Core 3: Chemical Biology & Materials Tools (CBMT)

核心 3：化学生物学

• 批准号: 10626287
• 负责人: David Michael Chenoweth
• 金额: $ 14.23万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-05-08 至 2028-04-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10626287
• 关键词: Agonist; Award; Biological; Biological Process; Biology; CD47 gene; Cell physiology; Cells; Chemicals; Chromosomal Instability; Collaborations; Collagen; Complex; Detection; Development; Dimerization; Environment; Event; Fluorescent Probes; Genomic Instability; Gibberellins; Glean; Goals; Hybrids; Image; Individual; Laboratories; Macrophage; Meiosis; Membrane; Mitosis; Molecular; Monitor; Natural Products; Nucleic Acids; Optics; Pathway interactions; Peptides; Process; Proteins; Reagent; Reporting; Research; Research Project Grants; Resources; Sirolimus; Structure; System; Technology; Time; Variant; Whole Organism; Work; antagonist; anti-tumor immune response; biological systems; cancer cell; design; genetic approach; genetic regulatory protein; genome integrity; innovation; insight; live cell imaging; novel strategies; nucleic acid structure; optogenetics; peptidomimetics; programs; protein aggregation; sensor; small molecule; synergism; tool; trafficking

Project Summary Chemical Biology tools for probing, manipulating, and imaging biological systems can provide deep insights into fundamental processes. Chemical dimerization and protein localization strategies, fluorescent probes and sensors for analyte detection, and small molecules for perturbing biological pathways and process have become important research tools for gleaning deeper insight into biological processes. The development of optically triggered variants of these important chemical biology tools has provided new and more powerful approaches to probing, manipulating, and imaging key pathways and processes with new levels of spatial and temporal control not previously available. Despite the utility of molecular chemical biology approaches, many of the tools and reagents are not readily available due to the need for on-demand synthesis and molecular customization depending on the pathways and processes being studied and the lack of commercially availability. The proposed Chemical Biology and Materials Core will solve these problems by working directly with the individual projects of the program to design, synthesize, and implement cutting edge Chemical biology tools customized to the project research aims. The Chemical Biology Core will also serve as a resource throughout the award period to develop new approaches and implement key technologies in synergy with the MAC core and individual projects. In Aim 1 the core will develop chemical dimerizers for optogenetic control of key biomolecule localization and activation events. In Aim 2 the core will develop peptide based chemical probes for interrogation of key biological pathways and processes.

项目摘要 化学生物学工具用于探测，操纵和成像生物系统可以提供 深入了解基本过程。化学二聚化和蛋白质定位 用于分析物检测的策略，荧光探针和传感器以及小分子 扰动生物途径和过程已成为收集的重要研究工具 对生物过程的深入了解。这些光学触发变体的开发 重要的化学生物学工具为探测提供了新的，更强大的方法， 操纵新级别的空间和时间级别的关键途径和过程 控制以前无法使用。尽管分子化学生物学方法有用，但 由于需要按需合成，许多工具和试剂不容易获得 以及分子定制，具体取决于所研究的途径和过程 缺乏商业上的可用性。拟议的化学生物学和材料核心将解决 这些问题直接与计划的各个项目一起设计， 合成并实施为项目定制的尖端化学生物学工具 研究目的。化学生物学核心还将作为整个奖项的资源 开发新方法并与MAC核心协同实施关键技术的时期 和个别项目。在AIM 1中，核心将开发用于光遗传控制的化学二聚体 关键的生物分子定位和激活事件。在AIM 2中，核心将发展基于肽的 化学探针询问关键的生物途径和过程。