MRI: Acquisition of an X-band Electron Paramagnetic Resonance (EPR) Spectrometer to Enhance the Infrastructure for Research in Redox Processes in Chemistry and Biology

MRI：购买 X 波段电子顺磁共振 (EPR) 波谱仪，以增强化学和生物学氧化还原过程研究的基础设施

基本信息

批准号：
1726244
负责人：
Alison Fout
金额：
$ 27.43万
依托单位：
University of Illinois at Urbana-Champaign
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
2017
资助国家：
美国
起止时间：
2017-08-15 至 2020-07-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=1726244&HistoricalAwards=false
关键词：
MRI Acquisition band Electron Paramagnetic

项目摘要

This award is supported by the Major Research Instrumentation (MRI) and the Chemistry Research Instrumentation(CRIF) Programs. Professor Alison Fout from University of Illinois Urbana-Champaign (UIUC) and colleagues Robert Gennis, Yi Lu and Dean Olson have acquired an X-band electron paramagnetic resonance (EPR) spectrometer. This instrument allows research in a variety of fields such as those that provide insight on the behavior of biologically-relevant and environmentally-important species. In general, an EPR spectrometer yields detailed information on the geometric and electronic structures of molecular and solid state materials. It may also be used to obtain information about the lifetimes of short-lived, highly reactive species involved in valuable chemical transformations as well as the initiation of pathological tumor growth. These studies impact several areas, from the synthesis of inorganic and organic molecules to the development of new solid state materials to compounds of magnetic and biological and medical interest. The instrument serves to train the next generation of student researchers both at UIUC and the nearby institution, Eastern Illinois University.This EPR spectrometer enhances research and education at all levels. It may impact studies linking proton pumping to hydride transfer by studying the mechanism of transhydrogenase, tuning redox potentials of metal centers in a single protein and developing cobalt(I) catalysts featuring strong-field ligands for Carbon-X bond formation. The instrumentation is also used in the studies of compounds prepared by bio-inspired complexes for oxyanion reduction. Electron transfer in the bc1 complex and investigations of the five catalytic sites of ubisemiquinones are also under consideration. The spectrometer benefits investigations of redox enzymes, advances chemical vapor deposition (CVD), and explores oxidative stress in ROS-sensitive enzymes. In addition, the EPR is used for studies tuning redox potentials of metal centers in a single protein and characterizing intermediate states in P450 catalysis.

该奖项得到了主要的研究工具（MRI）和化学研究工具（CRIF）计划的支持。伊利诺伊大学Urbana-Champaign（UIUC）的Alison Fout教授和Robert Gennis，Yi Lu和Dean Olson的同事获得了X波段电子顺磁共振（EPR）光谱仪。该仪器允许在各种领域进行研究，例如提供有关生物学上与生物学和环境重要物种的行为的洞察力的研究。通常，EPR光谱仪得出有关分子和固态材料的几何和电子结构的详细信息。它还可以用来获取有关参与有价值的化学转化以及病理肿瘤生长的短寿命，高反应性物种的寿命的信息。这些研究影响了几个领域，从无机和有机分子的合成到开发新的固态材料到磁性和生物学兴趣的化合物。该工具旨在培训UIUC和附近伊利诺伊州东部机构的下一代学生研究人员。这一EPR光谱仪可增强各级研究和教育。它可能会通过研究经氢化酶的机制，单个蛋白质中金属中心的氧化还原电位并发展出具有碳-X键形成的强场配体的催化剂（i）催化剂，从而影响将质子泵送与氢化物转移联系起来的研究。该仪器还用于通过生物启发的复合物制备的化合物进行氧气还原。在BC1复合物中的电子转移以及对泛丝酮五个催化位点的研究也在考虑。光谱仪受益于氧化还原酶，化学蒸气沉积（CVD）的研究，并探索ROS敏感酶中的氧化应激。此外，EPR用于研究单个蛋白质中金属中心的氧化还原电位的研究，并在P450催化中表征了中间状态。