分辨率启用灵活的成像。该尖端显微镜由包括工程师，物理学家和神经科学家在内的多元化团队开发，不仅将在研究领域提供新的基本和实用见解，而且还将通过知识产权发展以及未来的商业化和行业部署产生深远的影响。研究团队将使用新工具扩大持续的努力，从而指导和激发科学技术工程和数学领域不足的年轻学生（STEM）领域。真实的时间内部成像在过去几年中取得了巨大的进步，从而使人们对诸如DENDRITIC SPINE SPINE SEPINE SERMITITION诸如启用脑脑功能的动态结构进行了良好的询问，并启用了诸如学习脑功能，并进行了记忆，诸如学习，并进行了记忆。但是，基于观察的对这些细胞结构的功能和响应的理解的进展受到掩盖这些特征的基本特性所固有的模糊限制。根据刺激排放耗尽（STED）显微镜的既定和诺贝尔奖原则，该协作项目中开发的仪器将能够提高空间分辨率5倍。这种微型显微镜将首次实现小结构的成像，例如突触脊柱动力学以及髓鞘形成行为的基础行为和社交相互作用的变化。它还将以前所未有的空间规模在3D材料中启用光刻，旨在针对下一代电子设备。该奖项反映了NSF的法定任务，并被认为是值得通过基金会的知识分子和更广泛影响的评估评估来审查标准的评估值得支持的。

项目成果

Comparison of Coherent and Incoherent Donut Beams for Deep Tissue STED Microscopy

• DOI: 10.1364/cleo_si.2023.sm1i.2
• 发表时间: 2023-05
• 期刊: 2023 Conference on Lasers and Electro-Optics (CLEO)
• 作者: Thariq Shanavas;R. McLeod;M. Siemens;J. Gopinath

OpenSTED: Inexpensive and open-source implementation of Dynamic Intensity Minimum (DyMIN) for Stimulated Emission Depletion (STED) microscopy

OpenSTED：用于受激发射损耗 (STED) 显微镜的动态强度最小值 (DyMIN) 的廉价开源实施

• DOI: 10.1364/ntm.2021.nw4c.3
• 发表时间: 2021
• 期刊: Biophotonics Congress 2021
• 作者: Meyer, Stephanie A.;Siemens, Mark E.;Gopinath, Juliet T.;Restrepo, Diego;Gibson, Emily A.
• 通讯作者: Gibson, Emily A.

Two-photon, fiber-coupled, super-resolution microscope for biological imaging

• DOI: 10.1063/5.0075012
• 发表时间: 2022-03-01
• 期刊: APL PHOTONICS
• 影响因子: 5.6
• 作者: Heffernan，Brendan M.;Riley，Peter S.;Gopinath，Juliet T.
• 通讯作者: Gopinath，Juliet T.

Two-photon Fiber STED Microscope Using Polarization Maintaining Fiber

使用保偏光纤的双光子光纤 STED 显微镜

• DOI: 10.1364/cleo_si.2021.sw2d.2
• 发表时间: 2021
• 期刊: Conference on Lasers and Electro-Optics
• 作者: Heffernan, Brendan M.;Riley, Peter;Supekar, Omkar D.;Meyer, Stephanie A.;George, Nicholas M.;Restrepo, Diego;Siemens, Mark E.;Gibson, Emily A.;Gopinath, Juliet T.
• 通讯作者: Gopinath, Juliet T.