SusChEM: GOALI: Harnessing the Antimicrobial Properties of Copper to Control Legionella in Plumbing Systems

SusChEM：目标：利用铜的抗菌特性来控制管道系统中的军团菌

• 批准号: 1706733
• 负责人: Marc Edwards
• 金额: $ 37.98万
• 依托单位: Virginia Polytechnic Institute and State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-07-01 至 2021-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1706733&HistoricalAwards=false
• 关键词: SusChEM; GOALI; Harnessing; Antimicrobial; Properties

Proposal Number: 1706733PI Name: Marc A. Edwards The growing public health threat in drinking water from opportunistic pathogens (OPs), such as Legionella, calls for practical engineering solutions that are compatible with sustainability goals. Taking advantage of the natural disinfecting properties of copper (Cu) pipes, which are widely used in plumbing systems, is an attractive approach for control of Legionella; however, Cu is not consistently effective in practice, as it sometimes inhibits and other times stimulates Legionella growth. This research seeks to address this fundamental knowledge gap through bench, pilot, and field-scale studies to identify the water chemistries that enhance or inhibit Cu-driven disinfection of Legionella. This research is potentially transformative as it will inform practical, economical, and sustainable means of addressing a key public health threat just as trillions of dollars will be invested to upgrade antiquated water infrastructure.The overarching hypothesis for this research is that water chemistry directly and indirectly influences Cu toxicity towards Legionella. The specific objectives are to: 1) Determine the effects of Cu speciation, complexation, and precipitation in governing toxicity towards Legionella under conditions relevant to hot water building plumbing systems; 2) Explore the role of microbial communities inhabiting plumbing systems to mediate toxicity of Cu, e.g., through shielding or responding to nutrients released from corrosion reactions (e.g., H2 evolved), and confirm Objective 1 phenomena under realistic pilot-scale plumbing conditions; and 3) Conduct a field survey to validate relationships among physicochemical, Cu speciation/solubility, and microbial factors in real-world plumbing systems and illuminate drivers of Cu-enhanced versus Cu-inhibited Legionella growth. This study will be the first systematic examination of the speciation- and solubility-dependent nature of Cu toxicity towards Legionella in potable water systems, particularly within hot water that is most relevant to OP proliferation. Of particular interest is the occurrence and behavior of cuprous (Cu1+) ions. Cu1+ is probably non-toxic to Legionella and may even indirectly stimulate its growth by catalyzing deposition corrosion of less noble metal plumbing materials which will then provide H2 for autotrophic hydrogen-oxidizing bacteria. Cupric (Cu2+) ions have strong biocidal properties, but effectiveness may be limited by temperature, redox, inorganic and organic ligands, corrosion control agents, and biofilm properties. An innovative combination of tools will be used to characterize complex interdependencies among the microbial community composition, physicochemical properties, Cu speciation, and Legionella, including next-generation DNA sequencing, green-fluorescence protein modified strains, flow cytometry, scanning electron microscopy, and x-ray diffraction crystallography. Knowledge generated by this research has the potential to guide building maintenance practices to prevent the proliferation of Legionella.

提案编号：1706733PI名称：Marc A. Edwards饮用水中不断增长的公共卫生威胁（OPS），例如Legionella，呼吁与可持续性目标兼容的实用工程解决方案。利用广泛用于管道系统的铜（CU）管道的自然消毒特性是控制军团菌的一种有吸引力的方法。但是，CU在实践中并不始终有效，因为它有时会抑制，而其他时候刺激了军团菌的生长。 这项研究旨在通过基准，试点和现场规模研究来解决这一基本知识差距，以确定增强或抑制军团菌的CU驱动消毒的水化学物质。这项研究具有潜在的变革性，因为它将为解决关键公共卫生威胁的实用，经济和可持续性的方式提供信息，就像将数万亿美元投资用于升级过时的水基础设施一样。这项研究的总体假设是水化学直接和间接影响Cu毒性对cumionella菌的毒性。具体目标是：1）确定在与热水建筑水暖系统有关的条件下，在控制对军团菌的毒性中的CU形成，络合和降水的影响； 2）探索居住在管道系统中的微生物群落在介导Cu的毒性中的作用，例如，通过屏蔽或应对腐蚀反应（例如H2进化）释放的营养物质，并确认目标1在现实的目标试验性尺度管道条件下的现象； 3）进行现场调查，以验证现实世界中的水管系统中的物理化学，CU形成性/溶解度和微生物因子之间的关系，并照亮了Cu增强的驱动因素与CU抑制军团菌的生长。 这项研究将是对饮用水系统中Cu毒性的物种和溶解度依赖性性质的首次系统检查，尤其是在热水中与OP增殖最相关的热水中。特别有趣的是库（Cu1+）离子的发生和行为。 Cu1+可能对军团菌无毒，甚至可能通过催化较不贵金属水管材料的沉积腐蚀而间接刺激其生长，然后将H2提供H2用于自养氢氧化细菌。铜（Cu2+）离子具有很强的杀生物特性，但有效性可能受到温度，氧化还原，无机和有机配体，腐蚀控制剂和生物膜特性的限制。工具的创新组合将用于表征微生物群落组成，物理化学特性，CU形成和军团菌中的复杂相互依赖性，包括下一代DNA测序，绿色荧光蛋白修饰的菌株，流式细胞仪，扫描电子显微镜和X-RAY差异差异。这项研究产生的知识有可能指导建筑物维护实践，以防止军团菌的扩散。

项目成果

Shotgun Metagenomics Reveals Taxonomic and Functional Shifts in Hot Water Microbiome Due to Temperature Setting and Stagnation

• DOI: 10.3389/fmicb.2018.02695
• 发表时间: 2018-11-13
• 期刊: FRONTIERS IN MICROBIOLOGY
• 影响因子: 5.2
• 作者: Dai, Dongjuan;Rhoads, William J.;Pruden, Amy
• 通讯作者: Pruden, Amy

Natural Organic Matter, Orthophosphate, pH, and Growth Phase Can Limit Copper Antimicrobial Efficacy for Legionella in Drinking Water

• DOI: 10.1021/acs.est.0c06804
• 发表时间: 2021-01-11
• 期刊: ENVIRONMENTAL SCIENCE & TECHNOLOGY
• 影响因子: 11.4
• 作者: Song, Yang;Pruden, Amy;Rhoads, William J.
• 通讯作者: Rhoads, William J.

Replicable simulation of distal hot water premise plumbing using convectively-mixed pipe reactors

• DOI: 10.1371/journal.pone.0238385
• 发表时间: 2020-09
• 期刊: PLoS ONE
• 影响因子: 3.7
• 作者: M. S. Spencer;Abraham Cullom;W. Rhoads;A. Pruden;Marc A Edwards