MRI Development of a fast P3D-STED Microscope

快速 P3D-STED 显微镜的 MRI 开发

• 批准号: 1337573
• 负责人: Emily Gibson
• 金额: $ 105万
• 依托单位: University of Colorado at Denver-Downtown Campus
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-09-01 至 2019-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1337573&HistoricalAwards=false
• 关键词: MRI; Development; fast; P3D; STED

Title: MRI Development of a FAST P3D-STED MicroscopeAn NSF MRI Development Grant award is made to a collaborative team of physicists, engineers, and neuroscientists at the University of Colorado Denver to further develop the capabilities of Stimulated Emission Depletion (STED) microscopy. The goal of the project is to develop a FAST P3D-STED microscope (fast acquisition, photoactivation, 3-dimensional enhanced resolution STED microscope). Historically, light microscopy has been limited in its resolution by the Diffraction Barrier - a fundamental physical limit due to the property of photons of spreading out when they pass by an edge, such as the aperture in a microscope. In the past decade, several methods have emerged that can "break" the diffraction barrier, causing a revolution in the field of biological imaging and opening a major window of opportunity for studies that heretofore have been impossible. STimulated Emission Depletion (STED) microscopy, invented by Stefan Hell, is one such super-resolution technique, allowing fluorescence imaging at resolutions of tens of nanometers. With continuing improvements in the technology, STED is now able to be utilized with a wide range of fluorescent dyes and genetically encoded fluorophores and has advantages for fast, live cell, and in vivo imaging. The investigators will further push the current capabilities of STED microscopy by developing a new STED microscope to allow high speed, sub-diffraction limit imaging combined with photoactivation capabilities. This microscope will allow researchers to not only observe small objects with sub-diffraction resolution, but also enable control of dynamic processes and observation in real time.The projects enabled by the instrument include studies of the dynamic organization of protein complexes in synapses upon control of neural plasticity, studies of the molecular-level mechanisms of odor transduction by direct stimulation of the transduction pathway, and methods for writing/reading bits at sub-diffraction dimensions in high density data storage materials. The potential societal impacts of this research include improved treatment of neurological disorders and advances in information handling and storage. The FAST P3D STED microscope will be housed in the Advanced Light Microscopy Core (ALMC) facility at the University of Colorado Denver, a successful shared user facility that has personnel and resources to train new users and operate and maintain the instrument. Researchers, postdoctoral trainees, and students from a variety of science and engineering departments at the University of Colorado Denver, University of Colorado Boulder and other universities will be able to utilize the microscope for their research. Outreach to new users will be accomplished through seminars put on by the ALMC and research presentations at scientific meetings. The investigators are particularly committed to promoting student training and advancing participation of under-represented minorities in science as evidenced by their involvement in the Building Research Achievement in Neuroscience (BRAiN) program, a summer program designed to help meet the challenge to reduce the Neuroscience research participation gap by preparing diverse undergraduates in the Rocky Mountain and Southwest Region for successful entry to Neuroscience Ph.D. programs. The capability of the STED microscope to see the inner workings of cells at unprecedented resolution captures the imagination of these future scientists.

标题：在科罗拉多大学丹佛大学的物理学家，工程师和神经科学家的合作团队中，MRI开发的快速P3D MIRSF MRI Development Grant奖是为了进一步发展刺激排放耗竭（STED）显微镜的能力的合作团队。该项目的目的是开发快速的P3D缝显微镜（快速获取，光激活，3维增强的分辨率Stated显微镜）。 从历史上看，光学显微镜在衍射屏障的分辨率上受到限制 - 由于光子通过边缘经过时（例如显微镜中的孔径）时，它们的基本物理极限。在过去的十年中，已经出现了几种可以“打破”衍射障碍的方法，从而在生物成像领域引起了革命，并为迄今为止不可能的研究开辟了一个主要的研究窗口。由Stefan Hell发明的刺激发射耗竭（Sted）显微镜就是这样一种超分辨率技术，可以在数十纳米的分辨率下进行荧光成像。随着技术的持续改进，Sted现在可以使用多种荧光染料和遗传编码的荧光团使用，并且具有快速，活细胞和体内成像的优点。研究人员将通过开发新的Sted显微镜来进一步推动STED显微镜的当前功能，从而允许高速，亚分曲极限成像与光激活能力相结合。 This microscope will allow researchers to not only observe small objects with sub-diffraction resolution, but also enable control of dynamic processes and observation in real time.The projects enabled by the instrument include studies of the dynamic organization of protein complexes in synapses upon control of neural plasticity, studies of the molecular-level mechanisms of odor transduction by direct stimulation of the transduction pathway, and methods for writing/reading bits at sub-diffraction高密度数据存储材料的尺寸。这项研究的潜在社会影响包括改善神经系统疾病的治疗以及信息处理和存储方面的进步。快速的P3D Sted显微镜将安置在科罗拉多大学丹佛大学的高级光学显微镜核心（ALMC）设施中，这是一个成功的共享用户设施，拥有人员和资源来培训新用户并运行和维护乐器。 科罗拉多大学丹佛分校，科罗拉多大学博尔德大学和其他大学的研究人员，博士后学员以及来自各种科学和工程系的学生将能够利用显微镜进行研究。将通过ALMC和科学会议上的研究演讲来实现与新用户的宣传。 调查人员特别致力于促进学生培训并推进代表性不足的科学参与，这是由于他们参与建筑研究成就神经科学（Brain）计划（Brain）计划，这是一项夏季计划，这是一个夏季计划，旨在帮助挑战，以减少神经科学研究的参与差距，从而通过在Rocky Mountain和Southwest Southwest Neur的范围内培养Neur phorectience neurouce neurouce neurouce neurouce neurouce的差异不足。程序。 在前所未有的分辨率上看到细胞内部功能的st型显微镜的能力捕捉了这些未来科学家的想象。