Fluorescent Probes for the Detection of Ethylene

用于检测乙烯的荧光探针

• 批准号: 1900482
• 负责人: Brian Michel
• 金额: $ 30万
• 依托单位: University of Denver
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-07-01 至 2023-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1900482&HistoricalAwards=false
• 关键词: Fluorescent; Probes; Detection; Ethylene

Ethylene is an important chemicals in modern society, with impacts ranging from the production of plastics and other chemicals to playing a significant role in the food supply chain as the primary ripening hormone of some fruit. As a result, there has been a significant effort to detect this small molecule via instrumentation and with molecular recognition. While these efforts have led to some sensitive approaches for ethylene detection, methods for detecting ethylene in complex environments (such as inside a plant cell) do not currently exist. In this project, Professor Michel of the University of Denver is developing new small molecule probes that increase fluorescence upon reaction with ethylene. An understanding of the relationship between the structure of the probe and its signaling property in a specific environment are critical to the sensitive detection of ethylene. Fluorescent and color-producing probes have the unique ability to produce color within otherwise invisible chemical processes. This research is being developed into an integrated lecture-lab module for undergraduate organic students to provide context to underrepresented topics. Professor Michel develops outreach demonstrations at local high schools. Due to the desire to understand and control ethylene in the fruit supply chain, this research may have a broader impact on society by increasing the understanding of the molecular roles of ethylene in the ripening processes; thereby preventing premature food spoilage and reducing agricultural waste. Further, the graduate and undergraduate students working on this project are underrepresented minorities who are gaining a unique skill set due to the interdisciplinary nature of this project. With funding from the Chemical Structure, Dynamics, and Mechanisms B program of the Chemistry Division, Professor Michel of the University of Denver is developing new small molecule fluorescent probes for the detection of ethylene. Due to the relatively small and unreactive nature of ethylene under the confined conditions of living systems, an organometallic-based approach is being developed, which requires a fundamental understanding of the relationship between the ligands bound to the metal center and stoichiometric reactivity with ethylene. This research determines the optimal probes for a particular application, such as the detection of ethylene in relevant biological systems including A. thaliana and E. coli. Detection of ethylene in these organisms is being investigated with probe modifications tailored to the specific goals and challenges presented by these systems. The detection of gaseous ethylene in the atmosphere including from ripening fruit is also being investigated.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

乙烯是现代社会中的重要化学物质，其影响范围从塑料和其他化学物质的产生到在食品供应链中起重要作用，这是某些水果的主要成熟激素。结果，已经付出了很大的努力来通过仪器和分子识别来检测这一小分子。尽管这些努力导致了某些对乙烯检测的敏感方法，但目前尚不存在在复杂环境中检测乙烯（例如在植物细胞内）中检测乙烯的方法。在这个项目中，丹佛大学的米歇尔教授正在开发新的小分子探针，这些探针会在与乙烯反应时增加荧光。在特定环境中对探针结构及其信号传导特性之间的关系的理解对于敏感的乙烯检测至关重要。荧光和产生颜色的探针具有独特的能力，可以在原本看不见的化学过程中产生颜色。这项研究已发展为一个综合的演讲模块，供本科有机学生为代表性不足的主题提供背景。米歇尔教授在当地高中开展了外展示威。由于希望在水果供应链中理解和控制乙烯，这项研究可能会通过增加对乙烯在成熟过程中的分子作用的理解来对社会产生更大的影响；从而防止过早食物变质并减少农业废物。此外，从事该项目的研究生和本科生的人数不足，他们由于该项目的跨学科性质而获得了独特的技能。通过化学结构，动力学和机制B计划的资金，丹佛大学的米歇尔教授正在开发新的小分子荧光探针以检测乙烯。由于在生命系统的狭窄条件下，乙烯的相对较小且不反应性的性质，正在开发一种基于有机金属的方法，这需要对与金属中心结合的配体之间的关系以及与乙烯的化学反应性有基本的理解。这项研究确定了特定应用的最佳探针，例如在包括A. thaliana和E. coli在内的相关生物系统中检测乙烯。正在研究这些生物体中乙烯的检测，并根据这些系统提出的特定目标和挑战量身定制的探针修改。还正在研究大气中的气态乙烯，包括成熟的果实。该奖项反映了NSF的法定任务，并被认为是值得通过基金会的知识分子优点和更广泛影响的评估标准通过评估来支持的。

项目成果

Steric modulation of CAACs controls orientation and ethenolysis performance

CAAC 的空间调节控制方向和乙烯醇分解性能

• DOI: 10.1016/j.checat.2023.100764
• 发表时间: 2023
• 期刊: Chem Catalysis
• 作者: Jensen, Katrina H.;Michel, Brian W.
• 通讯作者: Michel, Brian W.

Detection of Ethylene with Defined Metal Complexes: Strategies and Recent Advances

• DOI: 10.1002/anse.202200058
• 发表时间: 2023-03-01
• 期刊: ANALYSIS & SENSING
• 作者: Jensen，Katrina H.;Michel，Brian W.
• 通讯作者: Michel，Brian W.

Ligand-Directed Approach to Activity-Based Sensing: Developing Palladacycle Fluorescent Probes That Enable Endogenous Carbon Monoxide Detection

• DOI: 10.1021/jacs.0c06405
• 发表时间: 2020-09-16
• 期刊: JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
• 影响因子: 15
• 作者: Morstein, Johannes;Hofler, Denis;Chang, Christopher J.
• 通讯作者: Chang, Christopher J.