Natural sources and microbial transformation of marine halogenated pollutants

海洋卤化污染物的自然来源和微生物转化

基本信息

批准号：
1837116
负责人：
Bradley Moore
金额：
$ 76.73万
依托单位：
University of California-San Diego Scripps Inst of Oceanography
依托单位国家：
美国
项目类别：
Continuing Grant
财政年份：
2018
资助国家：
美国
起止时间：
2018-09-15 至 2024-08-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=1837116&HistoricalAwards=false
关键词：
Natural sources microbial transformation marine

项目摘要

Natural polybrominated organic compounds such as hydroxylated polybrominated diphenyl ethers (OH-BDEs) and polybrominated pyrroles (PBPs) have recently emerged as chemicals of human health concern. These natural product relatives of man-made halogenated persistent organic pollutants (POPs) are widely distributed throughout the marine food web and accumulate in seafood sources consumed by humans. Researchers have demonstrated that OH-BDEs such as 6-OH-BDE-47 (thyroid hormone receptor) and PBPs such as tetrabromopyrrole (ryanodine receptor) are potent toxins and thus pose a potential risk to humans. Many fundamental questions however remain about the extent of sources for these natural organobromine molecules, how these chemicals enter and move through the marine food web, whether changes in the climate will impact their production and accumulation, and whether humans are more or less impacted by natural halogenated POPs versus their man-made counterparts. This project seeks to advance the biology and chemistry of marine contaminants of emerging concern. A better understanding of the sources and sinks of these compounds will improve public understanding about links between oceans and human health and help improve guidelines and evidence-based policies regarding fish consumption and health. The project will support a graduate student and a postdoctoral researcher. The project is jointly supported by NSF and by the National Institute for Environmental Health Sciences (NIEHS).Recent discoveries by these investigators have rigorously established the genetic and biochemical basis for the microbial synthesis of natural OH-BDE molecules in diverse lineages of marine bacteria. However, the global distribution and ubiquity of these polybrominated POPs in marine biota cannot be fully explained by the sources discovered thus far, suggesting additional biogenic sources exist and are actively contributing to OH-BDE and MeO-BDE accumulation in the marine food web. This information is critical to more accurately identify trophic connections and interconversions that lead to natural PBDE accumulation in marine fish and ultimately, human dietary exposure risks. In this project, new genetic and biochemical evidence for the biosynthesis and biotransformation of PBDE molecules will be established for marine macroalgae, a conspicuous but uncharacterized source of PBDE molecules in marine habitats, using transcriptome analysis coupled with biochemical enzyme characterization. Additional microbial sources for PBDE synthesis/transformation will be characterized by the comprehensive analysis of fish and marine-mammal associated microbiomes using integrated genomic and metabolomic approaches combined with experimental microbiome enrichment reactors amended with PBDE molecules or biosynthetic substrates.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

天然多溴有机化合物，例如羟基化多溴二苯醚（OH-BDE）和多溴吡咯（PBP）最近已成为关注人类健康的化学品。这些人造卤化持久性有机污染物 (POP) 的天然产物广泛分布在整个海洋食物网中，并在人类消费的海鲜来源中积累。研究人员已经证明，6-OH-BDE-47（甲状腺激素受体）等 OH-BDE 和四溴吡咯（兰尼碱受体）等 PBP 是强效毒素，因此对人类构成潜在风险。然而，许多基本问题仍然存在，包括这些天然有机溴分子的来源范围、这些化学物质如何进入并通过海洋食物网、气候变化是否会影响其生产和积累，以及人类是否或多或少受到自然环境的影响。卤化持久性有机污染物与人造持久性有机污染物的比较。该项目旨在推进新兴关注的海洋污染物的生物学和化学研究。更好地了解这些化合物的来源和汇将提高公众对海洋与人类健康之间联系的理解，并有助于改进有关鱼类消费和健康的指南和循证政策。该项目将支持一名研究生和一名博士后研究员。该项目由 NSF 和国家环境健康科学研究所 (NIEHS) 联合支持。这些研究人员最近的发现为不同海洋细菌谱系中天然 OH-BDE 分子的微生物合成建立了严格的遗传和生化基础。然而，这些多溴化持久性有机污染物在海洋生物群中的全球分布和普遍性无法用迄今为止发现的来源来完全解释，这表明存在其他生物来源，并且正在积极促进海洋食物网中 OH-BDE 和 MeO-BDE 的积累。这些信息对于更准确地识别导致海洋鱼类自然积累 PBDE 并最终导致人类饮食暴露风险的营养联系和相互转化至关重要。在该项目中，将利用转录组分析与生化酶表征相结合，为海洋大型藻类（海洋生境中常见但未表征的 PBDE 分子来源）建立 PBDE 分子生物合成和生物转化的新遗传和生化证据。用于 PBDE 合成/转化的其他微生物来源的特点是，使用综合基因组学和代谢组学方法，结合用 PBDE 分子或生物合成底物修正的实验微生物组富集反应器，对鱼类和海洋哺乳动物相关微生物组进行综合分析。该奖项反映了 NSF 的法定使命和通过使用基金会的智力优点和更广泛的影响审查标准进行评估，该项目被认为值得支持。

项目成果

期刊论文数量（13）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Temporal, Environmental, and Biological Drivers of the Mucosal Microbiome in a Wild Marine Fish, Scomber japonicus

DOI：
10.1128/msphere.00401-20
发表时间：
2019-08
期刊：
mSphere
影响因子：
4.8
作者：
J. Minich;Semar Petrus;J. Michael;T. Michael;R. Knight;E. Allen
通讯作者：
J. Minich;Semar Petrus;J. Michael;T. Michael;R. Knight;E. Allen

A genomic view of trophic and metabolic diversity in clade-specific Lamellodysidea sponge microbiomes

DOI：
10.1186/s40168-020-00877-y
发表时间：
2020-06-23
期刊：
MICROBIOME
影响因子：
15.5
作者：
Podell, Sheila;Blanton, Jessica M.;Allen, Eric E.
通讯作者：
Allen, Eric E.

Host biology, ecology and the environment influence microbial biomass and diversity in 101 marine fish species.

DOI：
10.1038/s41467-022-34557-2
发表时间：
2022-11-17
期刊：
NATURE COMMUNICATIONS
影响因子：
16.6
作者：
Minich, Jeremiah J.;Harer, Andreas;Vechinski, Joseph;Frable, Benjamin W.;Skelton, Zachary R.;Kunselman, Emily;Shane, Michael A.;Perry, Daniela S.;Gonzalez, Antonio;McDonald, Daniel;Knight, Rob;Michael, Todd P.;Allen, Eric E.
通讯作者：
Allen, Eric E.

The Southern Bluefin Tuna Mucosal Microbiome Is Influenced by Husbandry Method, Net Pen Location, and Anti-parasite Treatment

南方蓝鳍金枪鱼粘膜微生物群受到饲养方法、网栏位置和抗寄生虫处理的影响

DOI：
10.3389/fmicb.2020.02015
发表时间：
2020
期刊：
Frontiers in Microbiology
影响因子：
5.2
作者：
Minich, Jeremiah J.;Power, Cecilia;Melanson, Michaela;Knight, Rob;Webber, Claire;Rough, Kirsten;Bott, Nathan J.;Nowak, Barbara;Allen, Eric E.
通讯作者：
Allen, Eric E.

Impacts of the Marine Hatchery Built Environment, Water and Feed on Mucosal Microbiome Colonization Across Ontogeny in Yellowtail Kingfish, Seriola lalandi