Collaborative Research: SG: RUI: Exploring the genetic basis of phenotypic novelty in experimental hybrids of monkeyflowers (Mimulus)

合作研究：SG：RUI：探索猴花（Mimulus）实验杂交种表型新颖性的遗传基础

基本信息

批准号：
1754075
负责人：
Arielle Cooley
金额：
$ 7.26万
依托单位：
Whitman College
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
2018
资助国家：
美国
起止时间：
2018-05-01 至 2022-08-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=1754075&HistoricalAwards=false
关键词：
Collaborative Research SG RUI Exploring

项目摘要

Life on earth exhibits a dazzling array of patterns. These range from internal patterns, like the repetition of vertebrae in the spinal cord, to external patterns like the stripes of black pigment on a zebra. Pigmentation has become a powerful model for exploring the origins and genetic mechanisms behind these types of patterns. For example, new information about insect development has been learned by studying the colorful eyespots in butterflies. In plants, the mechanisms of pigment production have been well-studied, but surprisingly little is known about the mechanisms of complex patterns of pigmentation. Filling this knowledge gap is the goal of this research. This project will study two species of monkeyflower; each has flowers with strikingly different color patterns. What's more, when the two species are mated together, new intricate and complex color patterns emerge. Since these new pigmentation patterns are seen only when different species are mated together, it suggests that interactions between their genomes give rise to the new patterns. Exploring how such interactions between different genomes can produce unexpected new traits is a primary focus of this research. One master's student, six undergraduates, and six high school students will be recruited to carry out this work. These students will be exposed to high-impact learning opportunities as they are immersed in hands-on scientific research. At least two of the high school students will be selected from a high school that specifically serves disadvantaged youth.The pigmentation patterns in the hybrids of Mimulus cupreus x M. luteus var. variegatus are too intricate to quantify using standard approaches. Instead, a new digital image analysis pipeline is being developed. This approach will be useful for analyzing spatial patterning in monkeyflowers and in other taxa. The researchers will use this new method to identify and quantify elements of floral color pattern in a phenotypically diverse F2 hybrid population. Next-generation sequencing will be used to map the genomic regions responsible for the observed variation in flower patterns. Because M. cupreus and M. l. variegatus also differ in yellow carotenoid pigmentation, the same phenotypic and genetic datasets will be used to identify the genetic basis of divergent carotenoid production, which is an important but under-studied component of plant physiology.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

地球上的生活表现出令人眼花and乱的图案。 These range from internal patterns, like the repetition of vertebrae in the spinal cord, to external patterns like the stripes of black pigment on a zebra.色素沉着已成为探索这些类型模式背后的起源和遗传机制的强大模型。例如，通过研究蝴蝶中的五颜六色眼点了解了有关昆虫发育的新信息。 In plants, the mechanisms of pigment production have been well-studied, but surprisingly little is known about the mechanisms of complex patterns of pigmentation.填补这一知识差距是这项研究的目标。该项目将研究两种猴子。每种花都有不同的颜色图案。更重要的是，当两个物种交配在一起时，出现了新的复杂且复杂的颜色模式。 Since these new pigmentation patterns are seen only when different species are mated together, it suggests that interactions between their genomes give rise to the new patterns.探索不同基因组之间的这种相互作用如何产生意外的新特征是这项研究的主要重点。一名硕士学生，六名本科生和六名高中生将被招募来进行这项工作。这些学生将沉浸在动手科学研究中，以接触高影响力的学习机会。 At least two of the high school students will be selected from a high school that specifically serves disadvantaged youth.The pigmentation patterns in the hybrids of Mimulus cupreus x M. luteus var. Variegatus过于复杂，无法使用标准方法进行量化。取而代之的是，正在开发新的数字图像分析管道。这种方法将有助于分析猴子花和其他分类单元中的空间图案。 The researchers will use this new method to identify and quantify elements of floral color pattern in a phenotypically diverse F2 hybrid population.下一代测序将用于绘制负责观察到的花模式变化的基因组区域。因为M. Cupreus和M. L。 variegatus also differ in yellow carotenoid pigmentation, the same phenotypic and genetic datasets will be used to identify the genetic basis of divergent carotenoid production, which is an important but under-studied component of plant physiology.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.